Osteopontin (SPP1) antibody

Antigen: Osteopontin (SPP1)

Synonyms: OPN, BNSP, BSPI, ETA-1, MGC110940

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Dot Blot (Dot), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN148612

Additional Information

Alternative name: Osteopontin / SPP1

Immunogen: Peptide: CKSKKFRRPDIQYPD, also available as GTX29078.

Format: Serum

Isotype: IgG  (Matching secondary antibodies)

Description: Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors (ref 1). OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype (ref 1-4). Targeting of the gene encoding OPN, spp1 , has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions (ref 5, 6), loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth (ref 7-12). The fact that no other proteins seem to share a redundant activity with OPN under these conditions suggests that OPN has a unique functional role during tissue injury and stress. Interestingly, several members of the matrix metalloproteinase (MMP) family are also induced during inj

Specificity: This antibody recognises the full length osteopontin protein (which runs at 66 kD on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. This antibody recognises the 32 kD MMP-cleaved C-fragment, but not the 40 kD N-termin

Application Details

Application Notes: Western blot: at 1/1000 will strongly detect approximately 250 ng of OPN protein on a blot. Immunohistochemistry: use at 1/100-1/300. No pretreatment is required.Researchers should determine optimal titers for other applications.

Concentration: Batch Dependent

Purity: Whole antiserum

Buffer: serum with 0.02% sodium azide

Storage: Store vial at -20 C prior to opening. Aliquot contents and freeze at -20 C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for :

Research Area: Hormones, Enzymes, Metabolism, Extracellular Matrix

Restrictions: For Research Use only

Images

anti-Osteopontin (SPP1) antibody