RNF139 antibody (AA 651-663)

Catalog No. ABIN1492761

Product Details anti-RNF139 Antibody

Target: RNF139 (Ring Finger Protein 139 (RNF139))

Binding Specificity: AA 651-663

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This RNF139 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RNF139.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Rabbit (92%) Marmoset, Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HTGAAAEEFNDDT from the C-terminus of human RNF139 (NP_009149.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Rabbit (92%), Marmoset, Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:16000), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Experiments gave bands at approx. 150  kDa and 50  kDa in Human Skeletal Mucle lysate after 0.5 μg/mL antibody staining. This protein has a calculated MW of 76.0  kDa according to NP_009149.2.The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-1.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RNF139

Target: RNF139 (Ring Finger Protein 139 (RNF139))

Alternative Name: RNF139 / TRC8 (RNF139 Products)

Synonyms: HRCA1 antibody, RCA1 antibody, TRC8 antibody, 4930555P18Rik antibody, zgc:175173 antibody, rnf139 antibody, ring finger protein 139 antibody, ring finger protein 139 S homeolog antibody, RNF139 antibody, Rnf139 antibody, rnf139 antibody, rnf139.S antibody

Background: Name/Gene ID: RNF139

Synonyms: RNF139, HRCA1, RCA1, TRC8, Ring finger protein 139

Gene ID: 11236

NCBI Accession: NP_009149

UniProt: Q8WU17