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Product details for SOX4 (Human) antibody
SOX4 (Human) antibody
Overview
| Antigen: | SOX4 |
| Antibody Type: | Polyclonal |
| Application: | Immunocytochemistry (ICC), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunoprecipitation (IP), Western Blotting (WB) |
| Reactivity: | Human, Mouse (Murine), Rat |
| Host: | Rabbit |
| Quantity: | |
| Price: | 271,00 € (Plus shipping costs and VAT) |
| Order number: | ABIN149300 |
| Availability: | 7 to 10 days until shipment |
Additional Information: SOX4 (Human) antibody
| Antigen | SOX4 |
| Immunogen | Synthetic peptide: PTPGSTASTGGKADDPS conjugated to BSA, corresponding to amino acids 34-50 of Human SOX4. |
| Reactivity | Human, Mouse (Murine), Rat |
| Antibody Type | Polyclonal |
| Isotype | IgG »Matching secondary antibodies |
| Description | The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. |
| Host | Rabbit |
Application Details: SOX4 (Human) antibody
| Application | Immunocytochemistry (ICC), Immunohistochemistry ((Frozen Sections) (IHC) (fro)), Immunoprecipitation (IP), Western Blotting (WB) |
| Application Notes | ICC: Use at a dilution of 1/200-1/1000. IHC-Fr: Use at a dilution of 1/200-1/1000. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/200-1/1000. Predicted molecular weight: 47.3 kDa. Method 1 for immunostaining and immunoblotting (Western blot)1. Carefully resuspend antibody pellet to uniformity.2. Remove a fixed amount of suspension and dissolve 1/5 in PBS or TBS to yield a 100 ug/ml solution. Method 2 for immunoprecipitation, supershift, immunostaining and immunoblotting (Western blot)1. Pellet antibodies at 10,000-15,000 x g for 10 minutes at 2 to 8°,C using a microcentrifuge.2. Carefully remove as much supernatant as possible. It is not necessary to remove all the ammonium sulfate solution, a small residual amount will not effect the antibody preparation. Dissolve the pellet (antibody) in small volume (50 uL) of PBS (or TBS) at final concentration of 1 mg/ml (50 ug/50 ul). Do not allow the pellet to dry out. This can cause loss of activity. Gently allow pellet to dissolve at least 1 hour before use. Do not vortex. Mix by finger-tapping or gentle stirring. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Concentration | 1 mg/ml |
| Purity | Immunogen affinity purified |
| Buffer | Preservative: NoneConstituents: 50% Ammonium sulphate, PBS |
| Restrictions | For research use only |
External Information: SOX4 (Human) antibody
| Google Scholar | Find more references for antigen SOX4 on Google Scholar™ |
| EMBL Harvester | Search human, mouse or rat genome for antigen SOX4 (Bioinformatic Harvester (EMBL)) |
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