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Histone Deacetylase 2 (HDAC2) (AA 471-488) antibody

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Synonyms HD2, RPD3, YAF1, D10Wsu179e, Yy1bp, mRPD3, HISTONE DEACETYLASE, T1N24.9, T1N24_9, histone deacetylase 2
AA 471-488
(47), (45), (15), (15), (12), (8), (8), (5), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(220), (110), (93), (6), (6), (5), (3), (2), (2), (2)
(205), (17), (3)
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)
(187), (84), (71), (63), (44), (23), (22), (20), (19), (6), (3), (3), (2), (1)
Pubmed 11 references available
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Quantity 50 μL
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Immunogen Synthetic peptide: C-SGEKTDTKGTKSEQLSNP conjugated to KLH, corresponding to amino acids 471-488 of Human HDAC 2.
Isotype IgG
Specificity Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kD). The sequence recognized by the antibody is highly conserved in mouse and chicken.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics Cellular Localization: Nuclear
Purification IgG fraction. Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins..
Alternative Name HDAC2 (HDAC2 Antibody Abstract)
Background Reversible acetylation of highly conserved lysine residues in N-terminal tail domains of core histones plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone acetylation is a dynamic process determined by the net activities of histone acetyltransferases (HATs) and the competing enzymes histone deacetylases (HDACs) (Taunton et al.). Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations (Wolffe 1996 and Grunstein 1997).
Synonyms: 605164, YAF1, RPD3, Q92769, HDAC2, 3066
Research Area Cardiovascular, Hypertrophy, DNA/RNA, Transcription Factors, Wnt Signaling
Pathways Neurotrophin Signaling Pathway
Application Notes ChIP: Use 2ul for 10^6 cells. Dot: Use at an assay dependent dilution. IHC-P: Use at a dilution of 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. IP: Use at a dilution of 1/2500. WB: Use at a dilution of 1/2000-1/20000. Predicted molecular weight: 55.3 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Concentration 12.4 mg/ml
Buffer 0.01M PBS pH7.4, 15mM Na azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
Supplier Images
Western Blotting (WB) image for anti-Histone Deacetylase 2 (HDAC2) (AA 471-488) antibody (ABIN150633) anti-Histone Deacetylase 2 (HDAC2) (AA 471-488) antibody
Background publications Grozinger, Hassig, Schreiber: "Three proteins define a class of human histone deacetylases related to yeast Hda1p." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 9, pp. 4868-73, 1999 (PubMed).

Fischle, Emiliani, Hendzel et al.: "A new family of human histone deacetylases related to Saccharomyces cerevisiae HDA1p." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 11713-20, 1999 (PubMed).

Tong, Hassig, Schnitzler et al.: "Chromatin deacetylation by an ATP-dependent nucleosome remodelling complex." in: Nature, Vol. 395, Issue 6705, pp. 917-21, 1998 (PubMed).

Zhang, LeRoy, Seelig et al.: "The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities." in: Cell, Vol. 95, Issue 2, pp. 279-89, 1998 (PubMed).

Hassig, Tong, Fleischer et al.: "A role for histone deacetylase activity in HDAC1-mediated transcriptional repression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 7, pp. 3519-24, 1998 (PubMed).

Lin, Nagy, Inoue et al.: "Role of the histone deacetylase complex in acute promyelocytic leukaemia." in: Nature, Vol. 391, Issue 6669, pp. 811-4, 1998 (PubMed).

Grunstein: "Histone acetylation in chromatin structure and transcription." in: Nature, Vol. 389, Issue 6649, pp. 349-52, 1997 (PubMed).

Taunton, Hassig, Schreiber: "A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p." in: Science (New York, N.Y.), Vol. 272, Issue 5260, pp. 408-11, 1996 (PubMed).

Wolffe: "Histone deacetylase: a regulator of transcription." in: Science (New York, N.Y.), Vol. 272, Issue 5260, pp. 371-2, 1996 (PubMed).

Lee, Hayes, Pruss et al.: "A positive role for histone acetylation in transcription factor access to nucleosomal DNA." in: Cell, Vol. 72, Issue 1, pp. 73-84, 1993 (PubMed).

Bauer, Hayes, White et al.: "Nucleosome structural changes due to acetylation." in: Journal of molecular biology, Vol. 236, Issue 3, pp. 685-90, 1994 (PubMed).

Catalog No. ABIN150633
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