RING1 and YY1 Binding Protein (RYBP) antibody

Details for Product No. ABIN150791
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Antigen
Synonyms rybp, AAP1, DEDAF, YEAF1, 2410018J24Rik, aap1, dedaf, yeaf1, cb337, wu:fi34f03
Reactivity
Human, Mouse (Murine), Rat (Rattus)
(67), (31), (28), (12), (12), (11), (2), (2)
Host
Rabbit
(56), (12)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(57), (17), (10), (9), (9), (7), (6), (6), (1), (1)
Pubmed 2 references available
Quantity 50 µg
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Catalog No. ABIN150791
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Immunogen Available on request
Isotype IgG
Specificity This antibody detects ~32kDa DEDAF.
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification Immunogen affinity purified.
Alternative Name RYBP
Background Cell death signal are transduced by Death Domain (DD), Death Effector Domain (DED), and Caspase Recruitment Domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA binding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Synonyms: DEDAF, Q8N488, AAP1, YEAF1, RYBP, 607535, 23429
Application Notes WB: Use at a concentration of 1.0 ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: A549 or HepG2 cell lysate

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Lefkowitz: "Identification of adenylate cyclase-coupled beta-adrenergic receptors with radiolabeled beta-adrenergic antagonists." in: Biochemical pharmacology, Vol. 24, Issue 18, pp. 1651-8, 1976 (PubMed).

Kawai, Nomura, Hoshino et al.: "Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity." in: Oncogene, Vol. 18, Issue 23, pp. 3471-80, 1999 (PubMed).

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