RING1 and YY1 Binding Protein (RYBP) antibody

Antigen: RING1 and YY1 Binding Protein (RYBP)

Synonyms: AAP1, DEDAF, YEAF1, MGC62213, 2410018J24Rik, rybp

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN150791

Additional Information

Alternative name: RYBP

Immunogen: Available on request

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG

Description: Cell death signal are transduced by Death Domain (DD), Death Effector Domain (DED), and Caspase Recruitment Domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and augments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA binding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.

Specificity: This antibody detects ~32kDa DEDAF.

Application Details

Application Notes: WB: Use at a concentration of 1.0 ug/ml. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Concentration: 0.5 mg/ml

Purification: Immunogen affinity purified

Buffer: PBS pH 7.2 with 50% glycerol

Preservative: 1% BSA and 0.02% sodium azide

Storage: Store at -20 C. For long term storage, aliquot and refreeze at -70 C. Avoid repeated freeze/defrost cycles

Restrictions: For Research Use only

Publications

Lefkowitz: "Identification of adenylate cyclase-coupled beta-adrenergic receptors with radiolabeled beta-adrenergic antagonists." in: Biochemical pharmacology, Vol. 24, Issue 18, pp. 1651-8, 1976 (PubMed).

Kawai, Nomura, Hoshino et al.: "Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity." in: Oncogene, Vol. 18, Issue 23, pp. 3471-80, 1999 (PubMed).