Catenin beta-1 (CTNB1) (AA 768-781) antibody

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Antigen
Synonyms Bfc, Catnb, Mesc, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, CHBCAT
Epitope
AA 768-781
(2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(9), (3), (2)
Host
Rabbit
(9), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1)
Application
Dot Blot (DB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(12), (7), (6), (6), (3), (2), (2), (1), (1)
Pubmed 14 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN150872
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Immunogen Synthetic peptide: PGDSNQLAWFDTDL conjugated to KLH, corresponding to amino acids 768-781 of Human or mouse beta Catenin.
Isotype IgG
Specificity Reacts in dot blot with beta-catenin peptide 768-781 conjugated to BSA.In immunoblots, reacts with a 94kD protein in extracts of Madin-Darby Bovine Kidney (MDBK) cultured cells. Specific staining is inhibited following pre-incubation of the antiserum with the beta-catenin peptide.Shows no reactivity with BSA conjugated alpha catenin peptide (amino acids 890-901).
Cross-Reactivity Mouse (Murine), Fish, Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Brachydanio rerio)
Characteristics Cellular Localization: Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation).
Purification Whole antiserum. Delipidized antiserum..
Alternative Name Catenin beta-1
Background The distinct peripheral cytosolic proteins, alpha, beta and gamma-catenin (102, 94 and 86 kD) found in many tissues (1,2,3) bind to the conserved cytoplasmic tail domain of the cell-adhesion cadherins. Catenins link E-cadherin to other integral membrane or cytoplasmic proteins and are modulated by Wnt-1 proto-oncogene. (6,7) They are good candidates for mediating transduction of cell-cell contact positional signals to the cell interior. (4,5) Absence of alpha-catenin is found in certain tumor cell lines (8) and reduced levels in certain human carcinomas (9). Beta-catenin binds directly to the cytoplasmic tail of E-cadherin. It binds to the N-terminus of alpha-catenin and interacts with the protein product of the tumor suppressor gene APC. (10) This interaction involves a 15-aa repeat in the APC. (11) Beta-catenin cell levels seem to be controlled by APC. (12) The central core region of beta-catenin is involved in mediation of cadherin-catenin complex interaction with EGFR. (13)
Synonyms: CTNNB1, P35222, 114550, 1499, beta Catenin, CTNNB
Research Area Signaling
Application Notes Dot: Use at an assay dependant dilution. IHC-Fr: Use at a dilution of 1/2000 (bovine kidney frozen sections or cultured MDBK cells). WB: Use at a dilution of 1/4000 (MDBK cells extract). Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Comment

Positive Controls: MDBK cells

Restrictions For Research Use only
Format Liquid
Buffer Preservative: 15mM Sodium Azide, Constituents: Whole Serum
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C
Storage Comment Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below.
General Ozawa, Ringwald, Kemler: "Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 11, pp. 4246-50, 1990 (PubMed).

Nagafuchi, Takeichi: "Transmembrane control of cadherin-mediated cell adhesion: a 94 kDa protein functionally associated with a specific region of the cytoplasmic domain of E-cadherin." in: Cell regulation, Vol. 1, Issue 1, pp. 37-44, 1992 (PubMed).

Ozawa, Baribault, Kemler: "The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species." in: The EMBO journal, Vol. 8, Issue 6, pp. 1711-7, 1989 (PubMed).

Cowin, Kapprell, Franke et al.: "Plakoglobin: a protein common to different kinds of intercellular adhering junctions." in: Cell, Vol. 46, Issue 7, pp. 1063-73, 1986 (PubMed).

Reynolds, Daniel, McCrea et al.: "Identification of a new catenin: the tyrosine kinase substrate p120cas associates with E-cadherin complexes." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8333-42, 1994 (PubMed).

Munemitsu, Albert, Souza et al.: "Regulation of intracellular beta-catenin levels by the adenomatous polyposis coli (APC) tumor-suppressor protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 7, pp. 3046-50, 1995 (PubMed).

Hoschuetzky, Aberle, Kemler: "Beta-catenin mediates the interaction of the cadherin-catenin complex with epidermal growth factor receptor." in: The Journal of cell biology, Vol. 127, Issue 5, pp. 1375-80, 1994 (PubMed).

Cowin: "Unraveling the cytoplasmic interactions of the cadherin superfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 23, pp. 10759-61, 1994 (PubMed).

Hinck, Nelson, Papkoff: "Wnt-1 modulates cell-cell adhesion in mammalian cells by stabilizing beta-catenin binding to the cell adhesion protein cadherin." in: The Journal of cell biology, Vol. 124, Issue 5, pp. 729-41, 1994 (PubMed).

Schäfer, Koch, Franke: "Identification of the ubiquitous human desmoglein, Dsg2, and the expression catalogue of the desmoglein subfamily of desmosomal cadherins." in: Experimental cell research, Vol. 211, Issue 2, pp. 391-9, 1994 (PubMed).

Shiozaki, Iihara, Oka et al.: "Immunohistochemical detection of alpha-catenin expression in human cancers." in: The American journal of pathology, Vol. 144, Issue 4, pp. 667-74, 1994 (PubMed).

Tsukita, Itoh, Nagafuchi et al.: "Submembranous junctional plaque proteins include potential tumor suppressor molecules." in: The Journal of cell biology, Vol. 123, Issue 5, pp. 1049-53, 1994 (PubMed).

Su, Vogelstein, Kinzler: "Association of the APC tumor suppressor protein with catenins." in: Science (New York, N.Y.), Vol. 262, Issue 5140, pp. 1734-7, 1994 (PubMed).

Bradley, Cowin, Brown: "Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion." in: The Journal of cell biology, Vol. 123, Issue 6 Pt 2, pp. 1857-65, 1994 (PubMed).

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