You are viewing an incomplete version of our website. Please click to reload the website as full version.

ATM antibody (Ataxia Telangiectasia Mutated) (C-Term)

Details for Product anti-ATM Antibody No. ABIN151030, Supplier: Log in to see
Antigen
  • AI256621
  • at1
  • AT1
  • ATA
  • ATC
  • ATD
  • ATDC
  • atdc
  • ATE
  • Atm
  • ATM
  • atm
  • atm/tefu
  • C030026E19Rik
  • CG6535
  • dATM
  • Dmel\\CG6535
  • tef
  • Tefu
  • tel1
  • TEL1
  • TELO1
  • telo1
  • Xatm
Alternatives
anti-Human ATM antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
83
40
17
16
14
14
13
8
7
7
7
6
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
340
127
65
14
7
4
1
Host
Rabbit
222
92
17
8
3
Clonality
Polyclonal
Conjugate
This ATM antibody is un-conjugated
11
11
10
9
6
6
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
207
121
101
97
83
75
59
25
22
15
12
4
4
3
2
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Immunogen A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence). [UniProt# Q13315]
Purification Immunogen affinity purified
Alternative Name ATM (ATM Antibody Abstract)
Background Gene Symbol: ATM
Molecular Weight Theoretical MW: 350 kDa
Gene ID 472
UniProt Q13315
Research Area Cancer, DNA/RNA
Pathways p53 Signaling, Apoptosis, DNA Damage Repair
Application Notes Western Blot 1:500-1:1000, Flow Cytometry, ELISA, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:100
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization
1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase
. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.
. Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes
. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/mL
Buffer PBS, 50 % glycerol
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in HeLa whole cell lysate ...
Immunofluorescence (IF) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Immunohistochemical staining using ABIN151030.
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 second exposure)
Western Blotting (WB) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in Raji whole cell lysate.
Flow Cytometry (FACS) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Flow Cytometry: ATM Antibody [ABIN151030] - Analysis of PE conjugate of ABIN151030. A...
Immunohistochemistry (IHC) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Immunohistochemistry: ATM Antibody [ABIN151030] - Staining of Testis, Seminiferous Tu...
Immunofluorescence (IF) image for anti-ATM antibody (Ataxia Telangiectasia Mutated) (C-Term) (ABIN151030) Immunocytochemistry/Immunofluorescence: ATM Antibody [ABIN151030] - HeLa cells were f...
Product cited in: Qi, Qiu, Gu, Tian, Zhang: "ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts." in: Scientific reports, Vol. 6, pp. 24700, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Nagy, Kalousi, Furst, Koch, Fischer, Soutoglou: "Tankyrases Promote Homologous Recombination and Check Point Activation in Response to DSBs." in: PLoS genetics, Vol. 12, Issue 2, pp. e1005791, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Parplys, Zhao, Sharma, Groesser, Liang, Maranon, Leung, Grundt, Dray, Idate, Østvold, Schild, Sung, Wiese: "NUCKS1 is a novel RAD51AP1 paralog important for homologous recombination and genome stability." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9817-34, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Murata, Uehara, Hosoi: "Activation of mTORC1 under nutrient starvation conditions increases cellular radiosensitivity in human liver cancer cell lines, HepG2 and HuH6." in: Biochemical and biophysical research communications, Vol. 468, Issue 4, pp. 684-90, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Yoon, Ishdorj, Graham, Johnston, Gibson: "Valproic acid enhances fludarabine-induced apoptosis mediated by ROS and involving decreased AKT and ATM activation in B-cell-lymphoid neoplastic cells." in: Apoptosis : an international journal on programmed cell death, Vol. 19, Issue 1, pp. 191-200, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Morita, Tanimoto, Murakami, Morinaga, Hosoi: "Mitochondria are required for ATM activation by extranuclear oxidative stress in cultured human hepatoblastoma cell line Hep G2 cells." in: Biochemical and biophysical research communications, Vol. 443, Issue 4, pp. 1286-90, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Silva, Stambaugh, Yokomori, Shah, Berns: "DNA damage to a single chromosome end delays anaphase onset." in: The Journal of biological chemistry, Vol. 289, Issue 33, pp. 22771-84, 2014 (PubMed).

Brinley, Theriot, Nelman-Gonzalez, Crucian, Stowe, Barrett, Pierson: "Characterization of Epstein-Barr virus reactivation in a modeled spaceflight system." in: Journal of cellular biochemistry, Vol. 114, Issue 3, pp. 616-24, 2013 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Gautam, Bridge: "The kinase activity of ataxia-telangiectasia mutated interferes with adenovirus E4 mutant DNA replication." in: Journal of virology, Vol. 87, Issue 15, pp. 8687-96, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Rankin, Sarkar, Faller: "Mechanism of T-oligo-induced cell cycle arrest in Mia-PaCa pancreatic cancer cells." in: Journal of cellular physiology, Vol. 227, Issue 6, pp. 2586-94, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Jacquemin, Rieunier, Jacob, Bellanger, dEnghien, Laugé, Stoppa-Lyonnet, Stern: "Underexpression and abnormal localization of ATM products in ataxia telangiectasia patients bearing ATM missense mutations." in: European journal of human genetics : EJHG, Vol. 20, Issue 3, pp. 305-12, 2012 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Nguyen, Possemato, Bauerlein, Xie, Scully, Hahn: "Nek4 regulates entry into replicative senescence and the response to DNA damage in human fibroblasts." in: Molecular and cellular biology, Vol. 32, Issue 19, pp. 3963-77, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Xie, Li, Xing, Tian, Yin, He, Zhang: "ATM-mediated NuSAP phosphorylation induces mitotic arrest." in: Biochemical and biophysical research communications, Vol. 404, Issue 1, pp. 413-8, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Wu, Wei, Hong, Chiang: "Inactivation of ataxia telangiectasia mutated gene can increase intracellular reactive oxygen species levels and alter radiation-induced cell death pathways in human glioma cells." in: International journal of radiation biology, Vol. 87, Issue 4, pp. 432-42, 2011 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Dixon, Henry, Siroky, Chu, Groen, Bissler: "Cell cycle control and DNA damage response of conditionally immortalized urothelial cells." in: PLoS ONE, Vol. 6, Issue 1, pp. e16595, 2011 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Du, Kayali, Bertoni, Fike, Hu, Iversen, Gatti: "Arginine-rich cell-penetrating peptide dramatically enhances AMO-mediated ATM aberrant splicing correction and enables delivery to brain and cerebellum." in: Human molecular genetics, Vol. 20, Issue 16, pp. 3151-60, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Yang, Chou, Pan, Chen, Tsou, Yeh, Cheung, Chen, Chang: "Enhancement of non-homologous end joining DNA repair capacity confers cancer cells resistance to the novel selenophene compound, D-501036." in: Cancer letters, Vol. 309, Issue 1, pp. 110-8, 2011 (PubMed). (Sample species: Human). Further details: Western Blotting

Hu, Du, Nagabayashi, Seeger, Gatti: "ATM is down-regulated by N-Myc-regulated microRNA-421." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 4, pp. 1506-11, 2010 (PubMed). (Sample species: Human). Further details: Western Blotting,ELISA

Macůrek, Lindqvist, Voets, Kool, Vos, Medema: "Wip1 phosphatase is associated with chromatin and dephosphorylates gammaH2AX to promote checkpoint inhibition." in: Oncogene, Vol. 29, Issue 15, pp. 2281-91, 2010 (PubMed). (Sample species: Human).

Karimi-Busheri, Rasouli-Nia, Mackey, Weinfeld: "Senescence evasion by MCF-7 human breast tumor-initiating cells." in: Breast cancer research : BCR, Vol. 12, Issue 3, pp. R31, 2010 (PubMed). (Sample species: Human). Further details: Immunohistochemistry