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Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody

Details for Product No. ABIN151030
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Antigen
Synonyms AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1, AI256621, C030026E19Rik, ATM, Atm, CG6535, Dmel\\CG6535, Tefu, atm, atm/tefu, dATM, tef, Xatm, at1, atdc, tel1, telo1
Epitope
C-Term
(55), (19), (12), (12), (8), (8), (5), (5), (4), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(226), (67), (39), (4), (4), (1), (1)
Host
Rabbit
(164), (44), (16), (6), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(7), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(128), (61), (50), (46), (45), (40), (33), (13), (8), (5), (2), (2)
Pubmed 15 references available
Quantity 0.05 mL
Shipping to United States (Change)
Catalog No. ABIN151030
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Immunogen A fragment of the human ATM protein corresponding to the C-terminus (within the last third of the protein sequence).
Purification affinity purified
Alternative Name ATM
Background Ataxia-telangiectasia (A-T) is a recessive childhood disease caused by mutations in theATM (AT-mutated) gene. Symptoms include neurological abnormalities that causeunsteady posture, dilated blood vessels, infertility, radiation sensitivity, immunedeficiencies and lymphoid malignancies. It appears that the diverse defects seen inATM null mammals are manifestations of disparate signal transduction defects. The ATM protein is related to a family of proteins through a C-terminalphoshatidylinositol 3-kinase (PI3-kinase) domain. Members of the PI3-kinase family ofproteins are involved in cell cycle control, DNA replication, recombination and repair.ATM also shares sequence homology with portions of the yeast RAD3 gene. The mainrole of ATM appears to be induction of a DNA-damage control pathway in response togenotoxic insults, such as ionizing radiation or anti-tumor medications and theprogrammed DNA breaks of meiosis. ATM has also been linked to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.(Rogoff et. al.). Alternate Names: anti-AT complementation group A antibody, anti-AT complementation group C antibody,anti-AT complementation group D antibody, anti-AT complementation group E antibody,anti-AT mutated protein antibody, anti-AT1 antibody, anti-ATA antibody, anti-Ataxiatelangiectasia gene mutated antibody, anti-AT protein antibody, anti-ATC antibody,anti-ATDC antibody, anti-ATE antibody, anti-MGC74674 antibody, anti-Serine proteinkinase ATM antibody, anti-T cell prolymphocytic leukemia antibody, anti-TEL1 antibody,anti-TPLL antibody, anti-TELO1 antibody.
Gene Symbol: ATM
Gene ID 472
UniProt Q13315
Research Area Cancer, DNA/RNA
Application Notes This ATM antibody can be used for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western Blot. In WB, it detects a band at ~350 kDa, representing ATM.
Recommended dilutions: ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:100, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation 1:10-1:500, Western Blot 1:500-1:1000
Protocol Immunohistochemistry-FFPE sections protocol specific for ATM Antibody Immunohistochemistry- FFPE sectionsDeparaffinization
1. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.
. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.Quench Endogenous Peroxidase
. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.
. Place slides in distilled water: 2 changes for 2 minutes each.Retrieve Epitopes
. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 2.5 mg/mL
Buffer PBS, 30 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody Western Blot: ATM Antibody [ABIN151030] - Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 sec. exposure)
anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (2) Immunohistochemical staining using ABIN151030.
anti-Ataxia Telangiectasia Mutated (ATM) (C-Term) antibody (3) Detection of ATM in HeLa whole cell lysate using ABIN151030. (30 second exposure)
General Vemuri, Schiller, Naegele: "Elevated DNA double strand breaks and apoptosis in the CNS of scid mutant mice." in: Cell death and differentiation, Vol. 8, Issue 3, pp. 245-55, 2005 (PubMed).

Oakley, Loberg, Yao et al.: "UV-induced hyperphosphorylation of replication protein a depends on DNA replication and expression of ATM protein." in: Molecular biology of the cell, Vol. 12, Issue 5, pp. 1199-213, 2001 (PubMed).

Stracker, Carson, Weitzman: "Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex." in: Nature, Vol. 418, Issue 6895, pp. 348-52, 2002 (PubMed).

Suorensen, Syljuuasen, Falck et al.: "Chk1 regulates the S phase checkpoint by coupling the physiological turnover and ionizing radiation-induced accelerated proteolysis of Cdc25A." in: Cancer cell, Vol. 3, Issue 3, pp. 247-58, 2003 (PubMed).

Cortez: "Caffeine inhibits checkpoint responses without inhibiting the ataxia-telangiectasia-mutated (ATM) and ATM- and Rad3-related (ATR) protein kinases." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37139-45, 2003 (PubMed).

Cerosaletti, Concannon: "Independent roles for nibrin and Mre11-Rad50 in the activation and function of Atm." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38813-9, 2004 (PubMed).

Butch, Chun, Nahas et al.: "Immunoassay to measure ataxia-telangiectasia mutated protein in cellular lysates." in: Clinical chemistry, Vol. 50, Issue 12, pp. 2302-8, 2004 (PubMed).

Out, Hoekstra, de Jager et al.: "Adenovirus-mediated hepatic overexpression of scavenger receptor class B type I accelerates chylomicron metabolism in C57BL/6J mice." in: Journal of lipid research, Vol. 46, Issue 6, pp. 1172-81, 2005 (PubMed).

Hovest, Brüggenolte, Hosseini et al.: "Senescence of human fibroblasts after psoralen photoactivation is mediated by ATR kinase and persistent DNA damage foci at telomeres." in: Molecular biology of the cell, Vol. 17, Issue 4, pp. 1758-67, 2006 (PubMed).

Hiyama, Katsura, Yoshihara et al.: "Haploinsufficiency of the Mus81-Eme1 endonuclease activates the intra-S-phase and G2/M checkpoints and promotes rereplication in human cells." in: Nucleic acids research, Vol. 34, Issue 3, pp. 880-92, 2006 (PubMed).

Cuadrado, Martinez-Pastor, Murga et al.: "ATM regulates ATR chromatin loading in response to DNA double-strand breaks." in: The Journal of experimental medicine, Vol. 203, Issue 2, pp. 297-303, 2006 (PubMed).

Gaspar, Shenk: "Human cytomegalovirus inhibits a DNA damage response by mislocalizing checkpoint proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 8, pp. 2821-6, 2006 (PubMed).

Yang, Jeong, Brown et al.: "Promyelocytic leukemia activates Chk2 by mediating Chk2 autophosphorylation." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26645-54, 2006 (PubMed).

Lovejoy, Lock, Yenamandra et al.: "DDB1 maintains genome integrity through regulation of Cdt1." in: Molecular and cellular biology, Vol. 26, Issue 21, pp. 7977-90, 2006 (PubMed).

Ho, Margossian, Taniguchi et al.: "Phosphorylation of FANCD2 on two novel sites is required for mitomycin C resistance." in: Molecular and cellular biology, Vol. 26, Issue 18, pp. 7005-15, 2006 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN151030
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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