Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Monoclonal (H1alpha67)

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN151033

Additional Information

Alternative name: HIF1A / HIF1 alpha

Gene ID: 3091

Immunogen: A fusion protein containing residues 432-528 of human HIF-1 alpha.

Cross-Reactivity: Cow (Bovine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)

Isotype: IgG2b

Clone: H1alpha67

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs asa post-translational modification by prolyl hydroxylation. HIF-1 is a heterodimercomposed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantlytranslated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated atPro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventuallydegraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation preventsHIF degradation and increases transcriptional activity. Therefore, the concentration ofHIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under eithercondition. HIF hydroxylases provide insight into hypoxic cell responses, which may beused to help isolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting protein antibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.

Specificity: Species Reactivity: Human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret HIF-1alpha. Otherspecies have not been tested. Localization: Nuclear.

Application Details

Application Notes: Immunohistochemistry 1:25, Western Blot 1:500, Immunohistochemistry-Frozen 1:100. Positive Controls: Nuclear extracts are recommended. COS-7 treated and untreated nuclear extracts for

Concentration: 4.0 mg/ml

Purification: Purified

Buffer: PBS. Preservative: 0.05%sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Western analysis of COS-7 nuclear extracts (50 ug) using ABIN151032.

Publications

Gothiue, Richard, Berra et al.: "Identification of alternative spliced variants of human hypoxia-inducible factor-1alpha." in: The Journal of biological chemistry, Vol. 275, Issue 10, pp. 6922-7, 2000 (PubMed).

Birner, Schindl, Obermair et al.: "Overexpression of hypoxia-inducible factor 1alpha is a marker for an unfavorable prognosis in early-stage invasive cervical cancer." in: Cancer research, Vol. 60, Issue 17, pp. 4693-6, 2000 (PubMed).

Birner, Schindl, Obermair et al.: "Expression of hypoxia-inducible factor 1alpha in epithelial ovarian tumors: its impact on prognosis and on response to chemotherapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 6, pp. 1661-8, 2001 (PubMed).

Birner, Gatterbauer, Oberhuber et al.: "Expression of hypoxia-inducible factor-1 alpha in oligodendrogliomas: its impact on prognosis and on neoangiogenesis." in: Cancer, Vol. 92, Issue 1, pp. 165-71, 2001 (PubMed).

Duyndam, Hulscher, Fontijn et al.: "Induction of vascular endothelial growth factor expression and hypoxia-inducible factor 1alpha protein by the oxidative stressor arsenite." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48066-76, 2001 (PubMed).

Aminova, Chavez, Lee et al.: "Prosurvival and prodeath effects of hypoxia-inducible factor-1alpha stabilization in a murine hippocampal cell line." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3996-4003, 2005 (PubMed).

Alberghini, Recalcati, Tacchini et al.: "Loss of the von Hippel Lindau tumor suppressor disrupts iron homeostasis in renal carcinoma cells." in: The Journal of biological chemistry, Vol. 280, Issue 34, pp. 30120-8, 2005 (PubMed).

Higami, Barger, Page et al.: "Energy restriction lowers the expression of genes linked to inflammation, the cytoskeleton, the extracellular matrix, and angiogenesis in mouse adipose tissue." in: The Journal of nutrition, Vol. 136, Issue 2, pp. 343-52, 2006 (PubMed).

Galabova-Kovacs, Matzen, Piazzolla et al.: "Essential role of B-Raf in ERK activation during extraembryonic development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1325-30, 2006 (PubMed).

Hsu, Adereth, Kose et al.: "Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility." in: The Journal of biological chemistry, Vol. 281, Issue 17, pp. 12069-80, 2006 (PubMed).

Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).

Fath, Kong, Liang et al.: "Histone deacetylase inhibitors repress the transactivation potential of hypoxia-inducible factors independently of direct acetylation of HIF-alpha." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13612-9, 2006 (PubMed).

Franco, Man, Chen et al.: "Targeted anti-vascular endothelial growth factor receptor-2 therapy leads to short-term and long-term impairment of vascular function and increase in tumor hypoxia." in: Cancer research, Vol. 66, Issue 7, pp. 3639-48, 2006 (PubMed).

Hu, Iyer, Sataur et al.: "Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1alpha) and HIF-2alpha in stem cells." in: Molecular and cellular biology, Vol. 26, Issue 9, pp. 3514-26, 2006 (PubMed).

Bracken, Fedele, Linke et al.: "Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environment." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 22575-85, 2006 (PubMed).

Bernhardt, Cuampean, Kany et al.: "Preconditional activation of hypoxia-inducible factors ameliorates ischemic acute renal failure." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 1970-8, 2006 (PubMed).

Chade, Zhu, Mushin et al.: "Simvastatin promotes angiogenesis and prevents microvascular remodeling in chronic renal ischemia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1706-8, 2006 (PubMed).

Asikainen, Chang, Coalson et al.: "Improved lung growth and function through hypoxia-inducible factor in primate chronic lung disease of prematurity." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1698-700, 2006 (PubMed).

Grimmer, Balbus, Lang et al.: "Regulation of type II collagen synthesis during osteoarthritis by prolyl-4-hydroxylases: possible influence of low oxygen levels." in: The American journal of pathology, Vol. 169, Issue 2, pp. 491-502, 2006 (PubMed).

Brooks, Wang, Yang et al.: "Characterization of cell clones isolated from hypoxia-selected renal proximal tubular cells." in: American journal of physiology. Renal physiology, Vol. 292, Issue 1, pp. F243-52, 2007 (PubMed).

Carroll, Kenerson, Yeung et al.: "Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors." in: Journal of virology, Vol. 80, Issue 21, pp. 10802-12, 2006 (PubMed).

Chavez, Baranova, Lin et al.: "The transcriptional activator hypoxia inducible factor 2 (HIF-2/EPAS-1) regulates the oxygen-dependent expression of erythropoietin in cortical astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 37, pp. 9471-81, 2006 (PubMed).

Chen, Yan, Davidson et al.: "Hypoxic stress induces dimethylated histone H3 lysine 9 through histone methyltransferase G9a in mammalian cells." in: Cancer research, Vol. 66, Issue 18, pp. 9009-16, 2006 (PubMed).

Groenman, Rutter, Caniggia et al.: "Hypoxia-inducible factors in the first trimester human lung." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 55, Issue 4, pp. 355-63, 2007 (PubMed).

Creighton-Gutteridge, Cardellina, Stephen et al.: "Cell type-specific, topoisomerase II-dependent inhibition of hypoxia-inducible factor-1alpha protein accumulation by NSC 644221." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 3, pp. 1010-8, 2007 (PubMed).

Bernhardt, Wiesener, Weidemann et al.: "Involvement of hypoxia-inducible transcription factors in polycystic kidney disease." in: The American journal of pathology, Vol. 170, Issue 3, pp. 830-42, 2007 (PubMed).

Chin, Jiang, Wegiel et al.: "Hypoxia-inducible factor 1alpha stabilization by carbon monoxide results in cytoprotective preconditioning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 12, pp. 5109-14, 2007 (PubMed).

Bateman, Tokunaga, Kareco et al.: "Myocardial hypoxia-inducible HIF-1alpha, VEGF, and GLUT1 gene expression is associated with microvascular and ICAM-1 heterogeneity during endotoxemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 1, pp. H448-56, 2007 (PubMed).