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Details for Product No. ABIN151033

Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

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Antigen
Synonyms HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hif1a, HIF1-alpha, HIF-1a, HIF1a, hif-1a
Reactivity
»Alternatives Cow (Bovine), Ferret, Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 28 references available
Catalog no. ABIN151033
Quantity 25 µL
Price
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Immunogen A fusion protein containing residues 432-528 of human HIF-1 alpha.
Clone H1alpha67
Isotype IgG2b
Specificity Species Reactivity: Human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret HIF-1alpha. Otherspecies have not been tested. Localization: Nuclear.
Cross-Reactivity Cow (Bovine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)
Purification Purified
Alternative Name HIF1A / HIF1 alpha
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs asa post-translational modification by prolyl hydroxylation. HIF-1 is a heterodimercomposed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantlytranslated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated atPro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventuallydegraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation preventsHIF degradation and increases transcriptional activity. Therefore, the concentration ofHIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under eithercondition. HIF hydroxylases provide insight into hypoxic cell responses, which may beused to help isolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting protein antibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.
Gene ID 3091
Application Notes Immunohistochemistry 1:25, Western Blot 1:500, Immunohistochemistry-Frozen 1:100. Positive Controls: Nuclear extracts are recommended. COS-7 treated and untreated nuclear extracts for
Restrictions For Research Use only
Format Liquid
Concentration 4.0 mg/ml
Buffer PBS. Preservative: 0.05%sodium azide.
Preservative Sodium azide
Storage 4 °C
Gothiue, Richard, Berra et al.: "Identification of alternative spliced variants of human hypoxia-inducible factor-1alpha." in: The Journal of biological chemistry, Vol. 275, Issue 10, pp. 6922-7, 2000 (PubMed).

Birner, Schindl, Obermair et al.: "Overexpression of hypoxia-inducible factor 1alpha is a marker for an unfavorable prognosis in early-stage invasive cervical cancer." in: Cancer research, Vol. 60, Issue 17, pp. 4693-6, 2000 (PubMed).

Birner, Schindl, Obermair et al.: "Expression of hypoxia-inducible factor 1alpha in epithelial ovarian tumors: its impact on prognosis and on response to chemotherapy." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 7, Issue 6, pp. 1661-8, 2001 (PubMed).

Birner, Gatterbauer, Oberhuber et al.: "Expression of hypoxia-inducible factor-1 alpha in oligodendrogliomas: its impact on prognosis and on neoangiogenesis." in: Cancer, Vol. 92, Issue 1, pp. 165-71, 2001 (PubMed).

Duyndam, Hulscher, Fontijn et al.: "Induction of vascular endothelial growth factor expression and hypoxia-inducible factor 1alpha protein by the oxidative stressor arsenite." in: The Journal of biological chemistry, Vol. 276, Issue 51, pp. 48066-76, 2001 (PubMed).

Aminova, Chavez, Lee et al.: "Prosurvival and prodeath effects of hypoxia-inducible factor-1alpha stabilization in a murine hippocampal cell line." in: The Journal of biological chemistry, Vol. 280, Issue 5, pp. 3996-4003, 2005 (PubMed).

Alberghini, Recalcati, Tacchini et al.: "Loss of the von Hippel Lindau tumor suppressor disrupts iron homeostasis in renal carcinoma cells." in: The Journal of biological chemistry, Vol. 280, Issue 34, pp. 30120-8, 2005 (PubMed).

Higami, Barger, Page et al.: "Energy restriction lowers the expression of genes linked to inflammation, the cytoskeleton, the extracellular matrix, and angiogenesis in mouse adipose tissue." in: The Journal of nutrition, Vol. 136, Issue 2, pp. 343-52, 2006 (PubMed).

Galabova-Kovacs, Matzen, Piazzolla et al.: "Essential role of B-Raf in ERK activation during extraembryonic development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 5, pp. 1325-30, 2006 (PubMed).

Hsu, Adereth, Kose et al.: "Endocytic function of von Hippel-Lindau tumor suppressor protein regulates surface localization of fibroblast growth factor receptor 1 and cell motility." in: The Journal of biological chemistry, Vol. 281, Issue 17, pp. 12069-80, 2006 (PubMed).

Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).

Fath, Kong, Liang et al.: "Histone deacetylase inhibitors repress the transactivation potential of hypoxia-inducible factors independently of direct acetylation of HIF-alpha." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13612-9, 2006 (PubMed).

Franco, Man, Chen et al.: "Targeted anti-vascular endothelial growth factor receptor-2 therapy leads to short-term and long-term impairment of vascular function and increase in tumor hypoxia." in: Cancer research, Vol. 66, Issue 7, pp. 3639-48, 2006 (PubMed).

Hu, Iyer, Sataur et al.: "Differential regulation of the transcriptional activities of hypoxia-inducible factor 1 alpha (HIF-1alpha) and HIF-2alpha in stem cells." in: Molecular and cellular biology, Vol. 26, Issue 9, pp. 3514-26, 2006 (PubMed).

Bracken, Fedele, Linke et al.: "Cell-specific regulation of hypoxia-inducible factor (HIF)-1alpha and HIF-2alpha stabilization and transactivation in a graded oxygen environment." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 22575-85, 2006 (PubMed).

Bernhardt, Cuampean, Kany et al.: "Preconditional activation of hypoxia-inducible factors ameliorates ischemic acute renal failure." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 1970-8, 2006 (PubMed).

Chade, Zhu, Mushin et al.: "Simvastatin promotes angiogenesis and prevents microvascular remodeling in chronic renal ischemia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1706-8, 2006 (PubMed).

Asikainen, Chang, Coalson et al.: "Improved lung growth and function through hypoxia-inducible factor in primate chronic lung disease of prematurity." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1698-700, 2006 (PubMed).

Grimmer, Balbus, Lang et al.: "Regulation of type II collagen synthesis during osteoarthritis by prolyl-4-hydroxylases: possible influence of low oxygen levels." in: The American journal of pathology, Vol. 169, Issue 2, pp. 491-502, 2006 (PubMed).

Brooks, Wang, Yang et al.: "Characterization of cell clones isolated from hypoxia-selected renal proximal tubular cells." in: American journal of physiology. Renal physiology, Vol. 292, Issue 1, pp. F243-52, 2007 (PubMed).

Carroll, Kenerson, Yeung et al.: "Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors." in: Journal of virology, Vol. 80, Issue 21, pp. 10802-12, 2006 (PubMed).

Chavez, Baranova, Lin et al.: "The transcriptional activator hypoxia inducible factor 2 (HIF-2/EPAS-1) regulates the oxygen-dependent expression of erythropoietin in cortical astrocytes." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 37, pp. 9471-81, 2006 (PubMed).

Chen, Yan, Davidson et al.: "Hypoxic stress induces dimethylated histone H3 lysine 9 through histone methyltransferase G9a in mammalian cells." in: Cancer research, Vol. 66, Issue 18, pp. 9009-16, 2006 (PubMed).

Groenman, Rutter, Caniggia et al.: "Hypoxia-inducible factors in the first trimester human lung." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 55, Issue 4, pp. 355-63, 2007 (PubMed).

Creighton-Gutteridge, Cardellina, Stephen et al.: "Cell type-specific, topoisomerase II-dependent inhibition of hypoxia-inducible factor-1alpha protein accumulation by NSC 644221." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 13, Issue 3, pp. 1010-8, 2007 (PubMed).

Bernhardt, Wiesener, Weidemann et al.: "Involvement of hypoxia-inducible transcription factors in polycystic kidney disease." in: The American journal of pathology, Vol. 170, Issue 3, pp. 830-42, 2007 (PubMed).

Chin, Jiang, Wegiel et al.: "Hypoxia-inducible factor 1alpha stabilization by carbon monoxide results in cytoprotective preconditioning." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 12, pp. 5109-14, 2007 (PubMed).

Bateman, Tokunaga, Kareco et al.: "Myocardial hypoxia-inducible HIF-1alpha, VEGF, and GLUT1 gene expression is associated with microvascular and ICAM-1 heterogeneity during endotoxemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 293, Issue 1, pp. H448-56, 2007 (PubMed).

Alternatives for antigen "Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)", type "Antibodies"
Hosts (180), (135), (2)
Reactivities (277), (120), (98), (77), (52), (49), (30), (27), (20), (19), (16), (4), (2), (2), (1), (1), (1), (1), (1)
Applications (258), (95), (76), (71), (57), (55), (51), (30), (19), (13), (11), (10), (9), (6), (2), (2), (1), (1)
Conjugates (33), (20), (19), (12), (12), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (36), (24), (16), (11), (8), (8), (7), (6), (6), (6), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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