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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody
|Synonyms||HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a|
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|28 references available|
|Quantity||25 µl (4.0 mg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||HIF1A / HIF1 alpha|
|Immunogen||A fusion protein containing residues 432-528 of human HIF-1 alpha.|
|Cross-Reactivity||Cow (Bovine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Sheep (Ovine)|
|Description||Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs asa post-translational modification by prolyl hydroxylation. HIF-1 is a heterodimercomposed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantlytranslated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated atPro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventuallydegraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation preventsHIF degradation and increases transcriptional activity. Therefore, the concentration ofHIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under eithercondition. HIF hydroxylases provide insight into hypoxic cell responses, which may beused to help isolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting protein antibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.|
|Specificity||Species Reactivity: Human, monkey, sheep, mouse, rat, rabbit, pig, bovine and ferret HIF-1alpha. Otherspecies have not been tested. Localization: Nuclear.|
|Application Notes||Immunohistochemistry 1:25, Western Blot 1:500, Immunohistochemistry-Frozen 1:100. Positive Controls: Nuclear extracts are recommended. COS-7 treated and untreated nuclear extracts for|
|Buffer||PBS. Preservative: 0.05%sodium azide.|
|Storage||Store at 4C. Do not freeze.|
|Restrictions||For Research Use only|
|Western analysis of COS-7 nuclear extracts (50 ug) using ABIN151032.|
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