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OGG1 antibody (8-Oxoguanine DNA Glycosylase) (AA 1-100)

Details for Product anti-OGG1 Antibody No. ABIN151034, Supplier: Login to see New
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AA 1-100
(14), (11), (9), (7), (6), (4), (3), (3), (1), (1), (1), (1), (1)
Human, Monkey, Mouse (Murine), Rat (Rattus)
(75), (47), (45), (5), (2), (1), (1), (1)
(72), (20), (5)
This OGG1 antibody is un-conjugated
(5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(80), (25), (25), (15), (11), (6), (3), (2), (2)
Pubmed 15 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen A peptide derived from human Ogg1 (within amino acids 1-100).
Purification affinity purified
Alternative Name OGG1 (OGG1 Antibody Abstract)
Background 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causesG:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent themutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found tohave a functional yeast (yOgg1) and human (hOgg1) homologue.hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine fromDNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in thesusceptibility to cancer development. The hOgg1 gene has been mapped to region3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. Inparticular, 3p25-p26 is a common LOH region in lung cancer.Recent work has demonstrated that Ogg plays an important role in CAG expansion, acharacteristic of several neurodegenerative diseases. Ogg appears to be responsiblefor progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Oggprovides a direct link between DNA damage and toxicity in neurons. Alternate Names: anti-8-Oxoguanine DNA-Glycosylase antibody.
Gene Symbol: OGG1
Gene ID 4968, 81528
UniProt O15527
Pathways DNA Damage Repair
Application Notes This Ogg1 antibody is useful for Immunohistochemistry on frozen and paraffin-embedded sections, Immunoprecipitation Co-IP (PMID: 20956573), ELISA (PMID: 19506022) and Western Blot. In WB, it recognizes a band at ~39 kDa, representing Ogg1. In ICC/IF nuclear staining was observed in Hek293 cells.
Recommended dilutions: ELISA, Immunocytochemistry/Immunofluorescence 1:100 - 1:200, Immunohistochemistry, Immunohistochemistry-Frozen 1:25-1:100, Immunohistochemistry-Paraffin 1:1000, Immunoprecipitation 1:10-1:500, Western Blot 1:500-1:1000
Protocol Western blot Protocol for Ogg1 Antibody Western Blot Procedure
1. Run 50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/3 % BSA for 1 hour at room temperature (23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with NB100-106 (anti-hOGG1), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with goat anti-rabbit IgG-HRP, diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences.Immunohistochemistry Protocol for Ogg1 Antibody Immunohistochemistry - FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (AA 1-100) (ABIN151034) anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 1-100) antibody
Western Blotting (WB) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (AA 1-100) (ABIN151034) anti-8-Oxoguanine DNA Glycosylase (OGG1) (AA 1-100) antibody (Image 2)
Western Blotting (WB) image for anti-OGG1 antibody (8-Oxoguanine DNA Glycosylase) (AA 1-100) (ABIN151034) 1, 5, 10 ng titration of human recombinant Ogg1 protein, detected by ABIN151034.
Product cited in: Roseborough, Gao, Chen et al.: "The mitochondrial K-ATP channel opener, diazoxide, prevents ischemia-reperfusion injury in the rabbit spinal cord." in: The American journal of pathology, Vol. 168, Issue 5, pp. 1443-51, 2006 (PubMed).

Chen, Yu, Zhu et al.: "The p53 pathway promotes efficient mitochondrial DNA base excision repair in colorectal cancer cells." in: Cancer research, Vol. 66, Issue 7, pp. 3485-94, 2006 (PubMed).

Rachek, Thornley, Grishko et al.: "Protection of INS-1 cells from free fatty acid-induced apoptosis by targeting hOGG1 to mitochondria." in: Diabetes, Vol. 55, Issue 4, pp. 1022-8, 2006 (PubMed).

Xydas, Kherani, Chang et al.: "beta(2)-Adrenergic stimulation attenuates left ventricular remodeling, decreases apoptosis, and improves calcium homeostasis in a rodent model of ischemic cardiomyopathy." in: The Journal of pharmacology and experimental therapeutics, Vol. 317, Issue 2, pp. 553-61, 2006 (PubMed).

Druzhyna, Musiyenko, Wilson et al.: "Cytokines induce nitric oxide-mediated mtDNA damage and apoptosis in oligodendrocytes. Protective role of targeting 8-oxoguanine glycosylase to mitochondria." in: The Journal of biological chemistry, Vol. 280, Issue 22, pp. 21673-9, 2005 (PubMed).

Hashiguchi, Stuart, de Souza-Pinto et al.: "The C-terminal alphaO helix of human Ogg1 is essential for 8-oxoguanine DNA glycosylase activity: the mitochondrial beta-Ogg1 lacks this domain and does not have glycosylase activity." in: Nucleic acids research, Vol. 32, Issue 18, pp. 5596-608, 2004 (PubMed).

Stuart, Hashiguchi, Wilson et al.: "DNA base excision repair activities and pathway function in mitochondrial and cellular lysates from cells lacking mitochondrial DNA." in: Nucleic acids research, Vol. 32, Issue 7, pp. 2181-92, 2004 (PubMed).

Tuo, Chen, Zeng et al.: "Functional crosstalk between hOgg1 and the helicase domain of Cockayne syndrome group B protein." in: DNA repair, Vol. 1, Issue 11, pp. 913-27, 2003 (PubMed).

Rachek, Grishko, Musiyenko et al.: "Conditional targeting of the DNA repair enzyme hOGG1 into mitochondria." in: The Journal of biological chemistry, Vol. 277, Issue 47, pp. 44932-7, 2002 (PubMed).

Dobson, Grishko, LeDoux et al.: "Enhanced mtDNA repair capacity protects pulmonary artery endothelial cells from oxidant-mediated death." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 283, Issue 1, pp. L205-10, 2002 (PubMed).

Tyrberg, Anachkov, Dib et al.: "Islet expression of the DNA repair enzyme 8-oxoguanosine DNA glycosylase (Ogg1) in human type 2 diabetes." in: BMC endocrine disorders, Vol. 2, Issue 1, pp. 2, 2002 (PubMed).

Lin, Cao, Yu et al.: "Up-regulation of base excision repair activity for 8-hydroxy-2'-deoxyguanosine in the mouse brain after forebrain ischemia-reperfusion." in: Journal of neurochemistry, Vol. 74, Issue 3, pp. 1098-105, 2000 (PubMed).

Background publications Potts, Watkin, Hart: "Cadmium exposure down-regulates 8-oxoguanine DNA glycosylase expression in rat lung and alveolar epithelial cells." in: Toxicology, Vol. 184, Issue 2-3, pp. 189-202, 2002 (PubMed).

Mambo, Nyaga, Bohr et al.: "Defective repair of 8-hydroxyguanine in mitochondria of MCF-7 and MDA-MB-468 human breast cancer cell lines." in: Cancer research, Vol. 62, Issue 5, pp. 1349-55, 2002 (PubMed).

Fan, Liu, Huang et al.: "Frequent allelic imbalance and loss of protein expression of the DNA repair gene hOGG1 in head and neck squamous cell carcinoma." in: Laboratory investigation, a journal of technical methods and pathology, Vol. 81, Issue 10, pp. 1429-38, 2001 (PubMed).

Catalog No. ABIN151034

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