Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 496-789) antibody

Details for Product No. ABIN151055
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Antigen
Synonyms ARNT, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2, D3Ertd557e, Drnt, ESTM42, Hif1b, W08714, mKIAA4051, Arnt1
Epitope
AA 496-789
(13), (9), (5), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Ferret, Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)
(85), (42), (37), (21), (13), (12), (9), (9), (1), (1)
Host
Mouse
(52), (33), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(73), (28), (24), (21), (12), (10), (9), (5), (4), (1), (1)
Pubmed 12 references available
Quantity 0.1 mL
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Catalog No. ABIN151055
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Immunogen Fusion protein containing amino acids 496-789 of human HIF-1 beta.
Clone H1beta234
Isotype IgG1 kappa
Purification Protein G purified
Alternative Name HIF1 beta / ARNT
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease, and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) geneproducts. Alternate Names: anti-ARNT antibody, anti-Aryl Hydrocarbon Receptor Nuclear Translocator antibody,anti-Dioxin Receptor antibody, anti-Hypoxia Inducible Factor 1 antibody, anti-Tangoantibody, anti-HIF1 beta antibody, anti-HIF1beta antibody.
Gene Symbol: ARNT
Gene ID 405, 11863, 25242
UniProt P27540
Research Area Transcription Factors
Application Notes This HIF-1 beta (H1beta234) antibody is useful for Western Blot, Immunohistochemistry on paraffin-embedded sections and Chromatin Immunoprecipitation. By Western blot a band at approximately 92 kDa is seen. Use in Gel Shift Super Assay reported in scientific literature (PMID: 11325839).
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Gel Super Shift Assays, Immunohistochemistry 1:100, Immunohistochemistry-Paraffin 1:100, Immunoprecipitation, Western Blot 1:500
Protocol Protocol specific for HIF-1 beta Antibody Western Blot Procedure
1. Resolve aliquots (15 mg) of induced nuclear protein extracts on a SDS/6 % polyacrylamide gel.
. Transfer to nitrocellulose membranes in 20 mM Tris-HCl (pH 8.0)/150 mM glycine/20 % (vol/vol) methanol.
. Block membranes for 1.5 hours with 1X western wash buffer containing 5 % non-fat dry milk (NFDM).
. Incubate membranes for 1.5 hours at room temperature (RT) in NB 100-124 diluted 1:1,500 in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Incubate membranes with HRP conjugated anti-Mouse IgG for 1 hour (RT) in 1X western wash/5 % NFDM.
. Wash with 1X western wash for 35 minutes at RT (1 X 15 minutes, 2 X 10 minutes).
. Drain membrane and place on saran wrap.
. Using Amersham ECL Kit, mix equal volumes of two reagents. Pour over membrane (protein side facing up). Let solution sit on membrane for 15-20 seconds.
. Drain membrane and place on new saran wrap
. Wrap up membrane and expose to film.
. Develop accordingly.10X Western wash 24.2g Tris80g NaCl Tween-20 to 1 % pH 7.6 and QS to 4LStripping buffer100 mM BME2 % SDS62.5 mM Tris (pH 6.7)Incubate membrane for 30 minutes at 56 degrees C. Wash membrane 15 minutes with several changes of 1X western wash.
Restrictions For Research Use only
Format Liquid
Concentration 1.4 mg/mL
Buffer 1 X PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) (AA 496-789) antibody Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta (ABIN151055).
General Bernaudin, Tang, Reilly et al.: "Brain genomic response following hypoxia and re-oxygenation in the neonatal rat. Identification of genes that might contribute to hypoxia-induced ischemic tolerance." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39728-38, 2002 (PubMed).

Elvert, Kappel, Heidenreich et al.: "Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1)." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7520-30, 2003 (PubMed).

Mabjeesh, Willard, Harris et al.: "Dibenzoylmethane, a natural dietary compound, induces HIF-1 alpha and increases expression of VEGF." in: Biochemical and biophysical research communications, Vol. 303, Issue 1, pp. 279-86, 2003 (PubMed).

Mabjeesh, Escuin, LaVallee et al.: "2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF." in: Cancer cell, Vol. 3, Issue 4, pp. 363-75, 2003 (PubMed).

Kasuno, Takabuchi, Fukuda et al.: "Nitric oxide induces hypoxia-inducible factor 1 activation that is dependent on MAPK and phosphatidylinositol 3-kinase signaling." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2550-8, 2004 (PubMed).

Alam, Maizels, Park et al.: "Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follic" in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 19431-40, 2004 (PubMed).

Arsham, Plas, Thompson et al.: "Akt and hypoxia-inducible factor-1 independently enhance tumor growth and angiogenesis." in: Cancer research, Vol. 64, Issue 10, pp. 3500-7, 2004 (PubMed).

Hirota, Fukuda, Takabuchi et al.: "Induction of hypoxia-inducible factor 1 activity by muscarinic acetylcholine receptor signaling." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41521-8, 2004 (PubMed).

Amir, Wang, Matzkin et al.: "MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis." in: Cancer research, Vol. 66, Issue 2, pp. 856-66, 2006 (PubMed).

Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).

Nakamura, Martin, Jackson et al.: "Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells." in: Cancer research, Vol. 66, Issue 8, pp. 4249-55, 2006 (PubMed).

Asikainen, Chang, Coalson et al.: "Improved lung growth and function through hypoxia-inducible factor in primate chronic lung disease of prematurity." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1698-700, 2006 (PubMed).

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