Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT) antibody

Antigen: Aryl Hydrocarbon Receptor Nuclear Translocator (ARNT)

Synonyms: HIF1B, TANGO, bHLHe2, HIF1BETA, HIF-1beta, Drnt, Hif1b, ESTM42, W08714, KIAA4051, mKIAA4051, D3Ertd557e, Arnt1, ARNT, MGC136664, zgc:136664, arnt1, hif1b, tango, xArnt, hif1beta, hif-1beta, AHA-1

Clonality: Monoclonal (H1beta234)

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN151055

Additional Information

Alternative name: HIF1 beta / ARNT

Gene ID: 405, 11863, 25242

UniProt: P27540

Immunogen: Fusion protein containing amino acids 496-789 of human HIF-1 beta.

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine)

Isotype: IgG1

Clone: H1beta234

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease, and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) geneproducts. Alternate Names: anti-ARNT antibody, anti-Aryl Hydrocarbon Receptor Nuclear Translocator antibody,anti-Dioxin Receptor antibody, anti-Hypoxia Inducible Factor 1 antibody, anti-Tangoantibody, anti-HIF1 beta antibody, anti-HIF1beta antibody.

Specificity: This antibody is specific for HIF 1 beta /ARNT. Species Reactivity: Human, bovine, sheep, mouse, rat and ferret. Other species have not been tested.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:500,Immunohistochemistry-Paraffin 1:100

Concentration: 1.4 mg/ml

Purification: Purified

Buffer: PBS. Preservative: 0.05% sodium azide

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Transcription Factors

Restrictions: For Research Use only

Images

Immunohistochemical staining of human glioblastoma multi-forme utilizing anti-HIF-1 Beta (ABIN151055).

Publications

Bernaudin, Tang, Reilly et al.: "Brain genomic response following hypoxia and re-oxygenation in the neonatal rat. Identification of genes that might contribute to hypoxia-induced ischemic tolerance." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39728-38, 2002 (PubMed).

Elvert, Kappel, Heidenreich et al.: "Cooperative interaction of hypoxia-inducible factor-2alpha (HIF-2alpha ) and Ets-1 in the transcriptional activation of vascular endothelial growth factor receptor-2 (Flk-1)." in: The Journal of biological chemistry, Vol. 278, Issue 9, pp. 7520-30, 2003 (PubMed).

Mabjeesh, Willard, Harris et al.: "Dibenzoylmethane, a natural dietary compound, induces HIF-1 alpha and increases expression of VEGF." in: Biochemical and biophysical research communications, Vol. 303, Issue 1, pp. 279-86, 2003 (PubMed).

Mabjeesh, Escuin, LaVallee et al.: "2ME2 inhibits tumor growth and angiogenesis by disrupting microtubules and dysregulating HIF." in: Cancer cell, Vol. 3, Issue 4, pp. 363-75, 2003 (PubMed).

Kasuno, Takabuchi, Fukuda et al.: "Nitric oxide induces hypoxia-inducible factor 1 activation that is dependent on MAPK and phosphatidylinositol 3-kinase signaling." in: The Journal of biological chemistry, Vol. 279, Issue 4, pp. 2550-8, 2004 (PubMed).

Alam, Maizels, Park et al.: "Follicle-stimulating hormone activation of hypoxia-inducible factor-1 by the phosphatidylinositol 3-kinase/AKT/Ras homolog enriched in brain (Rheb)/mammalian target of rapamycin (mTOR) pathway is necessary for induction of select protein markers of follic" in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 19431-40, 2004 (PubMed).

Arsham, Plas, Thompson et al.: "Akt and hypoxia-inducible factor-1 independently enhance tumor growth and angiogenesis." in: Cancer research, Vol. 64, Issue 10, pp. 3500-7, 2004 (PubMed).

Hirota, Fukuda, Takabuchi et al.: "Induction of hypoxia-inducible factor 1 activity by muscarinic acetylcholine receptor signaling." in: The Journal of biological chemistry, Vol. 279, Issue 40, pp. 41521-8, 2004 (PubMed).

Amir, Wang, Matzkin et al.: "MSF-A interacts with hypoxia-inducible factor-1alpha and augments hypoxia-inducible factor transcriptional activation to affect tumorigenicity and angiogenesis." in: Cancer research, Vol. 66, Issue 2, pp. 856-66, 2006 (PubMed).

Hui, Bauer, Striet et al.: "Calcium signaling stimulates translation of HIF-alpha during hypoxia." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 3, pp. 466-75, 2006 (PubMed).

Nakamura, Martin, Jackson et al.: "Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells." in: Cancer research, Vol. 66, Issue 8, pp. 4249-55, 2006 (PubMed).

Asikainen, Chang, Coalson et al.: "Improved lung growth and function through hypoxia-inducible factor in primate chronic lung disease of prematurity." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 10, pp. 1698-700, 2006 (PubMed).