Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

Antigen: Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)

Synonyms: HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN151066

Additional Information

Alternative name: HIF1A / HIF1 alpha

Gene ID: 3091, 15251, 29560

UniProt: Q16665

Immunogen: Fusion protein containing amino acids 432-528 of human HIF-1alpha.

Cross-Reactivity: Human, Rat (Rattus)

Format: Serum

Description: Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease, and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting proteinantibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.

Specificity: This antibody is specific for HIF-1 alpha. Species Reactivity: Human, mouse and rat. Mouse results mixed. Other species have not been tested. Localization: Nucleus.

Application Details

Application Notes: Suggested working dilutions: chromatin immunoprecipitation - use 25 ul antibody for 15 million cells (nuclear extractsrecommended),immunohistochemistry 1:100,Western Blot 1:500-1:1000. Positive Controls: PC12 nuclear extracts (hypoxically induced) and Cobalt Chloride treated Cos-7 nuclearextracts

Purification: Serum

Buffer: Preservative: 0.05% Sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Western Blot analysis of ABIN151066, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates.

Staining of human kidney, renal tubular epithelium in Cortex using ABIN151066.

Publications

Bergeron, Gidday, Yu et al.: "Role of hypoxia-inducible factor-1 in hypoxia-induced ischemic tolerance in neonatal rat brain." in: Annals of neurology, Vol. 48, Issue 3, pp. 285-96, 2000 (PubMed).

Liu, Wu, Cai et al.: "Hypoxic preconditioning of cardiomyocytes and cardioprotection: phophorylation of HIF-1alpha induced by p42/p44 mitogen-activated protein kinases is involved." in: Pathophysiology : the official journal of the International Society for Pathophysiology / ISP, Vol. 9, Issue 4, pp. 201-205, 2003 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Harris, Brown, Jorgensen et al.: "Bone marrow-derived cells home to and regenerate retinal pigment epithelium after injury." in: Investigative ophthalmology & visual science, Vol. 47, Issue 5, pp. 2108-13, 2006 (PubMed).

Wang, Biju, Wang et al.: "Cytoprotective effects of hypoxia against cisplatin-induced tubular cell apoptosis: involvement of mitochondrial inhibition and p53 suppression." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 1875-85, 2006 (PubMed).

Lin, Lee, Yun: "Differentiation arrest by hypoxia." in: The Journal of biological chemistry, Vol. 281, Issue 41, pp. 30678-83, 2006 (PubMed).

Liao, Corle, Seagroves et al.: "Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression." in: Cancer research, Vol. 67, Issue 2, pp. 563-72, 2007 (PubMed).

Harvey, Kind, Thompson: "Regulation of gene expression in bovine blastocysts in response to oxygen and the iron chelator desferrioxamine." in: Biology of reproduction, Vol. 77, Issue 1, pp. 93-101, 2007 (PubMed).

Young, Williams, Chandra et al.: "Central role of Muc5ac expression in mucous metaplasia and its regulation by conserved 5' elements." in: American journal of respiratory cell and molecular biology, Vol. 37, Issue 3, pp. 273-90, 2007 (PubMed).

Kim, Gao, Liu et al.: "Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1." in: Molecular and cellular biology, Vol. 27, Issue 21, pp. 7381-93, 2007 (PubMed).

Ohtomo, Nangaku, Izuhara et al.: "Cobalt ameliorates renal injury in an obese, hypertensive type 2 diabetes rat model." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 23, Issue 4, pp. 1166-72, 2008 (PubMed).

Sikder, Kodadek: "The neurohormone orexin stimulates hypoxia-inducible factor-1 activity." in: Genes & development, Vol. 21, Issue 22, pp. 2995-3005, 2007 (PubMed).

Piantadosi, Suliman: "Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells." in: The Journal of biological chemistry, Vol. 283, Issue 16, pp. 10967-77, 2008 (PubMed).

Singh, Gu, Agarwal: "Silibinin inhibits colorectal cancer growth by inhibiting tumor cell proliferation and angiogenesis." in: Cancer research, Vol. 68, Issue 6, pp. 2043-50, 2008 (PubMed).

Lauzier, Robitaille, Chan et al.: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).