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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody
|Synonyms||HIF1, MOP1, PASD8, bHLHe78, HIF-1alpha, HIF1-ALPHA, HIF1A, HIF-1a, HIF1a, hif-1a, hif1a|
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|15 references available|
|Quantity||0.1 ml (Variants)|
|Price||Product not available in this region.|
|Alternative name||HIF1A / HIF1 alpha|
|Gene ID||3091, 15251, 29560|
|Immunogen||Fusion protein containing amino acids 432-528 of human HIF-1alpha.|
|Cross-Reactivity||Human, Rat (Rattus)|
|Description||Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease, and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting proteinantibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.|
|Specificity||This antibody is specific for HIF-1 alpha. Species Reactivity: Human, mouse and rat. Mouse results mixed. Other species have not been tested. Localization: Nucleus.|
|Application Notes||Suggested working dilutions: chromatin immunoprecipitation - use 25 ul antibody for 15 million cells (nuclear extractsrecommended),immunohistochemistry 1:100,Western Blot 1:500-1:1000. Positive Controls: PC12 nuclear extracts (hypoxically induced) and Cobalt Chloride treated Cos-7 nuclearextracts|
|Buffer||Preservative: 0.05% Sodium azide.|
|Storage||Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
|Western Blot analysis of ABIN151066, anti-HIF-1 alpha, on normoxic and hypoxic nuclear rat cell lysates. Staining of human kidney, renal tubular epithelium in Cortex using ABIN151066.|
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Lauzier, Robitaille, Chan et al.: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).