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Details for Product No. ABIN151067

Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) antibody

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Synonyms HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78, hif1a, HIF1-alpha, HIF-1a, HIF1a, hif-1a
»Alternatives Guinea Pig, Human, Monkey, Mouse (Murine), Rat (Rattus)
»Alternatives Rabbit
Clonality Polyclonal
»Alternatives Un-conjugated
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility-Shift Assay (EMSA), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 15 references available
Catalog no. ABIN151067
Quantity 25 µL
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Immunogen Fusion protein containing amino acids 432-528 of human HIF-1 alpha.
Specificity This antibody is specific for HIF-1 alpha. Species Reactivity: Human, mouse and rat. Mouse results mixed. Other species have not been tested. Localization: Nucleus.
Cross-Reactivity Human, Rat (Rattus)
Purification Serum
Alternative Name HIF1A / HIF1 alpha
Background Hypoxia contributes significantly to the pathophysiology of major categories of humandisease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension,congenital heart disease, and chronic obstructive pulmonary disease.HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as aposttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composedof HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated.However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 orPro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degradedin proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIFdegradation and increases transcriptional activity. Therefore, the concentration of HIF-1alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition.HIF hydroxylases provide insight into hypoxic cell responses, which may be used to helpisolate therapeutic targets. Alternate Names: anti-Hypoxia-inducible factor 1 alpha antibody, anti-HIF1 alpha antibody, anti-ARNTinteracting proteinantibody, anti-Hif1a antibody, anti-ARNT interacting protein antibody,anti-HIF-1alpha antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxiainducible factor 1 alpha subunit basic helix antibody.
Gene ID 3091
Application Notes Suggested working dilutions: chromatin immunoprecipitation - use 25 ul antibody for 15 million cells (nuclear extractsrecommended),immunohistochemistry 1:100,Western Blot 1:500-1:1,000. Positive Controls: PC12 nuclear extracts (hypoxically induced), Cobalt Chloride treated Cos-7 nuclearextracts
Restrictions For Research Use only
Format Liquid
Buffer Preservative: 0.05% Sodium azide.
Preservative Sodium azide
Storage -20 °C
Bergeron, Gidday, Yu et al.: "Role of hypoxia-inducible factor-1 in hypoxia-induced ischemic tolerance in neonatal rat brain." in: Annals of neurology, Vol. 48, Issue 3, pp. 285-96, 2000 (PubMed).

Liu, Wu, Cai et al.: "Hypoxic preconditioning of cardiomyocytes and cardioprotection: phophorylation of HIF-1alpha induced by p42/p44 mitogen-activated protein kinases is involved." in: Pathophysiology : the official journal of the International Society for Pathophysiology / ISP, Vol. 9, Issue 4, pp. 201-205, 2003 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Harris, Brown, Jorgensen et al.: "Bone marrow-derived cells home to and regenerate retinal pigment epithelium after injury." in: Investigative ophthalmology & visual science, Vol. 47, Issue 5, pp. 2108-13, 2006 (PubMed).

Wang, Biju, Wang et al.: "Cytoprotective effects of hypoxia against cisplatin-induced tubular cell apoptosis: involvement of mitochondrial inhibition and p53 suppression." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 1875-85, 2006 (PubMed).

Lin, Lee, Yun: "Differentiation arrest by hypoxia." in: The Journal of biological chemistry, Vol. 281, Issue 41, pp. 30678-83, 2006 (PubMed).

Liao, Corle, Seagroves et al.: "Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression." in: Cancer research, Vol. 67, Issue 2, pp. 563-72, 2007 (PubMed).

Harvey, Kind, Thompson: "Regulation of gene expression in bovine blastocysts in response to oxygen and the iron chelator desferrioxamine." in: Biology of reproduction, Vol. 77, Issue 1, pp. 93-101, 2007 (PubMed).

Young, Williams, Chandra et al.: "Central role of Muc5ac expression in mucous metaplasia and its regulation by conserved 5' elements." in: American journal of respiratory cell and molecular biology, Vol. 37, Issue 3, pp. 273-90, 2007 (PubMed).

Kim, Gao, Liu et al.: "Hypoxia-inducible factor 1 and dysregulated c-Myc cooperatively induce vascular endothelial growth factor and metabolic switches hexokinase 2 and pyruvate dehydrogenase kinase 1." in: Molecular and cellular biology, Vol. 27, Issue 21, pp. 7381-93, 2007 (PubMed).

Ohtomo, Nangaku, Izuhara et al.: "Cobalt ameliorates renal injury in an obese, hypertensive type 2 diabetes rat model." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 23, Issue 4, pp. 1166-72, 2008 (PubMed).

Sikder, Kodadek: "The neurohormone orexin stimulates hypoxia-inducible factor-1 activity." in: Genes & development, Vol. 21, Issue 22, pp. 2995-3005, 2007 (PubMed).

Piantadosi, Suliman: "Transcriptional Regulation of SDHa flavoprotein by nuclear respiratory factor-1 prevents pseudo-hypoxia in aerobic cardiac cells." in: The Journal of biological chemistry, Vol. 283, Issue 16, pp. 10967-77, 2008 (PubMed).

Singh, Gu, Agarwal: "Silibinin inhibits colorectal cancer growth by inhibiting tumor cell proliferation and angiogenesis." in: Cancer research, Vol. 68, Issue 6, pp. 2043-50, 2008 (PubMed).

Lauzier, Robitaille, Chan et al.: "(2R)-[(4-Biphenylylsulfonyl)amino]-N-hydroxy-3-phenylpropionamide (BiPS), a matrix metalloprotease inhibitor, is a novel and potent activator of hypoxia-inducible factors." in: Molecular pharmacology, Vol. 74, Issue 1, pp. 282-8, 2008 (PubMed).

Alternatives for antigen "Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A)", type "Antibodies"
Hosts (179), (136), (2)
Reactivities (277), (120), (98), (78), (52), (50), (31), (28), (20), (19), (17), (4), (2), (1), (1), (1), (1), (1), (1)
Applications (258), (95), (76), (71), (57), (55), (51), (30), (19), (13), (11), (10), (9), (6), (2), (2), (1), (1)
Conjugates (33), (20), (19), (12), (12), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (36), (24), (16), (11), (8), (7), (7), (6), (6), (6), (6), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)