Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody

Details for Product No. ABIN151067, Supplier: Log in to see
Antigen
  • hif1a
  • AA959795
  • HIF1alpha
  • MOP1
  • bHLHe78
  • HIF-1A
  • HIF-1alpha
  • HIF1
  • HIF1-ALPHA
  • PASD8
  • HIF1-alpha
  • hif-1a
  • hypoxia inducible factor 1 alpha
  • hypoxia inducible factor 1, alpha subunit
  • hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • hypoxia-inducible factor 1 alpha
  • hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
  • Protein HIF-1
  • hif-1a
  • Hif1a
  • HIF1A
  • hif-1
Alternatives
anti-Human Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) antibody for Electrophoretic Mobility-Shift Assay
Epitope
Internal Region
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1
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Reactivity
Dog (Canine), Guinea Pig, Human, Mouse (Murine), Primate, Rat (Rattus), Xenopus laevis, Zebrafish
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Host
Rabbit
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2
Clonality
Polyclonal
Conjugate
Un-conjugated
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25
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6
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Application
Chromatin Immunoprecipitation (ChIP), Gel Shift (GS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen A fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [UniProt Q16665].
Purification Immunogen affinity purified
Alternative Name HIF-1 alpha (HIF1A Antibody Abstract)
Background Gene Symbol: HIF1A
Molecular Weight Theoretical MW: 93 kDa
Gene ID 3091
UniProt Q61221, O35800, Q16665
Pathways Positive Regulation of Peptide Hormone Secretion, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis, Tube Formation, Regulation of Carbohydrate Metabolic Process, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Application Notes Western Blot 1:500 - 1:1000, Simple Western 1:100, Chromatin Immunoprecipitation 25 μL/ 15 million cells, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:100 - 1:500, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Gel Super Shift Assays 1:1 - 1:100ChIP usage was reported in scientific literature (PMID: 22891341). ICC/IF usage was reported in scientific literature (PMID: 21270095, 21270095). In WB a band is observed approx. 120 kDa. IHC applications have been mixed on Mouse. For ChIP, nuclear extracts are recommended. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer ( pH 6.0) is recommended. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size: Wes/Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol specific for HIF-1 alpha antibody Western Blot Protocol
1. Perform SDS-PAGE on hypoxic samples to be analyzed, loading 30 µg of nuclear protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry Protocol for HIF-1 alpha antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Western Blot analysis of ABIN151066, anti-HIF-1 alpha, on normoxic and hypoxic nuclea...
Immunohistochemistry (IHC) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Staining of human kidney, renal tubular epithelium in Cortex using ABIN151066.
Simple Western (SimWes) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Simple Western: HIF-1 alpha Antibody [ABIN151066] - Simple Western lane view shows a ...
Immunoprecipitation (IP) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Immunoprecipitation: HIF-1 alpha Antibody [ABIN151066] - PC-3 Cell Lysates run with C...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Western Blot: HIF-1 alpha Antibody [ABIN151066] - Analysis of HIF-1 alpha in human Re...
Western Blotting (WB) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Western Blot: HIF-1 alpha Antibody [ABIN151066] - Analysis of HIF-1 alpha in overexpr...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ABIN151066] - IHC analysis of a ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ABIN151066] - Staining in the ca...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Immunohistochemistry-Paraffin: HIF-1 alpha Antibody [ABIN151066] - Analysis of HIF-1 ...
Immunofluorescence (IF) image for anti-Hypoxia Inducible Factor 1, alpha Subunit (Basic Helix-Loop-Helix Transcription Factor) (HIF1A) (Internal Region) antibody (ABIN151067) Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody [ABIN151066] - Analysis ...
Product cited in: Murakami, Wang, Kalhorn, Schraml, Rathmell, Tan, Nemenoff, Stenmark, Jiang, Reyland, Heasley, Hu: "Context-dependent role for chromatin remodeling component PBRM1/BAF180 in clear cell renal cell carcinoma." in: Oncogenesis, Vol. 6, Issue 1, pp. e287, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Mimura, Hirakawa, Kanki, Kushida, Nakaki, Suzuki, Tanaka, Aburatani, Nangaku: "Novel lnc RNA regulated by HIF-1 inhibits apoptotic cell death in the renal tubular epithelial cells under hypoxia." in: Physiological reports, Vol. 5, Issue 8, 2017 (PubMed). Method employed by authors: Chromatin Immunoprecipitation (ChIP) (Sample species: Human).

Zhang, Liu, Liu, Cheng, Wang, Shi, Ding, Chen, Chen: "Endothelial cells co-cultured with renal carcinoma cells significantly reduce RECK expression under chemical hypoxia." in: Cell biology international, Vol. 41, Issue 8, pp. 922-927, 2017 (PubMed). (Sample species: Human).

Li, Du, Yuan, Han, Dong, Chen, Wu, Zhang, Xu, Han, Zhang, Xia: "Hepatic hypoxia-inducible factors inhibit PPARα expression to exacerbate acetaminophen induced oxidative stress and hepatotoxicity." in: Free radical biology & medicine, Vol. 110, pp. 102-116, 2017 (PubMed).

de Jong, van Balkom, Gremmels, Verhaar: "Exosomes from hypoxic endothelial cells have increased collagen crosslinking activity through up-regulation of lysyl oxidase-like 2." in: Journal of cellular and molecular medicine, Vol. 20, Issue 2, pp. 342-50, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Song, Liu, Shi, Zhang, Zhang, Chen: "Bioconjugated Manganese Dioxide Nanoparticles Enhance Chemotherapy Response by Priming Tumor-Associated Macrophages toward M1-like Phenotype and Attenuating Tumor Hypoxia." in: ACS nano, Vol. 10, Issue 1, pp. 633-47, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Takahashi, Davy, Gardner, Mathews, Yamazaki, Allsopp: "Hypoxia Inducible Factor 1 Alpha Is Expressed in Germ Cells throughout the Murine Life Cycle." in: PLoS ONE, Vol. 11, Issue 5, pp. e0154309, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Semba, Takeda, Isagawa, Sugiura, Honda, Wake, Miyazawa, Yamaguchi, Miura, Jenkins, Choi, Kim, Asagiri, Cowburn, Abe, Soma, Koyama, Katoh, Sayama, Goda, Johnson, Manabe, Nagai, Komuro: "HIF-1α-PDK1 axis-induced active glycolysis plays an essential role in macrophage migratory capacity." in: Nature communications, Vol. 7, pp. 11635, 2016 (PubMed). (Sample species: Mouse (Murine)). Further details: Chromatin Immunoprecipitation

Spitzbarth, Lempp, Kegler, Ulrich, Kalkuhl, Deschl, Baumgärtner, Seehusen: "Immunohistochemical and transcriptome analyses indicate complex breakdown of axonal transport mechanisms in canine distemper leukoencephalitis." in: Brain and behavior, Vol. 6, Issue 7, pp. e00472, 2016 (PubMed). (Sample species: Dog (Canine)). Further details: Immunohistochemistry

Kowitzke, Cohrs, Leuschner, Koch, Synowitz, Mehdorn, Held-Feindt, Knerlich-Lukoschus: "Cellular Profiles and Molecular Mediators of Lesion Cascades in the Placode in Human Open Spinal Neural Tube Defects." in: Journal of neuropathology and experimental neurology, Vol. 75, Issue 9, pp. 827-42, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Sullivan, Peet, Whitelaw: "MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors." in: The FEBS journal, Vol. 283, Issue 18, pp. 3488-502, 2016 (PubMed). Further details: Western Blotting

Campillo, Jorba, Schaedel, Casals, Gozal, Farré, Almendros, Navajas: "A Novel Chip for Cyclic Stretch and Intermittent Hypoxia Cell Exposures Mimicking Obstructive Sleep Apnea." in: Frontiers in physiology, Vol. 7, pp. 319, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Hung, Chang, Huang, Yin, Hwang, Yang, Yang: "Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons." in: Molecular neurobiology, Vol. 53, Issue 2, pp. 793-809, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Mori, Yao, Learman, Kurozumi, Ishida, Ramakrishnan, Overmyer, Xue, Cawthorn, Reid, Taylor, Ning, Shah, MacDougald: "Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion." in: Scientific reports, Vol. 6, pp. 21520, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Mammadzada, Gudmundsson, Kvanta, André: "Differential hypoxic response of human choroidal and retinal endothelial cells proposes tissue heterogeneity of ocular angiogenesis." in: Acta ophthalmologica, Vol. 94, Issue 8, pp. 805-814, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Lin, Li, Xing, Hu, Liang, Han, Wang, Hawke, Wang, Zhang, Wei, Ma, Park, Zhou, Zhou, Hu, Zhou, Marks, Liang, Hung, Lin, Yang: "The LINK-A lncRNA activates normoxic HIF1α signalling in triple-negative breast cancer." in: Nature cell biology, Vol. 18, Issue 2, pp. 213-24, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Joyal, Sun, Gantner, Shao, Evans, Saba, Fredrick, Burnim, Kim, Patel, Juan, Hurst, Hatton, Cui, Pierce, Bherer, Aguilar, Powner, Vevis, Boisvert, Fu, Levy, Fruttiger, Packard, Rezende, Maranda et al.: "Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1. ..." in: Nature medicine, Vol. 22, Issue 4, pp. 439-45, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Regan Anderson, Ma, Raj, Cidlowski, Helle, Knutson, Krutilina, Seagroves, Lange: "Breast Tumor Kinase (Brk/PTK6) Is Induced by HIF, Glucocorticoid Receptor, and PELP1-Mediated Stress Signaling in Triple-Negative Breast Cancer." in: Cancer research, Vol. 76, Issue 6, pp. 1653-63, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Hoang, Kadonosono, Kuchimaru, Kizaka-Kondoh: "Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model." in: Cancer science, Vol. 107, Issue 8, pp. 1151-8, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Kushida, Nomura, Mimura, Fujita, Yamamoto, Nangaku, Aburatani: "Hypoxia-Inducible Factor-1α Activates the Transforming Growth Factor-β/SMAD3 Pathway in Kidney Tubular Epithelial Cells." in: American journal of nephrology, Vol. 44, Issue 4, pp. 276-285, 2016 (PubMed).

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