Aralkylamine N-Acetyltransferase (AANAT) antibody

Details for Product No. ABIN151068
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Synonyms DSPS, SNAT, AA-NAT, Nat4, Nat-2, Snat
(52), (33), (27), (6), (1), (1), (1), (1)
(4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
(40), (20), (9), (8), (6), (3), (2), (1)
Pubmed 2 references available
Quantity 0.1 mL
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Catalog No. ABIN151068
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Immunogen Human Serotonin N-acetyltransferase
Specificity This is specific for primate AANAT (3,4).
No Cross-Reactivity Cow (Bovine), Chicken, Rat (Rattus), Zebrafish
Purification affinity purified
Alternative Name Serotonin acetylase
Background Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatoninsynthesis. Hormonal melatonin has been implicated in sleep and circadian clockfunction, and acts asthe hormone of the night. Large changes in theabundance of AANAT are believed to be responsible for the large day/night rhythms inmelatonin synthesis in the pineal gland and retina (1,2).Most research shows that AANAT activity is regulated by controlling the steady-statelevels of the protein. Recent research, however, suggests that another mechanism mayalso play a role in controlling melatonin production without altering AANAT protein andactivity. This mechanism may explain the sudden physiological increase in circulatingmelatonin observed at the onset of the dark period in humans (3). Alternate Names: anti-Arylalkamine N-Acetyltransferase antibody, anti-Serotonin-N-Acetyltransferaseantibody, anti-SNAT antibody.
Gene Symbol: AANAT
Gene ID 15, 25120
UniProt Q16613
Research Area Enzymes
Application Notes This Serotonin N-acetyltransferase antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot, this antibody recognizes a band at 23 kDa.
Recommended dilutions: Immunocytochemistry/Immunofluorescence 1:200, Western Blot 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Buffer Eluted in 0.1N glacial acetic acid containing 0.1 % BSA & neutralized using 10x PBS, Thimerosal
Preservative Thimerosal (Merthiolate)
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
anti-Aralkylamine N-Acetyltransferase (AANAT) antibody AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde, 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.
anti-Aralkylamine N-Acetyltransferase (AANAT) antibody (2) Western blot analysis of hAANAT. Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity (~3.5 nmol of tryptamine acetylated per hour) in 65 ug and 2.6 ng of protein, respectively. A sample of a homogenized human pineal gland containing 33.5 ug of protein was loaded on a separate gel.
General Klein, Ganguly, Coon et al.: "14-3-3 proteins in pineal photoneuroendocrine transduction: how many roles?" in: Journal of neuroendocrinology, Vol. 15, Issue 4, pp. 370-7, 2003 (PubMed).

Iuvone, Brown, Haque et al.: "Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase." in: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 564-72, 2002 (PubMed).

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