Aralkylamine N-Acetyltransferase (AANAT) antibody

Antigen: Aralkylamine N-Acetyltransferase (AANAT)

Synonyms: SNAT, AA-NAT

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN151068

Additional Information

Alternative name: Serotonin acetylase

Gene ID: 15, 25120

UniProt: U40347

Immunogen: Human AANAT. Reference Sequence: U40347.

Cross-Reactivity: Human, Monkey

Description: Arylalkylamine N-Acetyltransferase (AANAT) is the penultimate enzyme is melatoninsynthesis. Hormonal melatonin has been implicated in sleep and circadian clockfunction, and acts asthe hormone of the night. Large changes in theabundance of AANAT are believed to be responsible for the large day/night rhythms inmelatonin synthesis in the pineal gland and retina (1,2).Most research shows that AANAT activity is regulated by controlling the steady-statelevels of the protein. Recent research, however, suggests that another mechanism mayalso play a role in controlling melatonin production without altering AANAT protein andactivity. This mechanism may explain the sudden physiological increase in circulatingmelatonin observed at the onset of the dark period in humans (3). Alternate Names: anti-Arylalkamine N-Acetyltransferase antibody, anti-Serotonin-N-Acetyltransferaseantibody, anti-SNAT antibody.

Specificity: This antibody is specific for primate AANAT (3,4). Species Reactivity: Recognizes human and rhesus AANAT. It does not recognize rat, ovine, chicken orzebrafish AANAT.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:500-1:1000,Immunocytochemistry 1:200

Purification: Purified

Buffer: Immunopurified rabbit antiserum (100 ul). The peptide (hAANAT1-26) was bound tostrips of PVDF membrane. The antiserum was eluted in 0.1N glacial acetic acidcontaining 0.1% BSA and neutralized using 10X PBS containing NaOH to a final pH of6.8.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Enzymes

Restrictions: For Research Use only

Images

AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde, 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.

Western blot analysis of hAANAT. Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity (~3.5 nmol of tryptamine acetylated per hour) in 65 ug and 2.6 ng of protein, respectively. A sample of a homogenized human pineal gland containing 33.5 ug of protein was loaded on a separate gel.

Publications

Iuvone, Brown, Haque et al.: "Retinal melatonin production: role of proteasomal proteolysis in circadian and photic control of arylalkylamine N-acetyltransferase." in: Investigative ophthalmology & visual science, Vol. 43, Issue 2, pp. 564-72, 2002 (PubMed).

Klein, Ganguly, Coon et al.: "14-3-3 proteins in pineal photoneuroendocrine transduction: how many roles?" in: Journal of neuroendocrinology, Vol. 15, Issue 4, pp. 370-7, 2003 (PubMed).