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Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

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(85), (27), (13), (2), (1), (1)
(63), (21), (2)
(5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(62), (35), (23), (18), (11), (6), (4), (3), (3), (2), (2), (1), (1)
Pubmed 14 references available
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Quantity 0.1 mL
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Immunogen The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Cross-Reactivity (Details) This antibody does not appear to work in Mouse. Results have been mixed in rat,with success in western analysis,immunofluorescence on rat,endothelial cells,negative results with PC12 cells. Expected reactivity with Rabbit, Orangutan, Rhesus Monkey, Gorilla based on 100% sequence identity.
Purification affinity purified
Alternative Name EGLN1 / PHD2 (EGLN1 Antibody Abstract)
Background HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis.HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame 12 antibody,anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1 antibody, anti-HIFProlyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2 antibody,anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody, anti-EGLN1antibody, anti-PHD2 antibody.
Gene Symbol: EGLN1
Gene ID 54583, 112405, 308913
NCBI Accession NP_071334
Application Notes This PHD2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplamic and nuclear staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature.
Recommended dilutions: Flow Cytometry 3.0 mcg/mL, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:10-1:2000, Immunoprecipitation, Western Blot 1:500-1:2500
Protocol Protocol specific for HIF Prolyl Hydroxylase 2 Antibody IHC-FFPE sections I. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-citrate/Phosphate, pH 7-8, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (ABIN151071) Western Blot analysis of human PHD2, using ABIN151071. Samples: Recombinant DYKDDDDK-...
Immunohistochemistry (IHC) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (ABIN151071) anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (Image 2)
Flow Cytometry (FACS) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (ABIN151071) anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (Image 3)
Product cited in: Aprelikova, Pandolfi, Tackett et al.: "Melanoma antigen-11 inhibits the hypoxia-inducible factor prolyl hydroxylase 2 and activates hypoxic response." in: Cancer research, Vol. 69, Issue 2, pp. 616-24, 2009 (PubMed).

Rantanen, Pursiheimo, Hoegel et al.: "Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 2231-40, 2008 (PubMed).

Barth, Nesper, Hasgall et al.: "The peptidyl prolyl cis/trans isomerase FKBP38 determines hypoxia-inducible transcription factor prolyl-4-hydroxylase PHD2 protein stability." in: Molecular and cellular biology, Vol. 27, Issue 10, pp. 3758-68, 2007 (PubMed).

Yin, Kharbanda, Kufe: "Mucin 1 oncoprotein blocks hypoxia-inducible factor 1alpha activation in a survival response to hypoxia." in: The Journal of biological chemistry, Vol. 282, Issue 1, pp. 257-66, 2007 (PubMed).

Li, Yi, Sundy et al.: "Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys." in: American journal of physiology. Renal physiology, Vol. 292, Issue 1, pp. F207-16, 2007 (PubMed).

McMahon, Charbonneau, Grandmont et al.: "Transforming growth factor beta1 induces hypoxia-inducible factor-1 stabilization through selective inhibition of PHD2 expression." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24171-81, 2006 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Elvidge, Glenny, Appelhoff et al.: "Concordant regulation of gene expression by hypoxia and 2-oxoglutarate-dependent dioxygenase inhibition: the role of HIF-1alpha, HIF-2alpha, and other pathways." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15215-26, 2006 (PubMed).

Jokilehto, Rantanen, Luukkaa et al.: "Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 4, pp. 1080-7, 2006 (PubMed).

Asikainen, Schneider, Waleh et al.: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).

Hirsilae, Koivunen, Xu et al.: "Effect of desferrioxamine and metals on the hydroxylases in the oxygen sensing pathway." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 10, pp. 1308-10, 2005 (PubMed).

Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Background publications Wu, Dudognon, Nguyen et al.: "Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths." in: Journal of cell science, Vol. 119, Issue Pt 13, pp. 2797-806, 2006 (PubMed).

Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).

Catalog No. ABIN151071

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