Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody

Antigen: Egl Nine Homolog 1 (C. Elegans) (EGLN1)

Synonyms: HPH2, PHD2, SM20, ECYT3, HIFPH2, ZMYND6, C1orf12, DKFZp761F179, Phd2, ORF13, SM-20, AI503754, zgc:92314, HPH-2, PHD-2, HIF-PH2, EGLN1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Catalog no.: ABIN151071

Additional Information

Alternative name: EGLN1 / PHD2

Gene ID: 54583, 112405, 308913

NCBI Acession: NP_0713341

Immunogen: The epitope recognized by this antibody maps to a region between residues 1 and 50 ofhuman Prolyl Hydroxylase Domain-Containing Protein 2 using the numbering given inentry NP_071334.1 (GeneID 54583).

Cross-Reactivity: Human

Description: HIF prolyl hydroxylase 2 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis.HPH-2, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(402) and Pro(564), and HIF-2 alpha. It targets HIF through the hydroxylation for proteasomal degredation via the vonHippel-Lindau ubiquitylation complex. Alternate Names: anti-C1ORF12 antibody, anti-Chromosome 1 Open Reading Frame 12 antibody,anti-EGL 9 homolog of C. elegans 1 antibody, anti-EGLN 1 EGLN1 antibody, anti-HIFProlyl Hydroxylase 2 antibody, anti-HIF-P4H 2 antibody, anti-HIFPH2 antibody,anti-P4H2 antibody, anti-Prolyl Hydroxylase Domain Containing antibody, anti-EGLN1antibody, anti-PHD2 antibody.

Specificity: This antibody is specific for human HIF prolyl hydroxylase 2. Species Reactivity: Human. This antibody does not appear to work in Mouse. Results have been mixed inRat with success in western analysis and immunofluorescence on Rat endothelial cellsand negative results with PC12 cells.

Application Details

Application Notes: Suggested working dilutions: flow cytometry / FACS analysis 3.0 mcg/ml,Western Blot 1:500-1:2500

Concentration: 1 mg/ml

Purification: Aff - Purified

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.09% Sodium Azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Western Blot analysis of human PHD2, using ABIN151071. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells.

anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (Image 2)

anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) antibody (Image 3)

Publications

Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).

Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Hirsilae, Koivunen, Xu et al.: "Effect of desferrioxamine and metals on the hydroxylases in the oxygen sensing pathway." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 19, Issue 10, pp. 1308-10, 2005 (PubMed).

Asikainen, Schneider, Waleh et al.: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).

Jokilehto, Rantanen, Luukkaa et al.: "Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 4, pp. 1080-7, 2006 (PubMed).

Elvidge, Glenny, Appelhoff et al.: "Concordant regulation of gene expression by hypoxia and 2-oxoglutarate-dependent dioxygenase inhibition: the role of HIF-1alpha, HIF-2alpha, and other pathways." in: The Journal of biological chemistry, Vol. 281, Issue 22, pp. 15215-26, 2006 (PubMed).

Ietta, Wu, Winter et al.: "Dynamic HIF1A regulation during human placental development." in: Biology of reproduction, Vol. 75, Issue 1, pp. 112-21, 2006 (PubMed).

Wu, Dudognon, Nguyen et al.: "Immunodetection of human telomerase reverse-transcriptase (hTERT) re-appraised: nucleolin and telomerase cross paths." in: Journal of cell science, Vol. 119, Issue Pt 13, pp. 2797-806, 2006 (PubMed).

McMahon, Charbonneau, Grandmont et al.: "Transforming growth factor beta1 induces hypoxia-inducible factor-1 stabilization through selective inhibition of PHD2 expression." in: The Journal of biological chemistry, Vol. 281, Issue 34, pp. 24171-81, 2006 (PubMed).

Li, Yi, Sundy et al.: "Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys." in: American journal of physiology. Renal physiology, Vol. 292, Issue 1, pp. F207-16, 2007 (PubMed).

Yin, Kharbanda, Kufe: "Mucin 1 oncoprotein blocks hypoxia-inducible factor 1alpha activation in a survival response to hypoxia." in: The Journal of biological chemistry, Vol. 282, Issue 1, pp. 257-66, 2007 (PubMed).

Barth, Nesper, Hasgall et al.: "The peptidyl prolyl cis/trans isomerase FKBP38 determines hypoxia-inducible transcription factor prolyl-4-hydroxylase PHD2 protein stability." in: Molecular and cellular biology, Vol. 27, Issue 10, pp. 3758-68, 2007 (PubMed).

Rantanen, Pursiheimo, Hoegel et al.: "Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 2231-40, 2008 (PubMed).

Aprelikova, Pandolfi, Tackett et al.: "Melanoma antigen-11 inhibits the hypoxia-inducible factor prolyl hydroxylase 2 and activates hypoxic response." in: Cancer research, Vol. 69, Issue 2, pp. 616-24, 2009 (PubMed).