Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody

Details for Product No. ABIN151071, Supplier: Log in to see
Antigen
  • EGLN1
  • egln2
  • phd2
  • zgc:92314
  • AI503754
  • C1orf12
  • HIF-PH2
  • HPH-2
  • Hif-p4h-2
  • ORF13
  • Phd2
  • SM-20
  • PHD-2
  • PHD2
  • ECYT3
  • HIFPH2
  • HPH2
  • SM20
  • ZMYND6
  • egl nine homolog 1 (C. elegans)
  • egl nine homolog 1
  • EGL nine homolog 1 (C. elegans)
  • egl-9 family hypoxia-inducible factor 1
  • EGLN1
  • egln1
  • Egln1
Alternatives
anti-Rat (Rattus) Egl Nine Homolog 1 (C. Elegans) antibody for Electron Microscopy
Epitope
AA 1-50
37
32
18
15
13
8
8
7
5
3
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
148
63
42
12
3
3
2
2
2
1
1
1
1
Host
Rabbit
119
34
2
Clonality
Polyclonal
Conjugate
Un-conjugated
12
10
8
8
8
7
5
5
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
126
79
59
48
34
34
27
13
11
2
1
1
1
1
1
Options
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Immunogen The epitope recognized by this antibody maps to a region between residues 1 and 50 of human PHD2/HIF Prolyl Hydroxylase 2 using the numbering given in entry NP_071334.1 (GeneID 54583).
Purification Immunogen affinity purified
Alternative Name EGLN1/PHD2 (EGLN1 Antibody Abstract)
Background Gene Symbol: EGLN1
Molecular Weight Theoretical MW: 46 kDa
Gene ID 54583
UniProt Q9GZT9
Pathways cAMP Metabolic Process
Application Notes Western Blot 1:500-1:2500, Simple Western 1:500, Flow Cytometry 3.0 mcg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation, Immunohistochemistry-Paraffin 1:10-1:500This PHD2 antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot, and Immunohistochemistry-paraffin embedded sections. In ICC/IF, cytoplamic and nuclear staining was observed in HeLa cells. Immunoprecipitation was reported in scientific literature. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for HIF Prolyl Hydroxylase 2 Antibody IHC-FFPE sections I. Deparaffinization: A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes. II. Quench Endogenous Peroxidase: A. Place slides in peroxidase quenching solution: 15-30 minutes. To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation B. Place slides in distilled water: 2 changes for 2 minutes each. III. Retrieve Epitopes: A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Western Blot analysis of human PHD2, using ABIN151071. Samples: Recombinant DYKDDDDK-...
Immunohistochemistry (IHC) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (Image 2)
Flow Cytometry (FACS) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (Image 3)
Simple Western (SimWes) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Simple Western: EGLN1/PHD2 Antibody [ABIN151071] - Simple Western lane view shows a s...
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Western Blot: EGLN1/PHD2 Antibody [ABIN151071] - Analysis of PHD2 in Hep3B cell lysate.
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Western Blot: EGLN1/PHD2 Antibody [ABIN151071] - Analysis of PHD2 in normoxia (lane 1...
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Western Blot: EGLN1/PHD2 Antibody [ABIN151071] - Detection of PHD2 human glioblastoma...
Western Blotting (WB) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Western Blot: EGLN1/PHD2 Antibody [ABIN151071] - Analysis of PHD2 expression in human...
Immunofluorescence (IF) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [ABIN151071] - Staining o...
Immunofluorescence (IF) image for anti-Egl Nine Homolog 1 (C. Elegans) (EGLN1) (AA 1-50) antibody (ABIN151071) Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody [ABIN151071] - Staining o...
Product cited in: Miles, Burr, Grice, Nathan: "The vacuolar-ATPase complex and assembly factors, TMEM199 and CCDC115, control HIF1α prolyl hydroxylation by regulating cellular iron levels." in: eLife, Vol. 6, 2017 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Tao, Lin, Li, Shen, Wang: "Prolyl hydroxylase-2 inhibits liver tumor cell proliferation and cyclin D1 expression in a hydroxylase-dependent manner." in: The international journal of biochemistry & cell biology, Vol. 77, Issue Pt A, pp. 129-40, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Mysore, Szablowski, Dervan, Frost: "A DNA-binding Molecule Targeting the Adaptive Hypoxic Response in Multiple Myeloma Has Potent Antitumor Activity." in: Molecular cancer research : MCR, Vol. 14, Issue 3, pp. 253-66, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Burr, Costa, Grice, Timms, Lobb, Freisinger, Dodd, Dougan, Lehner, Frezza, Nathan: "Mitochondrial Protein Lipoylation and the 2-Oxoglutarate Dehydrogenase Complex Controls HIF1α Stability in Aerobic Conditions." in: Cell metabolism, Vol. 24, Issue 5, pp. 740-752, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Caprara, Scappa, Garrafa, Di Castro, Rosanò, Bagnato, Spinella: "Endothelin-1 regulates hypoxia-inducible factor-1α and -2α stability through prolyl hydroxylase domain 2 inhibition in human lymphatic endothelial cells." in: Life sciences, Vol. 118, Issue 2, pp. 185-90, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Vogler, Zieseniss, Hesse, Levent, Tiburcy, Heinze, Burzlaff, Schley, Eckardt, Willam, Katschinski: "Pre- and post-conditional inhibition of prolyl-4-hydroxylase domain enzymes protects the heart from an ischemic insult." in: Pflügers Archiv : European journal of physiology, Vol. 467, Issue 10, pp. 2141-9, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Li, Yuan, Jiang, Ye, Yang, Shapiro, Risbud: "Prolyl-4-hydroxylase domain protein 2 controls NF-κB/p65 transactivation and enhances the catabolic effects of inflammatory cytokines on cells of the nucleus pulposus." in: The Journal of biological chemistry, Vol. 290, Issue 11, pp. 7195-207, 2015 (PubMed). (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence

Hsiao, Hsu, Liu, Hsieh, Chou, Wu, Jiang, Lai: "Deltex1 antagonizes HIF-1α and sustains the stability of regulatory T cells in vivo." in: Nature communications, Vol. 6, pp. 6353, 2015 (PubMed). Further details: Western Blotting

Kumar, Gullberg, Olsson, Ajore: "Myeloid translocation gene-16 co-repressor promotes degradation of hypoxia-inducible factor 1." in: PLoS ONE, Vol. 10, Issue 5, pp. e0123725, 2015 (PubMed). Further details: Western Blotting,Immunoprecipitation

Ryou, Choudhury, Li, Winters, Yuan, Liu, Yang: "Methylene blue-induced neuronal protective mechanism against hypoxia-reoxygenation stress." in: Neuroscience, Vol. 301, pp. 193-203, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Högel, Miikkulainen, Bino, Jaakkola: "Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27." in: Molecular cancer, Vol. 14, pp. 143, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Sun, Kosyna, Jelkmann, Depping: "Prolyl-4-Hydroxylase 2 Potentially Contributes to Hepatocellular Carcinoma-Associated Erythrocytosis by Maintaining Hepatocyte Nuclear Factor-4α Expression." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 37, Issue 6, pp. 2257-64, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhang, Pang, Tang, Yin, Wang: "Overexpression of hypoxia-inducible factor prolyl hydoxylase-2 attenuates hypoxia-induced vascular endothelial growth factor expression in luteal cells." in: Molecular medicine reports, Vol. 12, Issue 3, pp. 3809-14, 2015 (PubMed).

Rawluszko, Bujnicka, Horbacka, Krokowicz, Jagodziński: "Expression and DNA methylation levels of prolyl hydroxylases PHD1, PHD2, PHD3 and asparaginyl hydroxylase FIH in colorectal cancer." in: BMC cancer, Vol. 13, pp. 526, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Lindholm, Fischer, Poellinger, Johnson, Gustafsson, Sundberg, Rundqvist: "Negative regulation of HIF in skeletal muscle of elite endurance athletes: a tentative mechanism promoting oxidative metabolism." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 307, Issue 3, pp. R248-55, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Sun, Jelkmann, Depping: "Prolyl-4-hydroxylase 2 enhances hypoxia-induced glioblastoma cell death by regulating the gene expression of hypoxia-inducible factor-α." in: Cell death & disease, Vol. 5, pp. e1322, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Botting, McMillen, Forbes, Nyengaard, Morrison: "Chronic hypoxemia in late gestation decreases cardiomyocyte number but does not change expression of hypoxia-responsive genes." in: Journal of the American Heart Association, Vol. 3, Issue 4, 2014 (PubMed). Further details: Western Blotting

Tan, Prosser, Vanags, Monger, Ng, Bursill: "High-density lipoproteins augment hypoxia-induced angiogenesis via regulation of post-translational modulation of hypoxia-inducible factor 1α." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, Issue 1, pp. 206-17, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Henze, Garvalov, Seidel, Cuesta, Ritter, Filatova, Foss, Dopeso, Essmann, Maxwell, Reifenberger, Carmeliet, Acker-Palmer, Acker: "Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR." in: Nature communications, Vol. 5, pp. 5582, 2014 (PubMed).

Verstraete, Debucquoy, Devos, Sagaert, Penninckx, Begg, Haustermans: "Investigation of possible endogenous hypoxia markers in colorectal cancer." in: International journal of radiation biology, Vol. 89, Issue 1, pp. 9-15, 2013 (PubMed). (Sample species: Human). Further details: Immunohistochemistry