Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody

Details for Product No. ABIN151073
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Antigen
Synonyms HIFP4H3, HIFPH3, PHD3, 2610021G09Rik, AI505553, AI648162, Hif-p4h-3, Phd3, SM-20, phd3, PHD-3, zgc:77019, wu:fj78a08, EGLN3
Reactivity
Human, Rat (Rattus)
(51), (23), (23), (17), (14), (13), (6), (1), (1)
Host
Rabbit
(36), (14), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(43), (12), (10), (10), (5), (2), (1), (1), (1)
Pubmed 10 references available
Catalog no. ABIN151073
Quantity 0.1 mL
Price
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Immunogen The epitope recognized by this antibody maps to a region between residue 50 and 100of human Prolyl Hydroxylase Domain-Containing Protein 3 using the numbering given inentry NP_071356.1 (GeneID 112399).
Specificity This antibody is specific for HIF prolyl hydroxylase 3. Species Reactivity: Human and Rat. Does not appear to work in Mouse.
Cross-Reactivity Human
Purification Affinity Purified
Alternative Name EGLN3 / PHD3
Background HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF-alpha. Classic prolylhydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIFis an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH isan essential component of the pathway through which cells sense oxygen. In thepresence of oxygen, HPHs convert specific prolyl residues in HIF-alpha tohydroxyproline, leading to HIF-alpha destruction. Low oxygen levels, sensed at thecellular level, cause the HIF conversion to be reduced so that HIF is stable and there isincreased angiogenesis.HPH-1, specifically, catalyzes the posttranslational formation of 4-hydroxyproline in HIFalpha proteins. It hydroxylates HIF-1 alpha at Pro(564) and HIF-2 alpha. It targets HIFthrough the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may also play a role in cell growth regulation in muscle cellsand in apoptosis in neuronal tissues, promoting cell death through a caspase-dependentmechanism. Alternate Names: anti-EGL9 homolog of C. elegans 3 antibody, anti-EGLN3 antibody, anti-Factorresponsive smooth muscle protein antibody, anti-HIF Prolyl Hydroxylase 3 antibody,anti-HIFP4H3 antibody, anti-HIFPH3 antibody, anti-P4H3 antibody, anti-ProlylHydroxylase Domain Containing Protein 3 antibody, anti-SM20 antibody, anti-EGLN3antibody, anti-PHD3 antibody.
Gene ID 112399, 112407, 54702
NCBI Accession NP_0713561
Application Notes This antibody may be used for Western blotting where a band is seen at ~27 kDa,representing HIF prolyl hydroxylase 3. This antibody has been tested in 293 cell lysates.Suggested working dilutions: *Western Blot 1:1,000-1:5000, Immunohistochemistry ND for this lotImmunoprecipitation ND for this lot
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/ml
Buffer Tris-citrate/phosphate buffer, pH 7 to 8. Preservative: 0.1% Sodium Azide.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Egl Nine Homolog 3 (C. Elegans) (EGLN3) antibody Detection of Human and Rat PHD3 by Western Blot. Samples: A. Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. B. Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3 (ABIN151073) use at 1:1,000 (A) or 1:5,000 (B). Detection:Chemiluminence.
General Bruick, McKnight: "A conserved family of prolyl-4-hydroxylases that modify HIF." in: Science (New York, N.Y.), Vol. 294, Issue 5545, pp. 1337-40, 2001 (PubMed).

Ivan, Haberberger, Gervasi et al.: "Biochemical purification and pharmacological inhibition of a mammalian prolyl hydroxylase acting on hypoxia-inducible factor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 21, pp. 13459-64, 2002 (PubMed).

Asikainen, Schneider, Waleh et al.: "Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 29, pp. 10212-7, 2005 (PubMed).

Jokilehto, Rantanen, Luukkaa et al.: "Overexpression and nuclear translocation of hypoxia-inducible factor prolyl hydroxylase PHD2 in head and neck squamous cell carcinoma is associated with tumor aggressiveness." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 12, Issue 4, pp. 1080-7, 2006 (PubMed).

Asikainen, Waleh, Schneider et al.: "Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 291, Issue 4, pp. L588-95, 2006 (PubMed).

Li, Yi, Sundy et al.: "Expression and actions of HIF prolyl-4-hydroxylase in the rat kidneys." in: American journal of physiology. Renal physiology, Vol. 292, Issue 1, pp. F207-16, 2007 (PubMed).

Fu, Menzies, Freeman et al.: "EGLN3 prolyl hydroxylase regulates skeletal muscle differentiation and myogenin protein stability." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12410-8, 2007 (PubMed).

Bai, Zeng, Hu et al.: "Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants." in: Biotechnology letters, Vol. 29, Issue 12, pp. 2007-12, 2007 (PubMed).

Rantanen, Pursiheimo, Hoegel et al.: "Prolyl hydroxylase PHD3 activates oxygen-dependent protein aggregation." in: Molecular biology of the cell, Vol. 19, Issue 5, pp. 2231-40, 2008 (PubMed).

Ginouvès, Ilc, Macías et al.: "PHDs overactivation during chronic hypoxia \desensitizes\" HIFalpha and protects cells from necrosis."" in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 12, pp. 4745-50, 2008 (PubMed).

Hosts (36), (14), (5)
Reactivities (51), (23), (23), (17), (14), (13), (6), (1), (1)
Applications (43), (12), (10), (10), (5), (2), (1), (1), (1)
Conjugates (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (7), (5), (3), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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