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MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody

Details for Product No. ABIN151076, Supplier: Log in to see
Antigen
  • wu:faa63d12
  • zgc:64018
  • Mre11
  • Mre11b
  • ATLD
  • HNGS1
  • MRE11
  • MRE11B
  • meiotic recombination 11 homolog A (S. cerevisiae)
  • MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
  • mre11a
  • MRE11A
  • Mre11a
Alternatives
anti-Human MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) antibody for Western Blotting
Reactivity
Chicken, Hamster, Human, Mouse (Murine), Rat (Rattus)
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106
82
8
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4
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
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38
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
146
78
60
45
39
26
22
19
5
4
3
3
3
3
1
1
1
Options
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Immunogen Full length human Mre11 protein. [UniProt# P49959]
Purification Unpurified
Alternative Name Mre11 (MRE11A Antibody Abstract)
Background Gene Symbol: MRE11A
Molecular Weight Theoretical MW: 81 kDa
Gene ID 4361
UniProt P49959
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Pathways DNA Damage Repair
Application Notes Western Blot 1:5000, Simple Western 1:250, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 3 μL, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-FrozenThis Mre11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections (PMID 21279473) Immunoprecipitation and Western Blot. In WB, a band can be seen at approx. 81 kDa. For ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. For IP, the suggested working dilution is 3 μL for immunoprecipitation of 3X10^6 cells. Co-IP application has been reported by Ching et al 2012 (PMID: 22190719). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24349281) In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Mre11 Antibody Protocol specific for Mre11 Antibody Immunoprecipitation Procedure
1. For IP reactions, start with extract (whole cell or nuclear) from around 3 million cells prepared in 0.5-1 mL lysis buffer (100 mM NaCl, 10 mM Tris HCl, 5 mM EDTA, 0.5 % nonidet p40).
. Cells are resuspended in lysis buffer, then incubated with rotation about 15 min at 4 degrees C.
. The lysate is then centrifuged 5 min at 14000g to remove insoluble material.
. To cleared lysate, add 1-3 µL of antiserum and incubate on ice for 30 min.
. Collect immune complexes on Protein A Sepharose by adding 25 µL of a 50 % slurry, and incubate with rotation for 1 hour at 4 degrees C.
. The complexes are pelleted gently (5000g for 5-10 sec.) then washed with 1 mL lysis buffer.
. Repeat the wash 2 more times.
. Analyze the immunoprecipitates by SDS PAGE. This antibody works well for IP reactions from both human and mouse cells. The intact complex is stable and can be immunoprecipitated in many common lysis buffers (up to 0.5 M NaCl). Western Blot Procedure
. Run 50 µg of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/3 % BSA for 1 hour at room temperature (23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with 1:5000 dilution of NB100-142 (anti-hMre11), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with goat anti-rabbit IgG-HRP, diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. NOTE: HeLa whole cell extracts were used as a positive control for this antibody.Immunofluorescence Procedure A 5beta in situ extraction method [10mM Pipes, pH 6.8 / 0.2 % Triton X-100 / 100mM MgCl2 / 100mM sucrose/ 10mM EGTA Beta on ice] followed by 4 % paraformaldehyde fixation of tissues works well for immunofluorescence of anti-hMre11 . Please see reference: Franchitto, A, Pichierri, P, Blooms syndrome protein is required for correct relocalization of RAD50/Mre11/nbs1 complex after replication fork arrest. J. of Cell Biology, DOI: 10 (2002)Immunohistochemistry - FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Buffer Whole antisera
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (ABIN151076) Western Blot analysis of Mre11 on 50 ug of HeLa and MEF lysates, using ABIN151076
Immunohistochemistry (IHC) image for anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (ABIN151076) MRE11 staining in the human epidermis detected with ABIN151076.
Simple Western (SimWes) image for anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (ABIN151076) Simple Western: Mre11 Antibody [ABIN151076] - Simple Western lane view shows a specif...
Western Blotting (WB) image for anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (ABIN151076) Western Blot: Mre11 Antibody [ABIN151076] - TIG-1 human primary fibroblasts, whole ce...
Immunofluorescence (IF) image for anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (ABIN151076) Immunocytochemistry/Immunofluorescence: Mre11 Antibody [ABIN151076] - IF on HeLa. Ima...
Immunofluorescence (IF) image for anti-MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae) (MRE11A) antibody (ABIN151076) Immunocytochemistry/Immunofluorescence: Mre11 Antibody [ABIN151076] - HeLa cells were...
Product cited in: Wimmer, Berscheminski, Blanchette, Groitl, Branton, Hay, Dobner, Schreiner: "PML isoforms IV and V contribute to adenovirus-mediated oncogenic transformation by functionally inhibiting the tumor-suppressor p53." in: Oncogene, Vol. 35, Issue 1, pp. 69-82, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Obermeier, Sachsenweger, Friedl, Pospiech, Winqvist, Wiesmüller: "Heterozygous PALB2 c.1592delT mutation channels DNA double-strand break repair into error-prone pathways in breast cancer patients." in: Oncogene, Vol. 35, Issue 29, pp. 3796-806, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Mayer, Baran, Sakakibara, Brzakova, Ferencova, Motlik, Kitajima, Schultz, Solc: "DNA damage response during mouse oocyte maturation." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 4, pp. 546-58, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Choi, Meghani, Brault, Leclerc, He, Day, Elias, Drapkin, Weinstock, Dao, Shih, Matulonis, Levine, Konstantinopoulos, Chowdhury: "Platinum and PARP Inhibitor Resistance Due to Overexpression of MicroRNA-622 in BRCA1-Mutant Ovarian Cancer." in: Cell reports, Vol. 14, Issue 3, pp. 429-39, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Hampp, Kiessling, Buechle, Mansilla, Thomale, Rall, Ahn, Pospiech, Gottifredi, Wiesmüller: "DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, Issue 30, pp. E4311-9, 2016 (PubMed). Further details: Western Blotting

Cirilli, Bereshchenko, Ermakova, Nerlov: "Insights into specificity, redundancy and new cellular functions of C/EBPa and C/EBPb transcription factors through interactome network analysis." in: Biochimica et biophysica acta, Vol. 1861, Issue 2, pp. 467-476, 2016 (PubMed).

Speiseder, Hofmann-Sieber, Rodríguez, Schellenberg, Akyüz, Dierlamm, Spruss, Lange, Dobner: "Efficient Transformation of Primary Human Mesenchymal Stromal Cells by Adenovirus Early Region 1 Oncogenes." in: Journal of virology, Vol. 91, Issue 1, 2016 (PubMed).

Xu, Song, Li, Tu, Liu, Liu, Wang, Wang, Cui, Liu, Shang, de Rooij, Gao, Li: "H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation." in: Nucleic acids research, Vol. 44, Issue 20, pp. 9681-9697, 2016 (PubMed).

Badie, Carlos, Folio, Okamoto, Bouwman, Jonkers, Tarsounas: "BRCA1 and CtIP promote alternative non-homologous end-joining at uncapped telomeres." in: The EMBO journal, Vol. 34, Issue 3, pp. 410-24, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Parameswaran, Chiang, Lu, Coates, Deng, Baer, Lin, Li, Paull, Hu: "Damage-induced BRCA1 phosphorylation by Chk2 contributes to the timing of end resection." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 3, pp. 437-48, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence

Kondratova, Watanabe, Marotta, Cannon, Segall, Serre, Tanaka: "Replication fork integrity and intra-S phase checkpoint suppress gene amplification." in: Nucleic acids research, Vol. 43, Issue 5, pp. 2678-90, 2015 (PubMed). (Sample species: Hamster). Further details: Western Blotting

Patel, Puck, Srinivasan, Brown, Sunderam, Kundu, Brenner, Gatti, Church: "Nijmegen breakage syndrome detected by newborn screening for T cell receptor excision circles (TRECs)." in: Journal of clinical immunology, Vol. 35, Issue 2, pp. 227-33, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Thangavel, Berti, Levikova, Pinto, Gomathinayagam, Vujanovic, Zellweger, Moore, Lee, Hendrickson, Cejka, Stewart, Lopes, Vindigni: "DNA2 drives processing and restart of reversed replication forks in human cells." in: The Journal of cell biology, Vol. 208, Issue 5, pp. 545-62, 2015 (PubMed). (Sample species: Human).

Kobayashi, Saito, Okui, Miwa, Komatsu: "Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses." in: Mutation research. Genetic toxicology and environmental mutagenesis, Vol. 782, pp. 42-50, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Xu, Wu, Her: "hMSH5 Facilitates the Repair of Camptothecin-induced Double-strand Breaks through an Interaction with FANCJ." in: The Journal of biological chemistry, Vol. 290, Issue 30, pp. 18545-58, 2015 (PubMed). (Sample species: Human).

Muraki, Han, Miller, Murnane: "Processing by MRE11 is involved in the sensitivity of subtelomeric regions to DNA double-strand breaks." in: Nucleic acids research, Vol. 43, Issue 16, pp. 7911-30, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Lee, Im, Shibata, Park, Handa, Kowalczykowski, Dutta: "MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex." in: Nature communications, Vol. 6, pp. 7744, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Kijas, Lim, Bolderson, Cerosaletti, Gatei, Jakob, Tobias, Taucher-Scholz, Gueven, Oakley, Concannon, Wolvetang, Khanna, Wiesmüller, Lavin: "ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1." in: Nucleic acids research, Vol. 43, Issue 17, pp. 8352-67, 2015 (PubMed). (Sample species: Human). Further details: Immunoprecipitation

Iannascoli, Palermo, Murfuni, Franchitto, Pichierri: "The WRN exonuclease domain protects nascent strands from pathological MRE11/EXO1-dependent degradation." in: Nucleic acids research, Vol. 43, Issue 20, pp. 9788-803, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Liu, Chandramouly, Rass, Guan, Wang, Hobbs, Rajendran, Xie, Shah, Davis, Scully, Lunardi, Pandolfi: "LRF maintains genome integrity by regulating the non-homologous end joining pathway of DNA repair." in: Nature communications, Vol. 6, pp. 8325, 2015 (PubMed). Further details: Immunohistochemistry