NLRP2 antibody (NLR Family, Pyrin Domain Containing 2)

Details for Product anti-NLRP2 Antibody No. ABIN151077, Supplier: Log in to see
Antigen
  • NLRP2
  • NALP2
  • nbs
  • nbs1
  • Nbs1
  • CLR19.9
  • NBS1
  • PAN1
  • PYPAF2
  • E330007A02Rik
  • Nalp2
  • NLR family, pyrin domain containing 2
  • nibrin
  • NLRP2
  • LOC100344125
  • nbn
  • Nbn
  • Nlrp2
Alternatives
anti-Primate NLRP2 antibody for Chromatin Immunoprecipitation
Reactivity
Hamster, Human, Mammalian, Mouse (Murine), Primate
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73
29
4
3
3
2
2
1
1
Host
Rabbit
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31
26
3
Clonality
Polyclonal
Conjugate
This NLRP2 antibody is un-conjugated
7
7
5
5
4
4
4
4
4
3
3
3
3
3
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
154
57
56
38
33
29
25
10
4
3
2
2
1
1
1
Options
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Immunogen Partial length human NBS1 protein [Swiss-Prot# O60934].
Purification Unpurified
Alternative Name Nbs1 (NLRP2 Antibody Abstract)
Background Gene Symbol: NBN
Molecular Weight Theoretical MW: 95 kDa
Gene ID 4683
UniProt O60934
Pathways Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Application Notes Western Blot 1:1000, Chromatin Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 3 μLThis NBS1 antibody is useful for Western blot, Immunoprecipitation and Immunocytochemistry/Immunofluorescence. Use in Chromatin Immunoprecipitation was reported in scientific literature (PMID: 23681918). By WB, this NBS1 antibody recognizes a band at 95 kDa, representing NBS1. By ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. IP tests have been done with 3-4 X 106 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for NBS1 Antibody Western Blot Procedure
1. Run ~50ug of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/3 % BSA for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with NB100-143 (anti-hp95/nibrin), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with goat anti-rabbit IgG-HRP, diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. Note: HeLa whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Buffer Whole antisera
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-NLRP2 antibody (NLR Family, Pyrin Domain Containing 2) (ABIN151077) Detection of human NBS1 using ABIN151077.
Western Blotting (WB) image for anti-NLRP2 antibody (NLR Family, Pyrin Domain Containing 2) (ABIN151077) Western Blot: Nbs1 Antibody [ABIN151077] - TIG-1 human primary fibroblasts, whole cel...
Western Blotting (WB) image for anti-NLRP2 antibody (NLR Family, Pyrin Domain Containing 2) (ABIN151077) Western Blot: Nbs1 Antibody [ABIN151077] - Detection of NBS1 in Human Bone Osteosarco...
Immunofluorescence (IF) image for anti-NLRP2 antibody (NLR Family, Pyrin Domain Containing 2) (ABIN151077) Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [ABIN151077] - Staining of Hela...
Immunofluorescence (IF) image for anti-NLRP2 antibody (NLR Family, Pyrin Domain Containing 2) (ABIN151077) Immunocytochemistry/Immunofluorescence: Nbs1 Antibody [ABIN151077] - Hela cells. Imag...
Product cited in: Smith, Gole, Willis, Soria, Starnes, Krumpelbeck, Jegga, Ali, Guo, Meetei, Andreassen, Kappes, Vinnedge, Daniel, Scully, Wiesmüller, Wells: "DEK is required for homologous recombination repair of DNA breaks." in: Scientific reports, Vol. 7, pp. 44662, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Mariggiò, Koch, Zhang, Weidner-Glunde, Rückert, Kati, Santag, Schulz et al.: "Kaposi Sarcoma Herpesvirus (KSHV) Latency-Associated Nuclear Antigen (LANA) recruits components of the MRN (Mre11-Rad50-NBS1) repair complex to modulate an innate immune signaling pathway and viral ..." in: PLoS pathogens, Vol. 13, Issue 4, pp. e1006335, 2017 (PubMed).

Ward, McHugh, Durant: "Small molecule inhibitors uncover synthetic genetic interactions of human flap endonuclease 1 (FEN1) with DNA damage response genes." in: PLoS ONE, Vol. 12, Issue 6, pp. e0179278, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Francia, Cabrini, Matti, Oldani, dAdda di Fagagna: "DICER, DROSHA and DNA damage response RNAs are necessary for the secondary recruitment of DNA damage response factors." in: Journal of cell science, Vol. 129, Issue 7, pp. 1468-76, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Mlody, Adjaye: "Generation of iPSC lines from a Nijmegen Breakage Syndrome patient." in: Stem cell research, Vol. 15, Issue 3, pp. 629-32, 2016 (PubMed).

Lou, Kim, Meyerson, Pancholi, Mohni, Enard, Petrov, Weller, Weitzman, Sawyer: "An Intrinsically Disordered Region of the DNA Repair Protein Nbs1 Is a Species-Specific Barrier to Herpes Simplex Virus 1 in Primates." in: Cell host & microbe, Vol. 20, Issue 2, pp. 178-88, 2016 (PubMed). Further details: Western Blotting

Xu, Song, Li, Tu, Liu, Liu, Wang, Wang, Cui, Liu, Shang, de Rooij, Gao, Li: "H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation." in: Nucleic acids research, Vol. 44, Issue 20, pp. 9681-9697, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Campos-León, Wijendra, Siddiqa, Pentland, Feeney, Knapman, Davies, Androphy, Parish: "Association of Human Papillomavirus 16 E2 with Rad50-Interacting Protein 1 Enhances Viral DNA Replication." in: Journal of virology, Vol. 91, Issue 5, 2016 (PubMed). Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).

Xie, Park, You, Li, Owonikoko, Wang, Doetsch, Deng: "Bcl2 inhibits recruitment of Mre11 complex to DNA double-strand breaks in response to high-linear energy transfer radiation." in: Nucleic acids research, Vol. 43, Issue 2, pp. 960-72, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation

Nakahara, Tanaka, Ohno, Egawa, Yugawa, Kiyono: "Activation of NF-κB by human papillomavirus 16 E1 limits E1-dependent viral replication through degradation of E1." in: Journal of virology, Vol. 89, Issue 9, pp. 5040-59, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Vermezovic, Adamowicz, Santarpia, Rustighi, Forcato, Lucano, Massimiliano, Costanzo, Bicciato, Del Sal, dAdda di Fagagna: "Notch is a direct negative regulator of the DNA-damage response." in: Nature structural & molecular biology, Vol. 22, Issue 5, pp. 417-24, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Goldstein, Kastan: "Repair versus Checkpoint Functions of BRCA1 Are Differentially Regulated by Site of Chromatin Binding." in: Cancer research, Vol. 75, Issue 13, pp. 2699-707, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation,Chromatin Immunoprecipitation

Rein, Yanez, Terré, Palenzuela, Aivio, Wei, Edelmann, Stark, Stracker: "EXO1 is critical for embryogenesis and the DNA damage response in mice with a hypomorphic Nbs1 allele." in: Nucleic acids research, Vol. 43, Issue 15, pp. 7371-87, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Xing, Wang, Palmai-Pallag, Shen, Helleday, Hickson, Ying: "Acute MUS81 depletion leads to replication fork slowing and a constitutive DNA damage response." in: Oncotarget, Vol. 6, Issue 35, pp. 37638-46, 2015 (PubMed).

Patel, Puck, Srinivasan, Brown, Sunderam, Kundu, Brenner, Gatti, Church: "Nijmegen breakage syndrome detected by newborn screening for T cell receptor excision circles (TRECs)." in: Journal of clinical immunology, Vol. 35, Issue 2, pp. 227-33, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Kobayashi, Saito, Okui, Miwa, Komatsu: "Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses." in: Mutation research. Genetic toxicology and environmental mutagenesis, Vol. 782, pp. 42-50, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Chen, Ebelt, Stracker, Xhemalce, Van Den Berg, Miller: "ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion." in: eLife, Vol. 4, 2015 (PubMed). Further details: Western Blotting

Lee, Im, Shibata, Park, Handa, Kowalczykowski, Dutta: "MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex." in: Nature communications, Vol. 6, pp. 7744, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Kijas, Lim, Bolderson, Cerosaletti, Gatei, Jakob, Tobias, Taucher-Scholz, Gueven, Oakley, Concannon, Wolvetang, Khanna, Wiesmüller, Lavin: "ATM-dependent phosphorylation of MRE11 controls extent of resection during homology directed repair by signalling through Exonuclease 1." in: Nucleic acids research, Vol. 43, Issue 17, pp. 8352-67, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Boulware, Christensen, Thames, Coghlan, Vasquez, Finch: "Triplex-forming oligonucleotides targeting c-MYC potentiate the anti-tumor activity of gemcitabine in a mouse model of human cancer." in: Molecular carcinogenesis, Vol. 53, Issue 9, pp. 744-52, 2014 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation