Nibrin (NBN) antibody

Antigen: Nibrin (NBN)

Synonyms: ATV, NBS, P95, NBS1, AT-V1, AT-V2, FLJ10155, MGC87362, Nbs1, NBN, DKFZp469A0911

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN151077

Additional Information

Alternative name: Nibrin

Gene ID: 4683, 27354

UniProt: O60934

Immunogen: Human NBS1 protein (betwqeen residues 1-705). [Swiss-Prot# O60934].

Cross-Reactivity: Human

Format: Serum

Description: The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50double-strand break complex. This protein complex has been implicated in Nijmegenbreakage syndrome, an autosomal recessive disorder marked by increased cancerincidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealinga direct molecular link between double-strand break repair and cell cycle checkpointfunctions. Alternate Names: anti-AT V1 antibody, anti-AT V2 antibody, anti-ATV antibody, anti-NBS antibody,anti-Nibrin antibody, anti-Nijmegen breakage syndrome antibody, anti-p95 antibody.

Specificity: This antibody is specific for human NBS1. Species Reactivity: ABIN151077 recognizes human NBS1 protein. This antibody does not work on mouseprotein in Western blot analysis. Other species have not been tested. Localization: Nuclear.

Application Details

Application Notes: Positive Controls: TK6 and HeLa whole cell extract (NB 800-PC1)

Purification: Serum

Buffer: Whole antisera. Preservative: 0.02% sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Detection of human NBS1 using ABIN151077.

Publications

Gatei, Young, Cerosaletti et al.: "ATM-dependent phosphorylation of nibrin in response to radiation exposure." in: Nature genetics, Vol. 25, Issue 1, pp. 115-9, 2000 (PubMed).

Wu, Petrini, Heine et al.: "Independence of R/M/N focus formation and the presence of intact BRCA1." in: Science (New York, N.Y.), Vol. 289, Issue 5476, pp. 11, 2000 (PubMed).

Paull, Rogakou, Yamazaki et al.: "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage." in: Current biology : CB, Vol. 10, Issue 15, pp. 886-95, 2000 (PubMed).

Harfst, Cooper, Neubauer et al.: "Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome." in: Molecular immunology, Vol. 37, Issue 15, pp. 915-29, 2001 (PubMed).

Buscemi, Savio, Zannini et al.: "Chk2 activation dependence on Nbs1 after DNA damage." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5214-22, 2001 (PubMed).

Huang, Dynan: "Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction." in: Nucleic acids research, Vol. 30, Issue 3, pp. 667-74, 2002 (PubMed).

Stracker, Carson, Weitzman: "Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex." in: Nature, Vol. 418, Issue 6895, pp. 348-52, 2002 (PubMed).

Goldberg, Stucki, Falck et al.: "MDC1 is required for the intra-S-phase DNA damage checkpoint." in: Nature, Vol. 421, Issue 6926, pp. 952-6, 2003 (PubMed).

Cerosaletti, Concannon: "Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21944-51, 2003 (PubMed).

Siwicki, Degerman, Chrzanowska et al.: "Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients." in: Experimental cell research, Vol. 287, Issue 1, pp. 178-89, 2003 (PubMed).