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Nibrin antibody (NBN)

Details for Product anti-NBN Antibody No. ABIN151077, Supplier: Login to see New
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(263), (60), (50), (4), (1)
(191), (78)
This Nibrin antibody is un-conjugated
(6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(224), (71), (58), (51), (42), (29), (24), (22), (18), (5), (3), (2), (1)
Pubmed 10 references available
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Quantity 50 μL
Shipping to United States ( )
Immunogen Partial length human NBS1 protein
Cross-Reactivity (Details) Mammal reactivity reported in scientific literature (PMID: 24064949)
Purification Whole antisera
Alternative Name Nibrin (NBN Antibody Abstract)
Background The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50double-strand break complex. This protein complex has been implicated in Nijmegenbreakage syndrome, an autosomal recessive disorder marked by increased cancerincidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealinga direct molecular link between double-strand break repair and cell cycle checkpointfunctions. Alternate Names: anti-AT V1 antibody, anti-AT V2 antibody, anti-ATV antibody, anti-NBS antibody,anti-Nibrin antibody, anti-Nijmegen breakage syndrome antibody, anti-p95 antibody.
Gene Symbol: NBN
Gene ID 4683, 27354
UniProt O60934
Pathways DNA Damage Repair
Application Notes This NBS1 antibody is useful for Western blot, Immunoprecipitation and Immunocytochemistry/Immunofluorescence. Use in Chromatin Immunoprecipitation was reported in scientific literature (PMID: 23681918). By WB, this NBS1 antibody recognizes a band at 95 kDa, representing NBS1. By ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. IP tests have been done with 3-4 X 106 cells.
Recommended dilutions: Chromatin Immunoprecipitation, Flow Cytometry, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry, Immunoprecipitation 3 µL, Western Blot 1:1000
Protocol Protocol specific for NBS1 Antibody Western Blot Procedure
1. Run ~50ug of protein on a 4-20 % Tris-glycine mini-gel at 125V for 90 minutes.
. Equilibrate gel, nitrocellulose membrane, Whatman paper, and blotting pads in transfer buffer for 15 minutes.
. Transfer protein to the membrane at 25V for 90 minutes.
. Allow membrane to air-dry.
. Block membrane with 1XPBS/3 % BSA for 1 hour at room temperature (~23-27 degrees C).
. Wash membrane twice, for 5 minutes each, with 1XPBS/0.05 % Tween-20 (PBST).
. Incubate membrane with NB100-143 (anti-hp95/nibrin), diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Incubate membrane with goat anti-rabbit IgG-HRP, diluted in 1XPBS/1 % BSA, for 1 hour at room temperature.
. Wash membrane once for 15 minutes, then four times for 5 minutes each, with PBST.
. Detect cross-reacting proteins using Renaissance Chemiluminescence Reagent Plus kit from NEN Life Sciences. Note: HeLa whole cell extracts were used as a positive control for this antibody.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-Nibrin antibody (NBN) (ABIN151077) Detection of human NBS1 using ABIN151077.
Product cited in: Siwicki, Degerman, Chrzanowska et al.: "Telomere maintenance and cell cycle regulation in spontaneously immortalized T-cell lines from Nijmegen breakage syndrome patients." in: Experimental cell research, Vol. 287, Issue 1, pp. 178-89, 2003 (PubMed).

Cerosaletti, Concannon: "Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21944-51, 2003 (PubMed).

Goldberg, Stucki, Falck et al.: "MDC1 is required for the intra-S-phase DNA damage checkpoint." in: Nature, Vol. 421, Issue 6926, pp. 952-6, 2003 (PubMed).

Stracker, Carson, Weitzman: "Adenovirus oncoproteins inactivate the Mre11-Rad50-NBS1 DNA repair complex." in: Nature, Vol. 418, Issue 6895, pp. 348-52, 2002 (PubMed).

Huang, Dynan: "Reconstitution of the mammalian DNA double-strand break end-joining reaction reveals a requirement for an Mre11/Rad50/NBS1-containing fraction." in: Nucleic acids research, Vol. 30, Issue 3, pp. 667-74, 2002 (PubMed).

Paull, Rogakou, Yamazaki et al.: "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage." in: Current biology : CB, Vol. 10, Issue 15, pp. 886-95, 2000 (PubMed).

Wu, Petrini, Heine et al.: "Independence of R/M/N focus formation and the presence of intact BRCA1." in: Science (New York, N.Y.), Vol. 289, Issue 5476, pp. 11, 2000 (PubMed).

Gatei, Young, Cerosaletti et al.: "ATM-dependent phosphorylation of nibrin in response to radiation exposure." in: Nature genetics, Vol. 25, Issue 1, pp. 115-9, 2000 (PubMed).

Background publications Buscemi, Savio, Zannini et al.: "Chk2 activation dependence on Nbs1 after DNA damage." in: Molecular and cellular biology, Vol. 21, Issue 15, pp. 5214-22, 2001 (PubMed).

Harfst, Cooper, Neubauer et al.: "Normal V(D)J recombination in cells from patients with Nijmegen breakage syndrome." in: Molecular immunology, Vol. 37, Issue 15, pp. 915-29, 2001 (PubMed).

Catalog No. ABIN151077

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