anti-RAD51 antibody (DNA Repair Protein Homolog 1) (AA 1-338)

Details for Product anti-RAD51 Antibody No. ABIN151080
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AA 1-338
(23), (19), (19), (17), (12), (12), (6), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
(198), (100), (62), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(154), (50), (5)
Clonality (Clone)
Monoclonal ()
This RAD51 antibody is un-conjugated
(7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (1), (1)
Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(147), (62), (50), (44), (40), (32), (20), (8), (7), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 11 references available
Quantity 0.1 mg
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Immunogen Full length (amino acids 1-338) Rad51 expressed in E. coli.
Clone 14B4
Isotype IgG2b
Cross-Reactivity (Details) Reactivity with C.elegans reported in scientific literature (PMID: 23942865). Based upon sequence homology, predicted to react with: Monkey/Primates (100%), Bovine (99%), Dog (99%), Chinese hamster (99%), Rabbit (99%), Porcine/Pig (99%),Xenopus (95%). RAD51 is highly conserved in eukaryotes,this antibody may have wide range of species reactivity.
Purification Protein G purified
Alternative Name RAD51 (RAD51 Antibody Abstract)
Background Rad51, a 37kDa protein, is the human homolog of E. coli RecA protein and a member ofthe RAD52 epistasis group in S. cerevisiae. In recent studies it has been reported thatBRCA1 interacts with Rad51 and disease-causing mutations have been found in theBRCA1 region necessary for BRCA1/Rad51 interaction, implying that this interaction isimportant for tumor suppression. BRCA2 and Rad51 have also been shown to interactby direct binding of the BRC repeats located within exon 11 of BRCA2. When theserepeats are deleted, the interaction is lost and cells become hypersensitive to the DNAdamage caused by methyl methanesulfonate (MMS). Alternate Names: anti-DNA repair protein RAD51 homolog 1 antibody, anti-E coli RecA homolog antibody,anti-HGNC:9817 antibody, anti-Homolog of E coli RecA antibody, anti-homolog of Scerevisiae RAD51 antibody, anti-HRAD51 antibody, anti-HsRad51 antibody,anti-HsT16930 antibody, anti-RAD51 S cerevisiae homolog antibody, anti-RAD51Aantibody, anti-RECA antibody, anti-RecA like protein antibody, anti-recombinationprotein A antibody.
Gene Symbol: RAD51
Gene ID 5888
Application Notes Use in Chromatin immunoprecipitation reported in scientific literature (PMID 24023853)
Recommended dilutions: Chromatin Immunoprecipitation 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:1000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Immunohistochemistry-Paraffin 1:2000, Immunoprecipitation 1:10-1:500, Western Blot 1:500-1:2000
Restrictions For Research Use only
Buffer PBS pH 7.4
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-RAD51 antibody (DNA Repair Protein Homolog 1) (AA 1-338) (ABIN151080) anti-DNA Repair Protein Homolog 1 (RAD51) (AA 1-338) antibody
General Stewart, Stankovic, Byrd et al.: "RIDDLE immunodeficiency syndrome is linked to defects in 53BP1-mediated DNA damage signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 43, pp. 16910-5, 2007 (PubMed).

Shim, Schmutte, Tombline et al.: "hXRCC2 enhances ADP/ATP processing and strand exchange by hRAD51." in: The Journal of biological chemistry, Vol. 279, Issue 29, pp. 30385-94, 2004 (PubMed).

Warren, Lord, Masabanda et al.: "Phenotypic effects of heterozygosity for a BRCA2 mutation." in: Human molecular genetics, Vol. 12, Issue 20, pp. 2645-56, 2003 (PubMed).

Kao, McKenna, Guenther et al.: "Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response." in: The Journal of cell biology, Vol. 160, Issue 7, pp. 1017-27, 2003 (PubMed).

Tarsounas, Davies, West: "BRCA2-dependent and independent formation of RAD51 nuclear foci." in: Oncogene, Vol. 22, Issue 8, pp. 1115-23, 2003 (PubMed).

French, Masson, Griffin et al.: "Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19322-30, 2002 (PubMed).

Masson, Tarsounas, Stasiak et al.: "Identification and purification of two distinct complexes containing the five RAD51 paralogs." in: Genes & development, Vol. 15, Issue 24, pp. 3296-307, 2001 (PubMed).

Masson, Davies, Hajibagheri et al.: "The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51." in: The EMBO journal, Vol. 18, Issue 22, pp. 6552-60, 2000 (PubMed).

Chen, Chen, Chen et al.: "The BRC repeats in BRCA2 are critical for RAD51 binding and resistance to methyl methanesulfonate treatment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 9, pp. 5287-92, 1998 (PubMed).

Scully, Chen, Plug et al.: "Association of BRCA1 with Rad51 in mitotic and meiotic cells." in: Cell, Vol. 88, Issue 2, pp. 265-75, 1997 (PubMed).

Shinohara, Ogawa, Matsuda et al.: "Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA." in: Nature genetics, Vol. 4, Issue 3, pp. 239-43, 1993 (PubMed).

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Catalog No. ABIN151080
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