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RAD51 antibody (DNA Repair Protein Homolog 1)

Details for Product anti-RAD51 Antibody No. ABIN151080, Supplier: Log in to see
Antigen
  • 4921524O04Rik
  • 8030409M04Rik
  • ATRAD51
  • AV304093
  • brcc5
  • BRCC5
  • CRAD51
  • F22D1.20
  • F22D1_20
  • HRAD51
  • HsRad51
  • HsT16930
  • MRMV2
  • rad51
  • RAD51
  • rad51a
  • RAD51A
  • Rad51a
  • RAS associated with diabetes protein 51
  • RecA
  • Reca
  • reca
  • RECA
  • RGD1563603
  • wu:fb38e12
  • xrad51
  • zgc:77754
Alternatives
anti-Human RAD51 antibody for Immunocytochemistry
Reactivity
Caenorhabditis elegans (C. elegans), Chicken, Human, Mouse (Murine)
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5
2
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2
2
1
1
1
1
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1
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Host
Mouse
119
59
3
Clonality (Clone)
Monoclonal ()
Conjugate
This RAD51 antibody is un-conjugated
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3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
141
53
47
34
34
24
23
15
11
4
2
1
1
1
1
Supplier
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Immunogen Full length (amino acids 1 - 338) Rad51 expressed in E. coli.
Clone 14B4
Isotype IgG2b
Cross-Reactivity (Details) Reactivity with C.elegans reported in scientific literature (PMID: 23942865). Based upon sequence homology, predicted to react with: Monkey/Primates (100%), Bovine (99%), Dog (99%), Chinese hamster (99%), Rabbit (99%), Porcine/Pig (99%),Xenopus (95%). RAD51 is highly conserved in eukaryotes,this antibody may have wide range of species reactivity.
Purification Protein G purified
Alternative Name Rad51 (RAD51 Antibody Abstract)
Background Gene Symbol: RAD51
Molecular Weight Theoretical MW: 37 kDa
Gene ID 5888
UniProt Q06609
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Pathways DNA Damage Repair
Application Notes Western Blot 1:500 - 1:2000, Chromatin Immunoprecipitation 1:10 - 1:500, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:1000, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:2000, Immunohistochemistry-Frozen 1:10 - 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-RAD51 antibody (DNA Repair Protein Homolog 1) (ABIN151080) anti-DNA Repair Protein Homolog 1 (RAD51) antibody
Western Blotting (WB) image for anti-RAD51 antibody (DNA Repair Protein Homolog 1) (ABIN151080) Western Blot: Rad51 Antibody (14B4) [ABIN151080] - Various whole cell extracts (30 ug...
Immunoprecipitation (IP) image for anti-RAD51 antibody (DNA Repair Protein Homolog 1) (ABIN151080) Immunoprecipitation: Rad51 Antibody (14B4) [ABIN151080] - HeLa cell extract prepared ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RAD51 antibody (DNA Repair Protein Homolog 1) (ABIN151080) Immunohistochemistry-Paraffin: Rad51 Antibody (14B4) [ABIN151080] - Detects RAD51 in ...
Immunofluorescence (IF) image for anti-RAD51 antibody (DNA Repair Protein Homolog 1) (ABIN151080) Immunocytochemistry/Immunofluorescence: Rad51 Antibody (14B4) [ABIN151080] - staning ...
Product cited in: Dok, Abbasi Asbagh, Van Limbergen, Sablina, Nuyts: "Nuclear p16INK4a expression predicts enhanced radiation response in head and neck cancers." in: Oncotarget, Vol. 7, Issue 25, pp. 38785-38795, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry

Ackermann, Schell, Pokrzywa, Kevei, Gartner, Schumacher, Hoppe: "E4 ligase-specific ubiquitination hubs coordinate DNA double-strand-break repair and apoptosis." in: Nature structural & molecular biology, Vol. 23, Issue 11, pp. 995-1002, 2016 (PubMed). (Sample species: Caenorhabditis elegans (C. elegans)). Further details: Immunohistochemistry

Li, Chen, Yu: "Chemopreventive Effects of ROS Targeting in a Murine Model of BRCA1-Deficient Breast Cancer." in: Cancer research, Vol. 77, Issue 2, pp. 448-458, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Liu, Yan, Fanning: "Human DNA helicase B functions in cellular homologous recombination and stimulates Rad51-mediated 5'-3' heteroduplex extension in vitro." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116852, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Del Nagro, Choi, Xiao, Rangell, Mohan, Pandita, Zha, Jackson, OBrien: "Chk1 inhibition in p53-deficient cell lines drives rapid chromosome fragmentation followed by caspase-independent cell death." in: Cell cycle (Georgetown, Tex.), Vol. 13, Issue 2, pp. 303-14, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Cornago, Garcia-Alberich, Blasco-Angulo, Vall-Llaura, Nager, Herreros, Comella, Sanchis, Llovera: "Histone deacetylase inhibitors promote glioma cell death by G2 checkpoint abrogation leading to mitotic catastrophe." in: Cell death & disease, Vol. 5, pp. e1435, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Buonanno, Garty, Grad, Gendrel, Hobert, Brenner: "Microbeam irradiation of C. elegans nematode in microfluidic channels." in: Radiation and environmental biophysics, Vol. 52, Issue 4, pp. 531-7, 2013 (PubMed). (Sample species: Caenorhabditis elegans (C. elegans)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Lans, Lindvall, Thijssen, Karambelas, Cupac, Fensgård, Jansen, Hoeijmakers, Nilsen, Vermeulen: "DNA damage leads to progressive replicative decline but extends the life span of long-lived mutant animals." in: Cell death and differentiation, Vol. 20, Issue 12, pp. 1709-18, 2013 (PubMed). (Sample species: Caenorhabditis elegans (C. elegans)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry

Chu, Wu, Xu, Her: "MutS homologue hMSH4: interaction with eIF3f and a role in NHEJ-mediated DSB repair." in: Molecular cancer, Vol. 12, pp. 51, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Wu, Xu, Feng, Tompkins, Her: "MutS homologue hMSH5: recombinational DSB repair and non-synonymous polymorphic variants." in: PLoS ONE, Vol. 8, Issue 9, pp. e73284, 2013 (PubMed). (Sample species: Human). Further details: Chromatin Immunoprecipitation

Moldovan, Dejsuphong, Petalcorin, Hofmann, Takeda, Boulton, DAndrea: "Inhibition of homologous recombination by the PCNA-interacting protein PARI." in: Molecular cell, Vol. 45, Issue 1, pp. 75-86, 2012 (PubMed).

Tompkins, Wu, Her: "MutS homologue hMSH5: role in cisplatin-induced DNA damage response." in: Molecular cancer, Vol. 11, pp. 10, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Lee, Daniels, Garnett, Venkitaraman: "A mitotic function for the high-mobility group protein HMG20b regulated by its interaction with the BRC repeats of the BRCA2 tumor suppressor." in: Oncogene, Vol. 30, Issue 30, pp. 3360-9, 2011 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Harper, Rillo, Jover-Gil, Assaf, Bhalla, Dernburg: "Pairing centers recruit a Polo-like kinase to orchestrate meiotic chromosome dynamics in C. elegans." in: Developmental cell, Vol. 21, Issue 5, pp. 934-47, 2011 (PubMed).

Meerang, Ritz, Paliwal, Garajova, Bosshard, Mailand, Janscak, Hübscher, Meyer, Ramadan: "The ubiquitin-selective segregase VCP/p97 orchestrates the response to DNA double-strand breaks." in: Nature cell biology, Vol. 13, Issue 11, pp. 1376-82, 2011 (PubMed).

Bowen, Gelmann: "NKX3.1 activates cellular response to DNA damage." in: Cancer research, Vol. 70, Issue 8, pp. 3089-97, 2010 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Graeser, McCarthy, Lord, Savage, Hills, Salter, Orr, Parton, Smith, Reis-Filho, Dowsett, Ashworth, Turner: "A marker of homologous recombination predicts pathologic complete response to neoadjuvant chemotherapy in primary breast cancer." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 16, Issue 24, pp. 6159-68, 2010 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Ammazzalorso, Pirzio, Bignami, Franchitto, Pichierri: "ATR and ATM differently regulate WRN to prevent DSBs at stalled replication forks and promote replication fork recovery." in: The EMBO journal, Vol. 29, Issue 18, pp. 3156-69, 2010 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Klammer, Kadhim, Iliakis: "Evidence of an adaptive response targeting DNA nonhomologous end joining and its transmission to bystander cells." in: Cancer research, Vol. 70, Issue 21, pp. 8498-506, 2010 (PubMed). Further details: Immunocytochemistry,Immunofluorescence

Moldovan, Madhavan, Mirchandani, McCaffrey, Vinciguerra, DAndrea: "DNA polymerase POLN participates in cross-link repair and homologous recombination." in: Molecular and cellular biology, Vol. 30, Issue 4, pp. 1088-96, 2010 (PubMed).