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RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 628-787) antibody

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AA 628-787
(20), (15), (9), (6), (6), (5), (4), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(79), (37), (11), (3), (2)
(85), (15), (4)
(5), (4), (2), (2), (2), (2)
Western Blotting (WB), Immunoprecipitation (IP)
(90), (38), (35), (17), (10), (7), (7), (6), (1), (1), (1)
Pubmed 2 references available
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Quantity 0.1 mL
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Immunogen Human RAD50 zinc hook region (amino acids 628-787) expressed in E. coli.
Purification Whole antisera
Alternative Name RAD50 (RAD50 Antibody Abstract)
Background Rad50, a 153kDa protein, is associated with Mre11 and Nbs1 (Nibrin or p95) to form amultiprotein complex involved in the double strand break repair process. Rad50 is amember of the family of structural maintenance of chromosome (SMC) proteins. Members of this family share similar structures consisting of globular N- and C-terminiseparated by a coiled-coil domain. At the base of the coiled-coil region is a zinc bindingmotif, the zinc hook. The zinc hook mediates Rad50 dimerization through the linkage oftwo Rad50 coiled-coil tails. Alternate Names: anti-hRad50 antibody, anti-hsRAD50 antibody, anti-Mrell antibody, anti-RAD50 2antibody, anti-RAD50 homolog (S cerevisiae) antibody, anti-RAD50 PEN antibody,anti-Rad50l antibody, anti-Truncated RAD50 protein antibody.
Gene Symbol: RAD50
Gene ID 10111
UniProt Q92878
Pathways DNA Damage Repair
Application Notes This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at approximately 153 kDa.
Recommended dilutions: immunoprecipitation 1-5µL, Western Blot 1:1000-1:10000
Protocol Western blot protocol specific for RAD50 Antibody Western blot
1. Gel/Transfer set up according to manufacturer's instructions (NuPAGE, Invitrogen).
. Block: 1X TBST +5 % milk (TBST: 100mM Tris pH 7.5, 150mM NaCl, 0.1 % Tween) for 1 hour at room temperature.
. Wash 3 times (5 minutes) in 1X TBST.
. Primary Antibody: Rad50, hook serum diluted 1:5,000 in 1X TBST and incubated for 1 hour at room temperature.
. Wash 3 times (10 minutes) in 1X TBST.
. Secondary Antibody: Anti-rabbit IgG diluted in 1X TBST and incubated for 1 hour at room temperature.
. Wash 3 times (5 minutes) in 1X TBST.
. Expose: Chemiluminescent HRP for 15-60 seconds. NOTE: This is the protocol used to obtain the image on the datasheet.
Restrictions For Research Use only
Format Liquid
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
Supplier Images
Western Blotting (WB) image for anti-RAD50 Homolog (S. Cerevisiae) (RAD50) (AA 628-787) antibody (ABIN151081) Detection of Rad50 using ABIN151081.Lane 1: 25 ng purified hMre11-hRad50 complexLane ...
Product cited in: Cahill, Carney: "Dimerization of the Rad50 protein is independent of the conserved hook domain." in: Mutagenesis, Vol. 22, Issue 4, pp. 269-74, 2007 (PubMed).

Background publications Carney, Maser, Olivares et al.: "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response." in: Cell, Vol. 93, Issue 3, pp. 477-86, 1998 (PubMed).

Catalog No. ABIN151081

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