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RAD50 Homolog (S. Cerevisiae) (RAD50) antibody

Antigen

RAD50 Homolog (S. Cerevisiae) (RAD50)
Synonyms hRad50, RAD50-2, Mrell, Rad50l, Dmrad50, RAD50, DmelCG6339, CG6339, ATRAD50, DNA REPAIR-RECOMBINATION PROTEIN, F22D22.28, F22D22_28, rad50
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunoprecipitation (IP), Western Blotting (WB)
2 references available
Catalog no. ABIN151081
Quantity 0.1 ml  (Variants)
Price Product not available in this region.
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anti-RAD50 Homolog (S. Cerevisiae) (RAD50) antibody

Additional Information
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Alternative name RAD50
Gene ID 10111
UniProt Q92878
Immunogen Human Rad50 zinc hook region (amino acids 628-787) expressed in E. coli.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Format Serum
Description Rad50, a 153kDa protein, is associated with Mre11 and Nbs1 (Nibrin or p95) to form amultiprotein complex involved in the double strand break repair process. Rad50 is amember of the family of structural maintenance of chromosome (SMC) proteins. Members of this family share similar structures consisting of globular N- and C-terminiseparated by a coiled-coil domain. At the base of the coiled-coil region is a zinc bindingmotif, the zinc hook. The zinc hook mediates Rad50 dimerization through the linkage oftwo Rad50 coiled-coil tails. Alternate Names: anti-hRad50 antibody, anti-hsRAD50 antibody, anti-Mrell antibody, anti-RAD50 2antibody, anti-RAD50 homolog (S cerevisiae) antibody, anti-RAD50 PEN antibody,anti-Rad50l antibody, anti-Truncated RAD50 protein antibody.
Specificity This antibody is specific for human Rad50. Species Reactivity: Reacts with human Rad50. Other species have not been tested. Localization: Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within thenucleus of damaged cells.

Application Details
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Application Notes Suggested working dilutions: immunoprecipitation 1-5ul ,Western Blot 1:1000-1:10000
Purification Serum
Buffer 0.1 ml of whole rabbit antisera. It does not contain any preservative.
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.
Restrictions For Research Use only

Publications
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Carney, Maser, Olivares et al.: "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response." in: Cell, Vol. 93, Issue 3, pp. 477-86, 1998 (PubMed).

Cahill, Carney: "Dimerization of the Rad50 protein is independent of the conserved hook domain." in: Mutagenesis, Vol. 22, Issue 4, pp. 269-74, 2007 (PubMed).

Alternatives
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Alternatives for antigen "RAD50 Homolog (S. Cerevisiae) (RAD50)", type "Antibodies"
Hosts Rabbit (37), Mouse (5), Sheep (2)
Reactivities Human (43), Mouse (Murine) (21), Rat (Rattus) (20), Cow (Bovine) (18), Horse (Equine) (18), Rabbit (18), Sheep (Ovine) (18)
Applications Western Blotting (WB) (29), Immunofluorescence (IF) (16), Immunoprecipitation (IP) (15), ELISA (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (IHC) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Biotin (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes AA 817-872 (18), AA 1-425 (4), N-Term (3), AA 480-724 (2), AA 50-100 (2), AA 628-787 (1), C-Term (1), Internal Region (1)