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RAD50 antibody (RAD50 Homolog (S. Cerevisiae))

Details for Product anti-RAD50 Antibody No. ABIN151081, Supplier: Log in to see
Antigen
  • 6339
  • ATRAD50
  • CG6339
  • Dmel\\CG6339
  • Dmrad50
  • DNA REPAIR-RECOMBINATION PROTEIN
  • F22D22.28
  • F22D22_28
  • hRad50
  • Mrell
  • NBSLD
  • Rad50
  • RAD50
  • Rad50l
  • RAD502
  • SPAP4C9.01c
Alternatives
anti-Human RAD50 antibody for Immunocytochemistry
Reactivity
Human
99
57
29
14
3
2
2
Host
Rabbit
89
30
5
Clonality
Polyclonal
Conjugate
This RAD50 antibody is un-conjugated
6
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
109
50
38
25
21
19
10
4
2
1
1
1
1
Supplier
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Available images

Immunogen Human RAD50 zinc hook region (amino acids 628-787) expressed in E. coli. [UniProt# Q92878]
Purification Unpurified
Alternative Name Rad50 (RAD50 Antibody Abstract)
Background Gene Symbol: RAD50
Molecular Weight Theoretical MW: 153 kDa
Gene ID 10111
UniProt Q92878
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Pathways DNA Damage Repair
Application Notes Western Blot 1:1000-1:10000, Simple Western 1:1000, Immunoprecipitation 1-5 μL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot protocol specific for RAD50 Antibody Western blot
1. Gel/Transfer set up according to manufacturer's instructions (NuPAGE, Invitrogen).
. Block: 1X TBST +5 % milk (TBST: 100mM Tris pH 7.5, 150mM NaCl, 0.1 % Tween) for 1 hour at room temperature.
. Wash 3 times (5 minutes) in 1X TBST.
. Primary Antibody: Rad50, hook serum diluted 1:5,000 in 1X TBST and incubated for 1 hour at room temperature.
. Wash 3 times (10 minutes) in 1X TBST.
. Secondary Antibody: Anti-rabbit IgG diluted in 1X TBST and incubated for 1 hour at room temperature.
. Wash 3 times (5 minutes) in 1X TBST.
. Expose: Chemiluminescent HRP for 15-60 seconds. NOTE: This is the protocol used to obtain the image on the datasheet.
Restrictions For Research Use only
Format Liquid
Buffer Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-RAD50 antibody (RAD50 Homolog (S. Cerevisiae)) (ABIN151081) Detection of Rad50 using ABIN151081.Lane 1: 25 ng purified hMre11-hRad50 complexLane ...
Simple Western (SimWes) image for anti-RAD50 antibody (RAD50 Homolog (S. Cerevisiae)) (ABIN151081) Simple Western: Rad50 Antibody [ABIN151081] - Simple Western lane view shows a specif...
Product cited in: Chang, Huang, Wang, Li, Yan, Zhu, Ye, Wu, Zhuang, Li, Zhang: "Targeting Rad50 sensitizes human nasopharyngeal carcinoma cells to radiotherapy." in: BMC cancer, Vol. 16, pp. 190, 2016 (PubMed).

Kobayashi, Saito, Okui, Miwa, Komatsu: "Increased oxidative stress in AOA3 cells disturbs ATM-dependent DNA damage responses." in: Mutation research. Genetic toxicology and environmental mutagenesis, Vol. 782, pp. 42-50, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Cahill, Carney: "Dimerization of the Rad50 protein is independent of the conserved hook domain." in: Mutagenesis, Vol. 22, Issue 4, pp. 269-74, 2007 (PubMed). (Sample species: Human). Further details: Western Blotting

Background publications Carney, Maser, Olivares, Davis, Le Beau, Yates, Hays, Morgan, Petrini: "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response." in: Cell, Vol. 93, Issue 3, pp. 477-86, 1998 (PubMed).