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FEN1 antibody (Flap Structure-Specific Endonuclease 1)

Details for Product anti-FEN1 Antibody No. ABIN151083, Supplier: Log in to see
Antigen
  • FEN-1
  • FEN1
  • AW538437
  • MF1
  • RAD2
  • cb879
  • fen-1
  • fen1
  • mf1
  • rad2
  • xFEN1a
  • flap structure-specific endonuclease 1
  • flap endonuclease 1-like
  • Flap endonuclease-1
  • flap endonuclease-1
  • flap structure specific endonuclease 1
  • nuclease, Rad2 family
  • FEN1
  • LOC100808044
  • Bm1_49605
  • AT5G26680
  • Fen1
  • fen1
  • fen1-a
Alternatives
anti-Human FEN1 antibody for Immunoprecipitation
Reactivity
Human, Mouse (Murine)
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69
51
6
6
5
5
4
4
2
2
2
1
1
1
1
1
1
Host
Mouse
79
44
Clonality (Clone)
Monoclonal ()
Conjugate
This FEN1 antibody is un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
106
34
26
22
21
19
15
13
6
4
3
1
1
1
Options
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Immunogen Recombinant human FEN-1 protein encoding amino acids 1-380 purified E. coli.
Clone 4E7
Isotype IgG1
Specificity This is specific for FEN-1.
Purification Protein G purified
Alternative Name FEN-1 (FEN1 Antibody Abstract)
Background Gene Symbol: FEN1
Molecular Weight Theoretical MW: 42 kDa
Gene ID 2237
UniProt P39748
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication
Application Notes Western Blot 1:500-1:3000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500WB: Predicted molecular weight: 43 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-FEN1 antibody (Flap Structure-Specific Endonuclease 1) (ABIN151083) Immunocytochemistry/Immunofluorescence: FEN-1 Antibody (4E7) [ABIN151083] - Analysis ...
Western Blotting (WB) image for anti-FEN1 antibody (Flap Structure-Specific Endonuclease 1) (ABIN151083) Western Blot: FEN-1 Antibody (4E7) [ABIN151083] - A: MCF7.
Western Blotting (WB) image for anti-FEN1 antibody (Flap Structure-Specific Endonuclease 1) (ABIN151083) Western Blot: FEN-1 Antibody (4E7) [ABIN151083] - Whole cell extract (30 ug) was sepa...
Product cited in: Jaiswal, Armas, Izumi, Strauss, Narayan: "Adenomatous polyposis coli interacts with flap endonuclease 1 to block its nuclear entry and function." in: Neoplasia (New York, N.Y.), Vol. 14, Issue 6, pp. 495-508, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Ma, Liang, Liu, Axcrona, Kvalheim, Stokke, Nesland, Suo: "Prostate cancer cell lines under hypoxia exhibit greater stem-like properties." in: PLoS ONE, Vol. 6, Issue 12, pp. e29170, 2012 (PubMed).

Tann, Boldogh, Meiss, Qian, Van Houten, Mitra, Szczesny: "Apoptosis induced by persistent single-strand breaks in mitochondrial genome: critical role of EXOG (5'-EXO/endonuclease) in their repair." in: The Journal of biological chemistry, Vol. 286, Issue 37, pp. 31975-83, 2011 (PubMed). (Sample species: Human).

Jaiswal, Narayan: "Assembly of the base excision repair complex on abasic DNA and role of adenomatous polyposis coli on its functional activity." in: Biochemistry, Vol. 50, Issue 11, pp. 1901-9, 2011 (PubMed).

Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." in: DNA repair, Vol. 9, Issue 2, pp. 109-19, 2010 (PubMed). (Sample species: Human).

Guo, Zheng, Xu, Dai, Zhou, Pascua, Chen, Shen: "Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding." in: Nature chemical biology, Vol. 6, Issue 10, pp. 766-73, 2010 (PubMed). (Sample species: Human).

Guo, Zheng, Dai, Zhou, Xu, Shen: "Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity." in: Nucleic acids research, Vol. 37, Issue 10, pp. 3431-41, 2009 (PubMed). (Sample species: Human).

Kong, Mohanty, Stephens, Heale, Gomez-Godinez, Shi, Kim, Yokomori, Berns: "Comparative analysis of different laser systems to study cellular responses to DNA damage in mammalian cells." in: Nucleic acids research, Vol. 37, Issue 9, pp. e68, 2009 (PubMed). (Sample species: Human).

Guo, Chavez, Singh, Finger, Hang, Hegde, Shen: "Comprehensive mapping of the C-terminus of flap endonuclease-1 reveals distinct interaction sites for five proteins that represent different DNA replication and repair pathways." in: Journal of molecular biology, Vol. 377, Issue 3, pp. 679-90, 2008 (PubMed). (Sample species: Human).

Guo, Qian, Liu, Dai, Zhou, Zheng, Shen: "Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair." in: Molecular and cellular biology, Vol. 28, Issue 13, pp. 4310-9, 2008 (PubMed). (Sample species: Human).

Liu, Qian, Sung, de Souza-Pinto, Zheng, Bogenhagen, Bohr, Wilson, Shen, Demple: "Removal of oxidative DNA damage via FEN1-dependent long-patch base excision repair in human cell mitochondria." in: Molecular and cellular biology, Vol. 28, Issue 16, pp. 4975-87, 2008 (PubMed). (Sample species: Human).

Szczesny, Tann, Longley, Copeland, Mitra: "Long patch base excision repair in mammalian mitochondrial genomes." in: The Journal of biological chemistry, Vol. 283, Issue 39, pp. 26349-56, 2008 (PubMed). (Sample species: Human).

Prasad, Liu, Deterding, Poltoratsky, Kedar, Horton, Kanno, Asagoshi, Hou, Khodyreva, Lavrik, Tomer, Yasui, Wilson: "HMGB1 is a cofactor in mammalian base excision repair." in: Molecular cell, Vol. 27, Issue 5, pp. 829-41, 2007 (PubMed). (Sample species: Human).

Kundu, Balusu, Jaiswal, Narayan: "Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways." in: Carcinogenesis, Vol. 28, Issue 10, pp. 2089-95, 2007 (PubMed).

Shibata, Nakamura: "Defective flap endonuclease 1 activity in mammalian cells is associated with impaired DNA repair and prolonged S phase delay." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 746-54, 2002 (PubMed). (Sample species: Human).

Kedar, Kim, Robertson, Hou, Prasad, Horton, Wilson: "Direct interaction between mammalian DNA polymerase beta and proliferating cell nuclear antigen." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 31115-23, 2002 (PubMed). (Sample species: Human).

Qiu, Li, Frank, Shen: "Cell cycle-dependent and DNA damage-inducible nuclear localization of FEN-1 nuclease is consistent with its dual functions in DNA replication and repair." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4901-8, 2001 (PubMed).