Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) antibody

Details for Product No. ABIN151110, Supplier: Log in to see
Antigen
  • fc39c11
  • wu:fc39c11
  • zgc:110210
  • AF9
  • YEATS3
  • 2210011H10Rik
  • 2610012I03Rik
  • 3830408D16Rik
  • Af9
  • D4Ertd321e
  • Af-9
  • myeloid/lymphoid or mixed lineage-leukemia translocation to 3 homolog (Drosophila)
  • myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 3
  • mllt3
  • MLLT3
  • Mllt3
Epitope
C-Term
31
14
10
8
6
5
4
4
4
4
3
2
2
1
1
1
1
1
Reactivity
Human
83
19
10
9
8
8
7
6
5
3
3
2
2
1
Host
Rabbit
80
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
71
37
30
29
20
16
6
4
2
Options
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Immunogen The immunogen recognized by this antibody maps to a region between residue 525 and the C-terminus (residue 568) of human ALL1 Fused Gene from Chromosome 9 Protein using the numbering given in entry NP_004520.1 (GeneID 4300).
Purification Immunogen affinity purified
Alternative Name AF9 (MLLT3 Antibody Abstract)
Background Gene Symbol: MLLT3
Gene ID 4300
UniProt P42568
Research Area Transcription Factors, Chromatin and Nuclear Signaling
Application Notes Western Blot 1:5000-1:15000, Immunohistochemistry 1:500 - 1:2000, Immunocytochemistry/Immunofluorescence 1:50- 1:500, Immunoprecipitation 1-4 μg/mg of lysate, Immunohistochemistry-Paraffin 1:500-1:2000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia (Trithorax Homolog, Drosophila), Translocated To, 3 (MLLT3) (C-Term) antibody (ABIN151110) Detection of Human AF9 by Western Blot and Immunoprecipitation. Samples: Whole cell l...
Product cited in: Qiao, Wang, Wang, Li, Yu, Yang, Song, Xu, Chin, Jing: "AF9 promotes hESC neural differentiation through recruiting TET2 to neurodevelopmental gene loci for methylcytosine hydroxylation." in: Cell discovery, Vol. 1, pp. 15017, 2016 (PubMed). Method employed by authors: Immunoprecipitation (IP)

Malik, Hemenway: "CBX8, a component of the Polycomb PRC1 complex, modulates DOT1L-mediated gene expression through AF9/MLLT3." in: FEBS letters, Vol. 587, Issue 18, pp. 3038-44, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Lin, Hemenway: "Hsp90 directly modulates the spatial distribution of AF9/MLLT3 and affects target gene expression." in: The Journal of biological chemistry, Vol. 285, Issue 16, pp. 11966-73, 2010 (PubMed).