BCL2 Binding Component 3 (BBC3) (AA 2-16) antibody

Details for Product No. ABIN151174
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Antigen
Epitope
AA 2-16
(63), (38), (12), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(201), (54), (54), (12), (12), (3)
Host
Rabbit
(166), (40), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(194), (69), (44), (38), (26), (20), (10), (4), (4), (2)
Pubmed 4 references available
Quantity 0.05 mg
Shipping to United States (Change)
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Catalog No. ABIN151174
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Immunogen Synthetic peptide: ARARQEGSSPEPVEG, corresponding to amino acids 2-16 of Human PUMA.
Specificity PUMA
Cross-Reactivity (Details) Based on sequence similarity, is expected to react with: Mouse.
Purification affinity purified
Alternative Name PUMA
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressorprotein induces apoptosis through transcriptional activation of several genes. A novelp53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in humanand mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family membersincluding Bax and Noxa, which are also transcriptional targets of p53. The PUMA geneencodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMAproteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release andapoptosis in response to p53. PUMA may be a direct mediator of p53-inducedapoptosis. Alternate Names: anti-p53 Upregulated Modulator of Apoptosis antibody, anti-Bcl-2 binding component 3antibody, anti-JFY-1 antibody.
Gene Symbol: BBC3
Gene ID 27113
Research Area Apoptosis/Necrosis
Application Notes This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Recommended dilutions: Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 10 µg/mL, Western Blot 2 µg/mL-4 µg/mL
Restrictions For Research Use only
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-BCL2 Binding Component 3 (BBC3) (AA 2-16) antibody PUMA ? staining of human breast carcinoma with anti-PUMA (NT) at 10 ?g/ml. Formalin fixed paraffin processed tissue.
General Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Stankovic, Hubank, Cronin et al.: "Microarray analysis reveals that TP53- and ATM-mutant B-CLLs share a defect in activating proapoptotic responses after DNA damage but are distinguished by major differences in activating prosurvival responses." in: Blood, Vol. 103, Issue 1, pp. 291-300, 2003 (PubMed).

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