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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
BCL2 Binding Component 3 (BBC3) (N-Term) antibody
|Synonyms||JFY1, PUMA, PUMA/JFY1, BBC3|
Alternatives Western Blotting (WB)
|4 references available|
|Price||Product not available in this region.|
|Immunogen||Synthetic peptide: ARARQEGSSPEPVEG, corresponding to amino acids 2-16 ofHuman PUMA.|
|Cross-Reactivity||Human, Mouse (Murine), Rat (Rattus)|
|Description||Apoptosis is related to many diseases and development. The p53 tumor-suppressorprotein induces apoptosis through transcriptional activation of several genes. A novelp53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in humanand mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family membersincluding Bax and Noxa, which are also transcriptional targets of p53. The PUMA geneencodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMAproteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release andapoptosis in response to p53. PUMA may be a direct mediator of p53-inducedapoptosis. Alternate Names: anti-p53 Upregulated Modulator of Apoptosis antibody, anti-Bcl-2 binding component 3antibody, anti-JFY-1 antibody.|
|Specificity||Species Reactivity: Cross-reacts with Human. Expected to cross-react with Mouse (100% identical),Rat(100% identical) due to sequence homology. Not yet tested in other species.|
|Application Notes||Positive Controls: K562 lysate|
|Purification||Aff - Purified|
|Buffer||PBS with 0.02% sodium azide|
|Storage||Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.|
|Restrictions||For Research Use only|
|PUMA ? staining of human breast carcinoma with anti-PUMA (NT) at 10 ?g/ml. Formalin fixed paraffin processed tissue.|
Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).
Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).
Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).
Stankovic, Hubank, Cronin et al.: "Microarray analysis reveals that TP53- and ATM-mutant B-CLLs share a defect in activating proapoptotic responses after DNA damage but are distinguished by major differences in activating prosurvival responses." in: Blood, Vol. 103, Issue 1, pp. 291-300, 2003 (PubMed).
|Hosts||Rabbit (63), Mouse (9), Goat (2)|
|Reactivities||Human (71), Mouse (Murine) (28), Rat (Rattus) (24), Cow (Bovine) (18), Dog (Canine) (18), Pig (Porcine) (18)|
|Applications||Western Blotting (WB) (60), ELISA (20), Immunofluorescence (IF) (17), Immunohistochemistry (IHC) (10), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoprecipitation (IP) (3), Flow Cytometry (FACS) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (14), N-Term (8), AA 76-170 (4), Internal Region (2), AA 134-154 (1), AA 2-16 (1), BH3 Domain (1), Center (1)|