BCL2 Binding Component 3 (BBC3) (N-Term) antibody

Antigen: BCL2 Binding Component 3 (BBC3)

Synonyms: JFY1, PUMA, PUMA/JFY1, BBC3

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN151174

Additional Information

Alternative name: PUMA

Gene ID: 27113

Immunogen: Synthetic peptide: ARARQEGSSPEPVEG, corresponding to amino acids 2-16 ofHuman PUMA.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Apoptosis is related to many diseases and development. The p53 tumor-suppressorprotein induces apoptosis through transcriptional activation of several genes. A novelp53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in humanand mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family membersincluding Bax and Noxa, which are also transcriptional targets of p53. The PUMA geneencodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b (1). PUMAproteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release andapoptosis in response to p53. PUMA may be a direct mediator of p53-inducedapoptosis. Alternate Names: anti-p53 Upregulated Modulator of Apoptosis antibody, anti-Bcl-2 binding component 3antibody, anti-JFY-1 antibody.

Specificity: Species Reactivity: Cross-reacts with Human. Expected to cross-react with Mouse (100% identical),Rat(100% identical) due to sequence homology. Not yet tested in other species.

Application Details

Application Notes: Positive Controls: K562 lysate

Purification: Aff - Purified

Buffer: PBS with 0.02% sodium azide

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Research Area: Apoptosis/Necrosis

Restrictions: For Research Use only

Images

PUMA ? staining of human breast carcinoma with anti-PUMA (NT) at 10 ?g/ml. Formalin fixed paraffin processed tissue.

Publications

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Stankovic, Hubank, Cronin et al.: "Microarray analysis reveals that TP53- and ATM-mutant B-CLLs share a defect in activating proapoptotic responses after DNA damage but are distinguished by major differences in activating prosurvival responses." in: Blood, Vol. 103, Issue 1, pp. 291-300, 2003 (PubMed).