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RAD51 Homolog D (S. Cerevisiae) (RAD51D) antibody

Details for Product No. ABIN151188
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Antigen
Synonyms BROVCA4, R51H3, RAD51L3, TRAD, ATRAD51D, F24B9.14, F24B9_14, SSN1, SUPPRESOR OF SNI1, homolog of RAD51 D, CG6318, Dm CG6318, Dmel\\CG6318, RAD51D, Rad51d, hmm140104
Reactivity
Human
(44), (3), (3), (1)
Host
Rabbit
(28), (13), (5)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(41), (17), (7), (5), (3), (2), (1), (1)
Pubmed 5 references available
Quantity 0.1 mL
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Catalog No. ABIN151188
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Immunogen A peptide from human Rad51D (homologue 4).
Specificity This is specific for hRAD51D. There is no cross-reactivity observed with XRCC2, XRCC3, RAD51, RAD51B, or RAD51C.
No Cross-Reactivity Mouse (Murine)
Purification affinity purified
Alternative Name RAD51L3
Background Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B,RAD51C, and RAD51D. Each of these homologues interacts with one or more of theother four, with all of the proteins involved in one complex or multiple smallercomplexes. RAD51D interacts with XRCC2 and RAD51C. RAD51D',s expressionpattern and sequence similarity to other RAD51 family members most likely makes itpart of a complex of proteins required for DNA repair and meiotic recombination. Alternate Names: anti-RAD51L3 antibody, anti-RAD51-like 3 (S. cerevisiae) antibody, anti-TRAD antibody,anti-R51H3 antibody, anti-HsTRAD antibody, anti-RAD51D antibody, anti-recombinationrepair protein antibody, anti-DNA repair protein RAD51 homolog 4 antibody.
Gene Symbol: RAD51D
Gene ID 5892, 19364
UniProt O75771
Application Notes This Rad51D antibody is useful for Western blot, where it recognizes a band at 35 kDa representing Rad51D (H3 splice variant). Immunocytochemistry/Immunofluorescence and Immunoprecipitation were reported in scientific literature.
Recommended dilutions: Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Western Blot 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1.9 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
anti-RAD51 Homolog D (S. Cerevisiae) (RAD51D) antibody Western blot detection of Rad51D (35 kDa) from Hela S3 cell extract using ABIN151188.
General Schild, Lio, Collins et al.: "Evidence for simultaneous protein interactions between human Rad51 paralogs." in: The Journal of biological chemistry, Vol. 275, Issue 22, pp. 16443-9, 2000 (PubMed).

Hinz, Tebbs, Wilson et al.: "Repression of mutagenesis by Rad51D-mediated homologous recombination." in: Nucleic acids research, Vol. 34, Issue 5, pp. 1358-68, 2006 (PubMed).

Wiese, Hinz, Tebbs et al.: "Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination." in: Nucleic acids research, Vol. 34, Issue 9, pp. 2833-43, 2006 (PubMed).

Chattopadhyay, Das, Maiti et al.: "Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1." in: Molecular and cellular biology, Vol. 28, Issue 23, pp. 7066-80, 2008 (PubMed).

Shammas, Shmookler Reis, Koley et al.: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN151188
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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