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HDAC3 antibody (Histone Deacetylase 3)

Details for Product anti-HDAC3 Antibody No. ABIN151195, Supplier: Log in to see
Antigen
  • CG2128
  • DHDAC3
  • DmHDAC3
  • Dmel\\CG2128
  • Dromel_CG2128_FBtr0078767_hdac3_mORF
  • HDAC
  • HDAC3
  • Hdac 3
  • dHDAC3
  • dmHDA402
  • hdac3
  • HDm
  • hd3
  • RPD3
  • XRpd3
  • rpd3-2
  • HD3
  • RPD3-2
  • AW537363
  • zgc:55927
  • Histone deacetylase 3
  • histone deacetylase 3
  • histone deacetylase 3-like
  • Hdac3
  • hdac3
  • CNL05260
  • HDAC3
  • LOC100354595
Alternatives
anti-Human HDAC3 antibody for Western Blotting
Reactivity
Human
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110
100
14
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5
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2
2
1
1
Host
Rabbit
173
67
2
Clonality
Polyclonal
Conjugate
This HDAC3 antibody is un-conjugated
7
6
6
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
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94
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62
45
29
26
22
13
9
4
3
3
1
1
1
Options
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Immunogen This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 2-17 of human HDAC3.
Specificity In HeLa, a 50 kDa band is observed.
Purification Protein G purified
Alternative Name HDAC3 (HDAC3 Antibody Abstract)
Background Gene Symbol: HDAC3
Gene ID 8841
Research Area DNA/RNA, Transcription Factors, Wnt, Cancer, Hypertrophy
Pathways Neurotrophin Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation
Application Notes Western Blot 2 - 5 μg/mL, Simple Western 1:25, Chromatin Immunoprecipitation 1:20-1:1000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500In HeLa, a 50 kDa band is observed. In Simple Western only 10 - 15 μL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-HDAC3 antibody (Histone Deacetylase 3) (ABIN151195) anti-Histone Deacetylase 3 (HDAC3) antibody
Simple Western (SimWes) image for anti-HDAC3 antibody (Histone Deacetylase 3) (ABIN151195) Simple Western: HDAC3 Antibody [ABIN151195] - Simple Western lane view shows a specif...
Immunofluorescence (IF) image for anti-HDAC3 antibody (Histone Deacetylase 3) (ABIN151195) Immunocytochemistry/Immunofluorescence: HDAC3 Antibody [ABIN151195] - HDAC3 antibody ...
Product cited in: Baek, Park, Rhim, Park, Kim, Kim, Nam: "Immunohistochemical Characterization of Histone Deacetylase as a Potential Prognostic Marker and Therapeutic Target in Endometrial Stromal Sarcoma." in: Anticancer research, Vol. 36, Issue 5, pp. 2527-34, 2016 (PubMed). (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Mano, Suzuki, Tsuji, Iwata: "Differential effect of HDAC3 on cytoplasmic and nuclear huntingtin aggregates." in: PLoS ONE, Vol. 9, Issue 11, pp. e111277, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Wilson, Byun, Nasser, Murray, Ayyanar, Arango, Figueroa, Melnick, Kao, Augenlicht, Mariadason: "HDAC4 promotes growth of colon cancer cells via repression of p21." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4062-75, 2008 (PubMed).

Jiang, Zhou, Fan, Heung, Xue, Liu, Kirschbaum, Yen: "A sequential treatment of depsipeptide followed by 5-azacytidine enhances Gadd45beta expression in hepatocellular carcinoma cells." in: Anticancer research, Vol. 27, Issue 6B, pp. 3783-9, 2008 (PubMed). (Sample species: Human). Further details: Western Blotting

Wilson, Byun, Popova, Murray, LItalien, Sowa, Arango, Velcich, Augenlicht, Mariadason: "Histone deacetylase 3 (HDAC3) and other class I HDACs regulate colon cell maturation and p21 expression and are deregulated in human colon cancer." in: The Journal of biological chemistry, Vol. 281, Issue 19, pp. 13548-58, 2006 (PubMed).

Imbriano, Gurtner, Cocchiarella, Di Agostino, Basile, Gostissa, Dobbelstein, Del Sal, Piaggio, Mantovani: "Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters." in: Molecular and cellular biology, Vol. 25, Issue 9, pp. 3737-51, 2005 (PubMed). Further details: Chromatin Immunoprecipitation

Caretti, Salsi, Vecchi, Imbriano, Mantovani: "Dynamic recruitment of NF-Y and histone acetyltransferases on cell-cycle promoters." in: The Journal of biological chemistry, Vol. 278, Issue 33, pp. 30435-40, 2003 (PubMed). Further details: Chromatin Immunoprecipitation

Cress, Seto: "Histone deacetylases, transcriptional control, and cancer." in: Journal of cellular physiology, Vol. 184, Issue 1, pp. 1-16, 2000 (PubMed).

Background publications Nakayama, Takami: "Participation of histones and histone-modifying enzymes in cell functions through alterations in chromatin structure." in: Journal of biochemistry, Vol. 129, Issue 4, pp. 491-9, 2001 (PubMed).