Histone Deacetylase 3 (HDAC3) antibody

Antigen: Histone Deacetylase 3 (HDAC3)

Synonyms: HD3, RPD3, RPD3-2, AW537363, DHDAC3, DmHDAC3, HDAC, HDAC3, dmHDA402, DmelCG2128, CG2128, MGC55927, zgc:55927, hdac3, HDm, hd3, XRpd3, rpd3-2, DKFZp469I1039

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN151195

Additional Information

Alternative name: HDAC3

Gene ID: 8841

Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptidecorresponding to amino acids 2-17 of human HDAC3.

Cross-Reactivity: Human

Description: Histone deacetylase (HDAC) and histone acetyltransferase (HAT) are enzymes thatregulate transcription by selectively deacetylating or acetylating the eta-amino groups oflysines located near the amino termini of core histone proteins (1). Eight members ofHDAC family have been identified in the past several years (2,3). These HDAC familymembers are divided into two classes, I and II. Class I of the HDAC family comprisesfour members, HDAC-1, 2, 3, and 8, each of which contains a deacetylase domainexhibiting from 45 to 93% identity in amino acid sequence. Class II of the HDAC familycomprises HDAC-4, 5, 6, and 7, the molecular weights of which are all about twofoldlarger than those of the class I members, and the deacetylase domains are presentwithin the C-terminal regions, except that HDAC-6 contains two copies of the domain,one within each of the N-terminal and C-terminal regions. Human HDAC-1, 2 and 3were expressed in various tissues, but the others (HDAC-4, 5, 6, and 7) showedtissue-specific expression patterns (3). These results suggested that each member ofthe HDAC family exhibits a different, individual substrate specificity and function in vivo. Alternate Names: anti-HD3 antibody, anti-RPD3 antibody, anti-RPD3-2 antibody.

Specificity: In HeLa, a 50 kDa band is observed. Species Reactivity: Cross-reacts with Human. Expected to cross-react with Mouse (100% identity withimmunogen), Rat (100% identity with immunogen) and Chicken (100% identity withimmunogen) due to sequence homology. Not yet tested in other species.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1-2ug/ml

Concentration: 0.5 mg/ml

Purification: Purified

Buffer: PBS containing 0.2% gelatin. Preservative: Sodium Azide.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Cancer, Hypertrophy, DNA/RNA, Transcription Factors

Restrictions: For Research Use only

Images

anti-Histone Deacetylase 3 (HDAC3) antibody

Publications

Cress, Seto: "Histone deacetylases, transcriptional control, and cancer." in: Journal of cellular physiology, Vol. 184, Issue 1, pp. 1-16, 2000 (PubMed).

Nakayama, Takami: "Participation of histones and histone-modifying enzymes in cell functions through alterations in chromatin structure." in: Journal of biochemistry, Vol. 129, Issue 4, pp. 491-9, 2001 (PubMed).

Wilson, Byun, Nasser et al.: "HDAC4 promotes growth of colon cancer cells via repression of p21." in: Molecular biology of the cell, Vol. 19, Issue 10, pp. 4062-75, 2008 (PubMed).