GFP antibody (Green Fluorescent Protein) (Biotin)

Details for Product anti-GFP Antibody No. ABIN151200, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
639
5
5
2
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1
1
Host
Goat
328
171
79
41
16
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2
Clonality
Polyclonal
Conjugate
This GFP antibody is conjugated to Biotin
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27
14
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11
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2
2
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2
1
1
1
1
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1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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26
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2
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Options
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Immunogen Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria).
Isotype IgG
Specificity GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Purification Immunogen affinity purified
Alternative Name GFP
Background Gene Symbol: GFP
Research Area Tags/Labels
Application Notes Western Blot 1:2000-1:10000, ELISA 1:10000-1:50000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, ImmunofluorescenceGFP Antibody is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Success in IF staining reported by customer review.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Western Blot: GFP Antibody [Biotin] [ABIN151200] - Lane 1: HeLa cells. Lane 2: mock t...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Immunocytochemistry/Immunofluorescence: GFP Antibody [Biotin] [ABIN151200] - Tissue: ...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Immunocytochemistry/Immunofluorescence: GFP Antibody [Biotin] [ABIN151200] - IF analy...
Western Blotting (WB) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Western Blot: GFP Antibody [Biotin] [ABIN151200] - Lane 1: GFP. Lane 2: None. Load: 5...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Immunocytochemistry/Immunofluorescence: GFP Antibody [Biotin] [ABIN151200] - Staining...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Immunocytochemistry/Immunofluorescence: GFP Antibody [Biotin] [ABIN151200] - GFP-Biot...
Immunofluorescence (IF) image for anti-GFP antibody (Green Fluorescent Protein)  (Biotin) (ABIN151200) Immunocytochemistry/Immunofluorescence: GFP Antibody [Biotin] [ABIN151200] - Tissue: ...
Product cited in: Dominguez-Brauer, Hao, Elia, Fortin, Nechanitzky, Brauer, Sheng, Mana, Chio, Haight, Pollett, Cairns, Tworzyanski, Inoue, Reardon, Marques, Silvester, Cox, Wakeham, Yilmaz, Sabatini, van Es, Clevers et al.: "Mule Regulates the Intestinal Stem Cell Niche via the Wnt Pathway and Targets EphB3 for Proteasomal and Lysosomal Degradation. ..." in: Cell stem cell, Vol. 19, Issue 2, pp. 205-16, 2016 (PubMed). Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Edelblute, Heller, Malik, Heller: "Activated air produced by shielded sliding discharge plasma mediates plasmid DNA delivery to mammalian cells." in: Biotechnology and bioengineering, Vol. 112, Issue 12, pp. 2583-90, 2015 (PubMed).

Long, Svensson, Tsai, Zeng, Roh, Kong, Rao, Lou, Lokurkar, Baur, Castellot, Rosen, Spiegelman: "A smooth muscle-like origin for beige adipocytes." in: Cell metabolism, Vol. 19, Issue 5, pp. 810-20, 2014 (PubMed). Further details: Immunohistochemistry (Paraffin-embedded Sections)