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RAD51 Homolog C (S. Cerevisiae) (RAD51C) antibody

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Human, Monkey, Mouse (Murine)
(73), (3), (1)
(40), (17), (16)
Clonality (Clone)
Monoclonal ()
(4), (4), (3), (3), (3), (3), (1), (1), (1)
Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(68), (39), (12), (6), (4), (2), (2), (1), (1)
Pubmed 6 references available
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Quantity 0.1 mL
Shipping to United States ( )
Immunogen His-tagged human Rad51C, over-expressed in E. coli.
Clone 2H11-6
Isotype IgG1 kappa
Specificity Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Purification Protein G purified
Alternative Name RAD51C (RAD51C Antibody Abstract)
Background The Rad51 DNA repair protein is a key component of the double-strand break repairpathway and is essential for mitotic and meiotic recombination and genomic stability.Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C,and Rad51D. Each of these homologs interacts with one or more of the others, with allof the proteins involved in one complex, or with multiple smaller complexes. Alternate Names: anti-DNA repair protein Rad51 homolog 3 antibody, anti-Rad51 homolog C (S.cerevisiae) antibody, anti-Rad51L2 antibody, anti-Yeast Rad51 homolog 3 antibody.
Gene Symbol: RAD51C
Gene ID 5889, 114714
UniProt O43502
Pathways DNA Damage Repair
Application Notes This Rad51C (2H11/6) antibody is useful for Flow Cytometry and Western Blot. In WB, a band can be seen at ~40 kDa. Preliminary feedback has been negative for Immunofluorescence on 4% PFA-fixed human cell lines (H1299 and MCF7). Use in IHC-P was reported in the scientific literature (PMID: 23512992).
Recommended dilutions: Flow Cytometry 1 µg per million cells, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot 1:1000
Protocol Protocol specific for Rad51C Antibody Western Blot Protocol
1. Preparation of samples for loading ~50-80ug of sample containing laemmli loading dye (containing SDS) at 90 °C for ~2 minutes.
. Load sample onto a 10 % Tris-HCL gel and run for ~30 minutes at 200V (or until dye front reaches bottom of gel).
. Place gel in transfer buffer for 10 minutes (192mM Glycine, 25mM Tris-HCL, 20 % Methanol). Pre-soak two pieces of Whatman paper and PVDF, as well. NOTE: The PVDF should be soaked in CH3OH for ~ 1minute, rinsed in ddH20 and then placed in transfer buffer.
. Transfer the protein from the gel to the membrane using a semi-dry transfer apparatus. Run for 20 minutes at 20V.
. Block non-specific proteins with blocking buffer #1 (10mM Tris-HCL pH 8.0, 300mM NaCL, 0.025 % Tween 20) for 10 minutes. Then continue blocking in blocking buffer #2 (buffer #1 + 15 % nonfat dry milk) for an additional hour, gently rocking at room temperature (RT) or overnight at 4 degrees Celcius.
. Dilute the primary antibody (anti-Rad51C, NB 100-177) in antibody dilution buffer (blocking buffer #1 + 2 % milk).
. Wash the membrane briefly with some blocking buffer #1 and then add your diluted primary antibody.
. Incubate the primary for 1 hour at room temperature, gently rocking. Again this can be done overnight at 4 Celcius.
. Wash 3X with blocking buffer #1 for 10 minutes, each, gently rocking.
. Incubate the diluted secondary antibody (anti-mouse IgG conjugated to HRP), diluted in antibody dilution buffer, for 1 hour at room temperature, gently rocking.
. Wash 2X with blocking buffer #1 for 10 minutes, each, gently rocking. Wash 1X with blocking buffer #1 for 30 minutes, gently rocking.
. Develop membrane with your chemiluminescent substrate. NOTE: NIH 3T3 and HEK 293 whole cell extracts and mouse embryonic fibroblast cells have been used as positive controls for this antibody.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, pH 7.4, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Supplier Images
Western Blotting (WB) image for anti-RAD51 Homolog C (S. Cerevisiae) (RAD51C) antibody (ABIN151297) Rad51C detected in HEK293 lysate using ABIN151297. Photo courtesy of B.T. Bennett & K...
Product cited in: Shammas, Shmookler Reis, Koley et al.: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed).

Chan, Koritzinsky, Zhao et al.: "Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance." in: Cancer research, Vol. 68, Issue 2, pp. 605-14, 2008 (PubMed).

Kuznetsov, Pellegrini, Shuda et al.: "RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females." in: The Journal of cell biology, Vol. 176, Issue 5, pp. 581-92, 2007 (PubMed).

Fan, Kumaravel, Jalali et al.: "Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression." in: Cancer research, Vol. 64, Issue 23, pp. 8526-33, 2004 (PubMed).

Maacke, Hundertmark, Miska et al.: "Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen." in: Journal of cancer research and clinical oncology, Vol. 128, Issue 4, pp. 219-22, 2002 (PubMed).

Masson, Tarsounas, Stasiak et al.: "Identification and purification of two distinct complexes containing the five RAD51 paralogs." in: Genes & development, Vol. 15, Issue 24, pp. 3296-307, 2001 (PubMed).

Catalog No. ABIN151297

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