RAD51 Homolog C (S. Cerevisiae) (RAD51C) antibody

Antigen: RAD51 Homolog C (S. Cerevisiae) (RAD51C)

Synonyms: RAD51L2, MGC104277, Dm CG2412, DmelCG2412, CG2412, Rad51l2, zgc:101596, RAD51C, RGD1563765, F4L23.21, Rad51c

Clonality: Monoclonal (2H11-6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN151297

Additional Information

Alternative name: RAD51C

Gene ID: 5889, 114714

Immunogen: His-tagged human Rad51C, over-expressed in E. coli.

Cross-Reactivity: Human

Format: Ascites

Isotype: IgG1

Clone: 2H11-6

Description: The Rad51 DNA repair protein is a key component of the double-strand break repairpathway and is essential for mitotic and meiotic recombination and genomic stability.Five human Rad51 homologs have been identified: XRCC2, XRCC3, Rad51B, Rad51C,and Rad51D. Each of these homologs interacts with one or more of the others, with allof the proteins involved in one complex, or with multiple smaller complexes. Alternate Names: anti-DNA repair protein Rad51 homolog 3 antibody, anti-Rad51 homolog C (S.cerevisiae) antibody, anti-Rad51L2 antibody, anti-Yeast Rad51 homolog 3 antibody.

Specificity: This antibody is specific for Rad51C. Species Reactivity: ABIN151297 recognizes human and mouse Rad51C. Other species have not beentested.ABIN151297 does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 inWestern analysis. Localization: Nuclear.

Application Details

Application Notes: Suggested working dilutions: Western Blot 1:1000

Concentration: 1 mg/ml

Purification: Ascites

Buffer: PBS (physiological pH). Preservative: 0.02% sodium azide.

Storage: Store at 4C. Do not freeze.

Restrictions: For Research Use only

Images

Rad51C detected in HEK293 lysate using ABIN151297. Photo courtesy of B.T. Bennett & K. Knight, University of Massachusetts Medical School.

Publications

Masson, Tarsounas, Stasiak et al.: "Identification and purification of two distinct complexes containing the five RAD51 paralogs." in: Genes & development, Vol. 15, Issue 24, pp. 3296-307, 2001 (PubMed).

Maacke, Hundertmark, Miska et al.: "Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen." in: Journal of cancer research and clinical oncology, Vol. 128, Issue 4, pp. 219-22, 2002 (PubMed).

Fan, Kumaravel, Jalali et al.: "Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression." in: Cancer research, Vol. 64, Issue 23, pp. 8526-33, 2004 (PubMed).

Kuznetsov, Pellegrini, Shuda et al.: "RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females." in: The Journal of cell biology, Vol. 176, Issue 5, pp. 581-92, 2007 (PubMed).

Chan, Koritzinsky, Zhao et al.: "Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance." in: Cancer research, Vol. 68, Issue 2, pp. 605-14, 2008 (PubMed).

Shammas, Shmookler Reis, Koley et al.: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed).