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RAD51C antibody (RAD51 Homolog C (S. Cerevisiae))

Details for Product anti-RAD51C Antibody No. ABIN151297, Supplier: Log in to see
Antigen
  • ATRAD51C
  • BROVCA3
  • CG2412
  • Dm CG2412
  • Dmel\\CG2412
  • F4L23.21
  • FANCO
  • R51H3
  • Rad51c
  • RAD51C
  • RAD51L2
  • Rad51l2
  • RAS associated with diabetes protein 51C
  • RGD1563765
  • zgc:101596
Alternatives
anti-Human RAD51C antibody for Western Blotting
Reactivity
Human, Mouse (Murine), Primate, Yeast
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19
17
17
3
1
Host
Mouse
40
38
15
Clonality (Clone)
Monoclonal ()
Conjugate
This RAD51C antibody is un-conjugated
5
5
5
5
4
4
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
88
43
26
19
19
13
5
2
2
1
Supplier
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Immunogen His-tagged human Rad51C, over-expressed in E. coli. [UniProt# O43502]
Clone 2H11-6
Isotype IgG1 kappa
Specificity Does not cross-react with Rad51B, Rad51D, Rad51, XRCC2, or XRCC3 in Western analysis.
Purification Protein G purified
Alternative Name Rad51C (RAD51C Antibody Abstract)
Background Gene Symbol: RAD51C
Molecular Weight Theoretical MW: 40 kDa
Gene ID 5889, 114714
UniProt O43502
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Pathways DNA Damage Repair
Application Notes Western Blot 1:1000, Simple Western 1:100, Flow Cytometry 1 μg per million cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for Rad51C Antibody Western Blot Protocol
1. Preparation of samples for loading ~50-80ug of sample containing laemmli loading dye (containing SDS) at 90 °C for ~2 minutes.
. Load sample onto a 10 % Tris-HCL gel and run for ~30 minutes at 200V (or until dye front reaches bottom of gel).
. Place gel in transfer buffer for 10 minutes (192mM Glycine, 25mM Tris-HCL, 20 % Methanol). Pre-soak two pieces of Whatman paper and PVDF, as well. NOTE: The PVDF should be soaked in CH3OH for ~ 1minute, rinsed in ddH20 and then placed in transfer buffer.
. Transfer the protein from the gel to the membrane using a semi-dry transfer apparatus. Run for 20 minutes at 20V.
. Block non-specific proteins with blocking buffer #1 (10mM Tris-HCL pH 8.0, 300mM NaCL, 0.025 % Tween 20) for 10 minutes. Then continue blocking in blocking buffer #2 (buffer #1 + 15 % nonfat dry milk) for an additional hour, gently rocking at room temperature (RT) or overnight at 4 degrees Celcius.
. Dilute the primary antibody (anti-Rad51C, NB 100-177) in antibody dilution buffer (blocking buffer #1 + 2 % milk).
. Wash the membrane briefly with some blocking buffer #1 and then add your diluted primary antibody.
. Incubate the primary for 1 hour at room temperature, gently rocking. Again this can be done overnight at 4 Celcius.
. Wash 3X with blocking buffer #1 for 10 minutes, each, gently rocking.
. Incubate the diluted secondary antibody (anti-mouse IgG conjugated to HRP), diluted in antibody dilution buffer, for 1 hour at room temperature, gently rocking.
. Wash 2X with blocking buffer #1 for 10 minutes, each, gently rocking. Wash 1X with blocking buffer #1 for 30 minutes, gently rocking.
. Develop membrane with your chemiluminescent substrate. NOTE: NIH 3T3 and HEK 293 whole cell extracts and mouse embryonic fibroblast cells have been used as positive controls for this antibody.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, pH 7.4
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.
Supplier Images
Western Blotting (WB) image for anti-RAD51C antibody (RAD51 Homolog C (S. Cerevisiae)) (ABIN151297) Rad51C detected in HEK293 lysate using ABIN151297. Photo courtesy of B.T. Bennett & K...
Western Blotting (WB) image for anti-RAD51C antibody (RAD51 Homolog C (S. Cerevisiae)) (ABIN151297) Western Blot: Rad51C Antibody (2H11/6) [ABIN151297] - Analysis of RAD51c in 1) Hela W...
Simple Western (SimWes) image for anti-RAD51C antibody (RAD51 Homolog C (S. Cerevisiae)) (ABIN151297) Simple Western: Rad51C Antibody (2H11/6) [ABIN151297] - Simple Western lane view show...
Flow Cytometry (FACS) image for anti-RAD51C antibody (RAD51 Homolog C (S. Cerevisiae)) (ABIN151297) Flow Cytometry: Rad51C Antibody (2H11/6) [ABIN151297] - Intracellular flow cytometric...
Product cited in: Park, Singh, Nassar, Zhang, Freund, Hanenberg, Meetei, Andreassen: "Breast cancer-associated missense mutants of the PALB2 WD40 domain, which directly binds RAD51C, RAD51 and BRCA2, disrupt DNA repair." in: Oncogene, Vol. 33, Issue 40, pp. 4803-12, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Min, Im, Yoon, Song, Nam, Hur, Kim, Lee, Han, Oh, Kim, OConnor, Kim, Bang: "RAD51C-deficient cancer cells are highly sensitive to the PARP inhibitor olaparib." in: Molecular cancer therapeutics, Vol. 12, Issue 6, pp. 865-77, 2013 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence

Jensen, Ozes, Kim, Estep, Kowalczykowski: "BRCA2 is epistatic to the RAD51 paralogs in response to DNA damage." in: DNA repair, Vol. 12, Issue 4, pp. 306-11, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Huang, Wang, Dhillon, Calses, Villegas, Mitchell, Tewari, Kemp, Taniguchi: "Systematic screen identifies miRNAs that target RAD51 and RAD51D to enhance chemosensitivity." in: Molecular cancer research : MCR, Vol. 11, Issue 12, pp. 1564-73, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Mao, Tian, Van Meter, Ke, Gorbunova, Seluanov: "Sirtuin 6 (SIRT6) rescues the decline of homologous recombination repair during replicative senescence." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 29, pp. 11800-5, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Wang, Huang, Calses, Kemp, Taniguchi: "MiR-96 downregulates REV1 and RAD51 to promote cellular sensitivity to cisplatin and PARP inhibition." in: Cancer research, Vol. 72, Issue 16, pp. 4037-46, 2012 (PubMed). (Sample species: Human). Further details: Western Blotting

Chun, Buechelmaier, Powell: "Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway." in: Molecular and cellular biology, Vol. 33, Issue 2, pp. 387-95, 2012 (PubMed). (Sample species: Human).

Sage, Gildemeister, Knight: "Discovery of a novel function for human Rad51: maintenance of the mitochondrial genome." in: The Journal of biological chemistry, Vol. 285, Issue 25, pp. 18984-90, 2010 (PubMed).

Shammas, Shmookler Reis, Koley, Batchu, Li, Munshi: "Dysfunctional homologous recombination mediates genomic instability and progression in myeloma." in: Blood, Vol. 113, Issue 10, pp. 2290-7, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Gildemeister, Sage, Knight: "Cellular redistribution of Rad51 in response to DNA damage: novel role for Rad51C." in: The Journal of biological chemistry, Vol. 284, Issue 46, pp. 31945-52, 2009 (PubMed).

Chan, Koritzinsky, Zhao, Bindra, Glazer, Powell, Belmaaza, Wouters, Bristow: "Chronic hypoxia decreases synthesis of homologous recombination proteins to offset chemoresistance and radioresistance." in: Cancer research, Vol. 68, Issue 2, pp. 605-14, 2008 (PubMed).

Kuznetsov, Pellegrini, Shuda, Fernandez-Capetillo, Liu, Martin, Burkett, Southon, Pati, Tessarollo, West, Donovan, Nussenzweig, Sharan: "RAD51C deficiency in mice results in early prophase I arrest in males and sister chromatid separation at metaphase II in females." in: The Journal of cell biology, Vol. 176, Issue 5, pp. 581-92, 2007 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Nagaraju, Odate, Xie, Scully: "Differential regulation of short- and long-tract gene conversion between sister chromatids by Rad51C." in: Molecular and cellular biology, Vol. 26, Issue 21, pp. 8075-86, 2006 (PubMed).

Bennett, Knight: "Cellular localization of human Rad51C and regulation of ubiquitin-mediated proteolysis of Rad51." in: Journal of cellular biochemistry, Vol. 96, Issue 6, pp. 1095-109, 2005 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Fan, Kumaravel, Jalali, Marrano, Squire, Bristow: "Defective DNA strand break repair after DNA damage in prostate cancer cells: implications for genetic instability and prostate cancer progression." in: Cancer research, Vol. 64, Issue 23, pp. 8526-33, 2004 (PubMed).

Liu, Masson, Shah, ORegan, West: "RAD51C is required for Holliday junction processing in mammalian cells." in: Science (New York, N.Y.), Vol. 303, Issue 5655, pp. 243-6, 2004 (PubMed). (Sample species: Human).

Maacke, Hundertmark, Miska, Voss, Kalthoff, Stürzbecher: "Autoantibodies in sera of pancreatic cancer patients identify recombination factor Rad51 as a tumour-associated antigen." in: Journal of cancer research and clinical oncology, Vol. 128, Issue 4, pp. 219-22, 2002 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry

French, Masson, Griffin, ORegan, West, Thacker: "Role of mammalian RAD51L2 (RAD51C) in recombination and genetic stability." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19322-30, 2002 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunoprecipitation,Immunofluorescence

Lambert, Lopez: "Inactivation of the RAD51 recombination pathway stimulates UV-induced mutagenesis in mammalian cells." in: Oncogene, Vol. 21, Issue 25, pp. 4065-9, 2002 (PubMed).

Masson, Tarsounas, Stasiak, Stasiak, Shah, McIlwraith, Benson, West: "Identification and purification of two distinct complexes containing the five RAD51 paralogs." in: Genes & development, Vol. 15, Issue 24, pp. 3296-307, 2001 (PubMed).