GFP antibody (Green Fluorescent Protein) (FITC)

Details for Product anti-GFP Antibody No. ABIN151299, Supplier: Log in to see
Antigen
  • green fluorescent protein
  • gfp
Reactivity
Aequorea victoria
641
23
16
5
5
2
2
1
1
Host
Goat
363
171
82
41
16
9
9
2
Clonality
Polyclonal
Conjugate
This GFP antibody is conjugated to FITC
33
31
27
14
14
14
12
11
11
10
8
7
7
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6
6
6
6
6
6
6
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5
4
4
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3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
FLISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Proximity Ligation Assay (PLA), Western Blotting (WB)
545
353
203
159
148
136
118
86
73
25
15
13
13
8
8
6
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4
4
4
3
3
3
3
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish (Aequorea victoria).
Isotype IgG
Specificity GFP antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Green Fluorescent Protein (Aequorea victoria).
Purification Immunogen affinity purified
Alternative Name GFP
Background Gene Symbol: GFP
Research Area Tags/Labels
Application Notes Western Blot 1:10000, Flow Cytometry 1:10-1:1000, Immunocytochemistry/Immunofluorescence 1:500-1:2500, Immunohistochemistry-Frozen, Proximity Ligation Assay, Fluorophore-linked immunosorbent assay 1:20000Use in Flow Cytometry reported in scientific literature (PMID: 23682015). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24056230) Use in Proximity Ligation Assay reported in scientific literature (PMID 24470503)
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (FITC) (ABIN151299) anti-Green Fluorescent Protein (GFP) antibody (FITC)
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (FITC) (ABIN151299) anti-Green Fluorescent Protein (GFP) antibody (FITC) (Image 2)
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (FITC) (ABIN151299) anti-Green Fluorescent Protein (GFP) antibody (FITC) (Image 3)
Immunofluorescence (IF) image for anti-Green Fluorescent Protein (GFP) antibody (FITC) (ABIN151299) Immunocytochemistry/Immunofluorescence: GFP Antibody [FITC] [ABIN151299] - Tissue: Sf...
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (FITC) (ABIN151299) Western Blot: GFP Antibody [FITC] [ABIN151299] - Lane 1: GFP. Lane 2: None. Load: 50 ...
Product cited in: Ntokou, Klein, Dontireddy, Becker, Bellusci, Richardson, Szibor, Braun, Morty, Seeger, Voswinckel, Ahlbrecht: "Characterization of the platelet-derived growth factor receptor-α-positive cell lineage during murine late lung development." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 309, Issue 9, pp. L942-58, 2015 (PubMed). Further details: Immunohistochemistry (Frozen Sections)

Doerner, Delling, Clapham: "Ion channels and calcium signaling in motile cilia." in: eLife, Vol. 4, 2015 (PubMed). (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Hauser, Grundström, Grundström: "Allelic exclusion of IgH through inhibition of E2A in a VDJ recombination complex." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 5, pp. 2460-70, 2014 (PubMed). Further details: Proximity Ligation Assay

Lampron, Pimentel-Coelho, Rivest: "Migration of bone marrow-derived cells into the central nervous system in models of neurodegeneration." in: The Journal of comparative neurology, Vol. 521, Issue 17, pp. 3863-76, 2013 (PubMed). Further details: Flow Cytometry

Freeman, Tao, Sun, Soonpaa, Rubart: "In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging." in: Journal of neuroscience methods, Vol. 221, pp. 48-61, 2013 (PubMed). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)

Hauser, Sveshnikova, Wallenius, Baradaran, Saarikettu, Grundström: "B-cell receptor activation inhibits AID expression through calmodulin inhibition of E-proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 4, pp. 1267-72, 2008 (PubMed).

Yee, Vitiello, Roper, Staversky, Wright, McGrath-Morrow, Maniscalco, Finkelstein, OReilly: "Type II epithelial cells are critical target for hyperoxia-mediated impairment of postnatal lung development." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 291, Issue 5, pp. L1101-11, 2006 (PubMed).

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