DMC1 Dosage Suppressor of Mck1 Homolog, Meiosis-Specific Homologous Recombination (Yeast) (DMC1) antibody

Details for Product No. ABIN151318, Supplier: Log in to see
Antigen
  • DMC1
  • zgc:136628
  • Dmc1h
  • Lim15
  • Mei11
  • sgdp
  • DCM1
  • DMC1H
  • LIM15
  • dJ199H16.1
  • DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
  • DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination
  • DNA meiotic recombinase 1
  • DMC1
  • dmc1
  • Dmc1
Reactivity
Human, Mouse (Murine)
121
47
41
19
2
2
2
2
1
1
1
1
1
1
Host
Mouse
91
31
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
99
29
28
19
17
15
13
11
11
1
Options
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Immunogen His-tagged human DMC1, overexpressed in E. coli [UniProt# Q14565]
Clone 1D12-4
Isotype IgG1
Purification Unpurified
Alternative Name DMC1 (DMC1 Antibody Abstract)
Background Gene Symbol: DMC1
Molecular Weight Theoretical MW: 37 kDa
Gene ID 11144
UniProt Q14565
Research Area DNA/RNA, Chromatin and Nuclear Signaling
Application Notes Western Blot 1:1000, ImmunoprecipitationThis DMC1 (1D12/4) antibody is useful for Western Blot, where a band can be seen at ~37 kDa for endogenous protein and ~40.5 kDa for recombinant his-tagged DMC1. Immunoprecipitation was reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for DMC1 Antibody Western BlotProcedure Guide for NB 100-181 Anti-hDMC-1Western Blot
1. Proteins are separated on an SDS-PAGE gel.
. Following the protein transfer, the membrane is blocked with 5 % NFDM in TBS-T.
. The membrane is then washed.
. NB 100-181 [anti-hDMC] is diluted 1:1,000 in dilution buffer [5 % NFDM in TBS-T] and incubated for 3 hours at room temperature, gently shaking.
. The membrane is then washed.
. Secondary antibody [HRP conjugated goat anti-mouse] is incubated for 45 minutes at room temperature, gently shaking.
. Develop membrane.NOTE: Mouse testis tissue extracts were used as a positive control for this antibody. MEF 161 and HeLa whole cell extracts were not shown to work in Western analysis.
Restrictions For Research Use only
Format Liquid
Buffer Ascites
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -80 °C,-20 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-DMC1 Dosage Suppressor of Mck1 Homolog, Meiosis-Specific Homologous Recombination (Yeast) (DMC1) antibody (ABIN151318) Western blot detection of DMC (37 kDa) from mouse testes using ABIN151318 (1:1000).
Western Blotting (WB) image for anti-DMC1 Dosage Suppressor of Mck1 Homolog, Meiosis-Specific Homologous Recombination (Yeast) (DMC1) antibody (ABIN151318) Western Blot: DMC1 Antibody (1D12/4) [ABIN151318] - Analysis of DMC1 on human testis ...
Product cited in: Masson, Davies, Hajibagheri, Van Dyck, Benson, Stasiak, Stasiak, West: "The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51." in: The EMBO journal, Vol. 18, Issue 22, pp. 6552-60, 2000 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunoprecipitation