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FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term)

Details for Product anti-FANCD2 Antibody No. ABIN151329, Supplier: Log in to see
Antigen
  • 2410150O07Rik
  • AU015151
  • BB137857
  • CG17269
  • CG31192
  • CG31194
  • Dmel\\CG17269
  • dmFANCD2
  • FA-D2
  • FA4
  • FACD
  • FAD
  • FAD2
  • FANCD
  • FANCD2
  • xfancd2
Epitope
N-Term
21
16
13
5
4
4
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Primate
113
57
37
11
11
3
2
Host
Rabbit
103
15
Clonality
Polyclonal
Conjugate
This FANCD2 antibody is un-conjugated
5
5
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Simple Western (SimWes), Western Blotting (WB)
80
51
30
29
23
23
21
12
2
1
1
1
Supplier
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Immunogen Human FANCD2 fusion protein (N-terminal fragment). [Swiss-Prot #Q9BXW9]
Specificity This antibody is specific for FANCD2.
Purification Immunogen affinity purified
Alternative Name FANCD2 (FANCD2 Antibody Abstract)
Background Gene Symbol: FANCD2
Gene ID 2177, 211651
UniProt Q9BXW9
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes Western Blot 1:10000-1:20000, Simple Western 1:25, Flow Cytometry, Immunohistochemistry 2.5-5.0 μg/mL, Immunocytochemistry/Immunofluorescence 1:200-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 2.5-5.0 μg/mL
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for FANCD2 Antibody Western Blot
1. Proteins are separated on a 7.5 % SDS-PAGE gel.
. Following the protein transfer, the membrane is blocked with TBST + 3 % milk.
. Anti-FANCD2 [cat# NB 100-182] is diluted in blocking buffer and incubated for 1 hour at room temperature, gently shaking.
. The membrane is then washed, 3 times with TBST, 5 minutes each.
. Secondary antibody is incubated for 1 hour at room temperature, gently shaking.
. The membrane is then washed, 3 times with TBST, 5 minutes each.
. Membrane is developed using ECL reagents. Visualization after a 30 second -2 minute exposure. NOTE: HeLa whole cell extracts [cat# NB 800-PC1] were used as positive control for this antibodyImmunoprecipitation 4ul for 1 mg of whole cell extract prepared by lysis in either standard RIPA buffer or a mild buffer composed of 10 mM Tris/HCl pH 8, 150 mM NaCl, 2 mM MgCl2, 0.1 % Triton X-100 + inhibitors.Immunohistochemistry - FFPE sectionsI. DeparaffinizationA. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changesB. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous PeroxidaseA. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution: Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol. Use within 4 hours of preparation.B. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Supplier Images
Immunofluorescence (IF) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN151329) FancD2 colocalizes in vivo with another protein in SiHa cells after cell exposure to ...
Immunofluorescence (IF) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN151329) FancD2 colocalizes in vivo with another protein in U2OS cells after cell exposure to ...
Western Blotting (WB) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN151329) FANCD2 in HeLa WCE using ABIN151329 (lot C).
Simple Western (SimWes) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN151329) Simple Western: FANCD2 Antibody [ABIN151329] - Simple Western lane view shows a speci...
Western Blotting (WB) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN151329) Western Blot: FANCD2 Antibody [ABIN151329] - Analysis of FANCD2 in human PD20 cells. ...
Immunohistochemistry (IHC) image for anti-FANCD2 antibody (Fanconi Anemia, Complementation Group D2) (N-Term) (ABIN151329) Immunohistochemistry: FANCD2 Antibody [ABIN151329] - Analysis using the HRP conjugate...
Product cited in: Panneerselvam, Wang, Zhang, Che, Yu, Fei: "BLM promotes the activation of Fanconi Anemia signaling pathway." in: Oncotarget, Vol. 7, Issue 22, pp. 32351-61, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Wang, Gao, Lu, Tatetsu, Williams, Müller, Cui, Chai: "Leukemic survival factor SALL4 contributes to defective DNA damage repair." in: Oncogene, Vol. 35, Issue 47, pp. 6087-6095, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Maeda, Froning, Brents, Rose, Thamm, Kato: "Intrinsic Radiosensitivity and Cellular Characterization of 27 Canine Cancer Cell Lines." in: PLoS ONE, Vol. 11, Issue 6, pp. e0156689, 2016 (PubMed). (Sample species: Dog (Canine)). Further details: Western Blotting

Chun, Hwang, Kim, Goh, Kim, Lee: "Aurora A kinase is required for activation of the Fanconi anemia/BRCA pathway upon DNA damage." in: FEBS open bio, Vol. 6, Issue 7, pp. 782-90, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence

Root, Larsen, Komosa, Al-Azri, Li, Bazett-Jones, Stephen Meyn: "FANCD2 limits BLM-dependent telomere instability in the alternative lengthening of telomeres pathway." in: Human molecular genetics, 2016 (PubMed).

Fu, Begum, Jordan, He, Overbeek: "Dearth and Delayed Maturation of Testicular Germ Cells in Fanconi Anemia E Mutant Male Mice." in: PLoS ONE, Vol. 11, Issue 8, pp. e0159800, 2016 (PubMed). Method employed by authors: Flow Cytometry (FACS)

Kais, Rondinelli, Holmes, OLeary, Kozono, DAndrea, Ceccaldi: "FANCD2 Maintains Fork Stability in BRCA1/2-Deficient Tumors and Promotes Alternative End-Joining DNA Repair." in: Cell reports, Vol. 15, Issue 11, pp. 2488-99, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Bezine, Malaisé, Loeuillet, Chevalier, Boutet-Robinet, Salles, Mirey, Vignard: "Cell resistance to the Cytolethal Distending Toxin involves an association of DNA repair mechanisms." in: Scientific reports, Vol. 6, pp. 36022, 2016 (PubMed). Method employed by authors: Western Blotting (WB)

Michl, Zimmer, Buffa, McDermott, Tarsounas: "FANCD2 limits replication stress and genome instability in cells lacking BRCA2." in: Nature structural & molecular biology, Vol. 23, Issue 8, pp. 755-7, 2016 (PubMed).

Sato, Shimomuki, Katsuki, Takahashi, Kobayashi, Ishiai, Miyoshi, Takata, Kurumizaka: "FANCI-FANCD2 stabilizes the RAD51-DNA complex by binding RAD51 and protects the 5'-DNA end." in: Nucleic acids research, Vol. 44, Issue 22, pp. 10758-10771, 2016 (PubMed).

Alavattam, Kato, Sin, Maezawa, Kowalski, Zhang, Pang, Andreassen, Namekawa: "Elucidation of the Fanconi Anemia Protein Network in Meiosis and Its Function in the Regulation of Histone Modifications." in: Cell reports, Vol. 17, Issue 4, pp. 1141-1157, 2016 (PubMed).

Vuono, Mukherjee, Vierra, Adroved, Hodson, Deans, Howlett: "The PTEN phosphatase functions cooperatively with the Fanconi anemia proteins in DNA crosslink repair." in: Scientific reports, Vol. 6, pp. 36439, 2016 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Zhu, Yan, Li, Lin, Zhang, He, Zheng, Yang, Shao: "K63-linked ubiquitination of FANCG is required for its association with the Rap80-BRCA1 complex to modulate homologous recombination repair of DNA interstand crosslinks." in: Oncogene, Vol. 34, Issue 22, pp. 2867-78, 2015 (PubMed).

Duan, Gao, Aguila, Kalvala, Otterson, Villalona-Calero: "Fanconi anemia repair pathway dysfunction, a potential therapeutic target in lung cancer." in: Frontiers in oncology, Vol. 4, pp. 368, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Stoepker, Faramarz, Rooimans, van Mil, Balk, Velleuer, Ameziane, Te Riele, de Winter: "DNA helicases FANCM and DDX11 are determinants of PARP inhibitor sensitivity." in: DNA repair, Vol. 26, pp. 54-64, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Aarts, Bajrami, Herrera-Abreu, Elliott, Brough, Ashworth, Lord, Turner: "Functional Genetic Screen Identifies Increased Sensitivity to WEE1 Inhibition in Cells with Defects in Fanconi Anemia and HR Pathways." in: Molecular cancer therapeutics, Vol. 14, Issue 4, pp. 865-76, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Brem, Zhang, Xu, Karran: "UVA photoactivation of DNA containing halogenated thiopyrimidines induces cytotoxic DNA lesions." in: Journal of photochemistry and photobiology. B, Biology, Vol. 145, pp. 1-10, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Xie, Kim, Moreau, Puhalla, Garber, Al Abo, Takeda, DAndrea: "RNF4-mediated polyubiquitination regulates the Fanconi anemia/BRCA pathway." in: The Journal of clinical investigation, Vol. 125, Issue 4, pp. 1523-32, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Oka, Bekker-Jensen, Mailand: "Ubiquitin-like protein UBL5 promotes the functional integrity of the Fanconi anemia pathway." in: The EMBO journal, Vol. 34, Issue 10, pp. 1385-98, 2015 (PubMed). (Sample species: Human). Further details: Western Blotting

Zhang, Wilson, Mahmood Ali, Namekawa, Andreassen, Ruhikanta Meetei, Pang: "Loss of Faap20 Causes Hematopoietic Stem and Progenitor Cell Depletion in Mice Under Genotoxic Stress." in: Stem cells (Dayton, Ohio), Vol. 33, Issue 7, pp. 2320-30, 2015 (PubMed).