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Antibodies
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anti-Stage Specific Embryonic Antigen 3 (SSEA3) antibody
Stage Specific Embryonic Antigen 3 (SSEA3) antibody
| Antigen |
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| Clonality |
Monoclonal (MC-631) |
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Host
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Reactivity
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Conjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. |
ABIN151342 |
| Quantity |
0.1 ml (Variants) |
| Price |
Product not available in this region.
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| Shipping to |
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Alternative name
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SSEA3
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Gene ID
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93961
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Immunogen
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4-8 cell stage mouse embryos.
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Cross-Reactivity
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Human, Mouse (Murine)
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Format
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Ascites
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Isotype
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IgM
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Clone
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MC-631
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Description
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SSEA3 (stage-specific mouse embryonic antigen) is a cell-surface protein involved incell differentiation. Cell surface antigens are powerful tools for characterizing thefunction and expression of developmentally regulated cells during both embryogenesisand oncogenesis. Alternate Names: Anti-Stage specific embryonic antigen 3 antibody, Anti-Stage-specific embryonic antigen3 antibody.
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Specificity
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Species Reactivity: This antibody reacts with the Stage-specific embryonic antigen-3 (SSEA-3) that isexpressed upon the surface of human teratocarcinoma stem cells (EC), humanembryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivityis evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-3 isdown regulated following differentiation of human EC cells. In contrast, thedifferentiation of murine EC and ES cells may be accompanied by an increase inSSEA-3 expression.
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Application Notes
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Suggested working dilutions: flow cytometry / FACS analysis - starting at a range of 10-20 ug/mL is suggested.,immunofluorescence
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Purification
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Ascites
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Buffer
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Ascites. Preservative: 0.1% sodium azide.
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Storage
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Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Restrictions
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For Research Use only
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Henderson, Draper, Baillie et al.: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).
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Alternatives for antigen "Stage Specific Embryonic Antigen 3 (SSEA3)", type "Antibodies"
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Hosts
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Rabbit (19), Rat (1)
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Reactivities
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Human (20), Mouse (Murine) (19), Rat (Rattus) (19)
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Applications
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Immunofluorescence (IF) (17), ELISA (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1), Immunoprecipitation (IP) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)
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2013 BioMed Central Research Awards
