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Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody

Details for Product No. ABIN151342, Supplier: Log in to see
Antigen
Reactivity
Human, Mouse (Murine)
42
39
Host
Rat
42
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
37
24
22
20
17
16
16
16
2
2
2
1
1
Supplier
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Immunogen 4-8 cell stage mouse embryos.
Clone MC-631
Isotype IgM
Purification IgM purified
Alternative Name SSEA-3
Application Notes Western Blot 1:500, Flow Cytometry 10-20 μg/mL, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:50-1:100, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB100-1832 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Immunocytochemistry (ICC) image for anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody (ABIN151342) anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody
Immunofluorescence (IF) image for anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody (ABIN151342) Immunocytochemistry/Immunofluorescence: SSEA-3 Antibody (MC-631) [ABIN151342] - HeLa ...
Flow Cytometry (FACS) image for anti-Thiosulfate Sulfurtransferase, SSEA-3 (SSEA-3) antibody (ABIN151342) Flow Cytometry: SSEA-3 Antibody (MC-631) [ABIN151342] - Staining of NTERA-2 cells usi...
Product cited in: Skottman: "Derivation and characterization of three new human embryonic stem cell lines in Finland." in: In vitro cellular & developmental biology. Animal, Vol. 46, Issue 3-4, pp. 206-9, 2010 (PubMed).

Hakala, Rajala, Ojala, Panula, Areva, Kellomäki, Suuronen, Skottman: "Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines." in: Tissue engineering. Part A, Vol. 15, Issue 7, pp. 1775-85, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Matin, Walsh, Gokhale, Draper, Bahrami, Morton, Moore, Andrews: "Specific knockdown of Oct4 and beta2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells." in: Stem cells (Dayton, Ohio), Vol. 22, Issue 5, pp. 659-68, 2004 (PubMed).

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed). (Sample species: Human). Further details: Flow Cytometry

Przyborski: "Isolation of human embryonal carcinoma stem cells by immunomagnetic sorting." in: Stem cells (Dayton, Ohio), Vol. 19, Issue 6, pp. 500-4, 2001 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Kannagi, Cochran, Ishigami, Hakomori, Andrews, Knowles, Solter: "Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells." in: The EMBO journal, Vol. 2, Issue 12, pp. 2355-61, 1984 (PubMed). (Sample species: Human). Further details: Western Blotting