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Podocalyxin-Like (PODXL) antibody

Details for Product No. ABIN151343, Supplier: Log in to see
Antigen
  • PODXL
  • podxl
  • fc18d02
  • wu:fc18d02
  • Gp200
  • PC
  • PCLP
  • PCLP-1
  • AW121214
  • Ly102
  • Pclp1
  • podocalyxin
  • Gp135
  • PCLP1
  • cPCLP1
  • MEP21
  • Podxl
  • podocalyxin-like
  • si:dkey-146n1.1
  • podocalyxin-like protein 1
  • PODXL
  • si:dkey-146n1.1
  • LOC100614602
  • Podxl
  • LOC100512201
  • PCLP1
Reactivity
Human
272
89
82
62
3
2
2
2
1
1
1
1
Host
Mouse
158
107
21
18
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
17
17
15
12
10
9
7
7
7
7
7
6
5
4
3
3
3
3
3
3
2
2
2
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
195
152
87
86
85
72
47
38
35
5
4
4
3
2
2
1
Options
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Immunogen Human embryonal carcinoma cell line 2102Ep.
Clone TRA-1-81
Isotype IgM
No Cross-Reactivity Mouse (Murine)
Purification IgM purified
Alternative Name TRA-1-81 (PODXL Antibody Abstract)
Application Notes Western Blot 1:500, Flow Cytometry 1:10-1:1000, Immunohistochemistry 1:50-1:200, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:200, Immunohistochemistry-Frozen 1:50-1:200This TRA-1-81 antibody is useful for Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot. Flow Cytometry was reported in scientific literature.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol Specific for NB100-1833 Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
1. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Western Blot: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Western blot analysis of AB...
Flow Cytometry (FACS) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Indirect FCM analysis of ...
Immunofluorescence (IF) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Immunofluorescence st...
Flow Cytometry (FACS) image for anti-Podocalyxin-Like (PODXL) antibody (ABIN151343) Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Analysis using the Alexa ...
Product cited in: Rossbach, Hildebrand, El-Ahmad, Stachelscheid, Reinke, Kurtz: "Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector." in: Stem cell research, Vol. 16, Issue 2, pp. 314-7, 2016 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Hildebrand, Seemann, Kurtz, Hecht, Contzen, Gossen, Stachelscheid: "Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses." in: Cellular and molecular life sciences : CMLS, Vol. 72, Issue 23, pp. 4671-80, 2015 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Mizrak, Chikhovskaya, Sadri-Ardekani, van Daalen, Korver, Hovingh, Roepers-Gajadien, Raya, Fluiter, de Reijke, de la Rosette, Knegt, Belmonte, van der Veen, de Rooij, Repping, van Pelt: "Embryonic stem cell-like cells derived from adult human testis." in: Human reproduction (Oxford, England), Vol. 25, Issue 1, pp. 158-67, 2009 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Henderson, Draper, Baillie, Fishel, Thomson, Moore, Andrews: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Flow Cytometry,Immunofluorescence

Badcock, Pigott, Goepel, Andrews: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed). (Sample species: Human). Further details: Western Blotting