Podocalyxin-Like (PODXL) antibody

Details for Product No. ABIN151343
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Antigen
Synonyms Gp200, PC, PCLP, PCLP-1, Ly102, Pclp1, AW121214, podocalyxin, MEP21, Gp135, PCLP1, cPCLP1, Podxl, PODXL, podxl, fc18d02, wu:fc18d02
Reactivity
Human
(78), (15), (7), (1)
Host
Mouse
(52), (33), (8), (5), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (7), (3), (2), (2), (2), (2)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(76), (44), (25), (20), (15), (13), (13), (13), (4), (4), (1), (1)
Pubmed 2 references available
Catalog no. ABIN151343
Quantity 0.1 mL
Price
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Immunogen Human embryonal carcinoma cell line 2102Ep.
Clone TRA-1-81
Isotype IgM
Specificity Species Reactivity: This antibody reacts with TRA-1-81 antigen that is expressed upon the surface ofhuman teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and humanembryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells.This antibody recognizes antigens that are associated with a pericellular matrixproteoglycan. Localization: Cell Membrane.
Purification Ascites
Alternative Name TRA-1-81
Background TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigensare powerful tools for characterizing the function and expression of developmentallyregulated cells during both embryogenesis and oncogenesis.
Gene ID 5420
Application Notes Suggested working dilutions *Western Blot, flow cytometry / FACS analysis, immunohistochemistry,immunoprecipitation, immunofluorescence * Investigator should determine optimal working dilutions. This antibody may be useful for FACS, immunofluoresence, Western blot,immunoprecipitation, and immunohistochemistry. Other applications have not beentested.*The investigator should determine the optimal working dilution for a specific application. * Other applications have not been tested.
Restrictions For Research Use only
Format Liquid
Concentration 5.7 mg/ml
Buffer Preservative: 0.1% sodium azide.
Preservative Sodium azide
Storage -20 °C
Supplier Images
anti-Podocalyxin-Like (PODXL) antibody Western Blot: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Western blot analysis of ABIN151343 expression in NTERA-2 cl.D1 whole cell lysate.
anti-Podocalyxin-Like (PODXL) antibody (2) Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Indirect FCM analysis of NTERA-2 cl.D1 cells stained with ABIN151343, followed by PE-conjugated goat anti-mouse IgM.
anti-Podocalyxin-Like (PODXL) antibody (3) Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Immunofluorescence staining of methanol-fixed NTERA-2 cl.D1 cells showing membrane localization.
General Badcock, Pigott, Goepel et al.: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed).

Henderson, Draper, Baillie et al.: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).

Hosts (52), (33), (8), (5), (2)
Reactivities (78), (15), (7), (1)
Applications (76), (44), (25), (20), (15), (13), (13), (13), (4), (4), (1), (1)
Conjugates (8), (7), (3), (2), (2), (2), (2)
Epitopes (13), (7), (6), (6), (3), (2), (2), (2), (1), (1), (1)
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