Podocalyxin-Like (PODXL) antibody

Antigen: Podocalyxin-Like (PODXL)

Synonyms: PC, PCLP, Gp200, MGC138240, Ly102, Pclp1, PCLP-1, AW121214, podocalyxin, PODXL, podxl, fc18d02, wu:fc18d02

Clonality: Monoclonal (TRA-1-81)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN151343

Additional Information

Alternative name: TRA-1-81

Gene ID: 5420

Immunogen: Human embryonal carcinoma cell line 2102Ep.

Format: Ascites

Isotype: IgM

Clone: TRA-1-81

Description: TRA-1-81 is a cell-surface protein involved in cell differentiation. Cell surface antigensare powerful tools for characterizing the function and expression of developmentallyregulated cells during both embryogenesis and oncogenesis.

Specificity: Species Reactivity: This antibody reacts with TRA-1-81 antigen that is expressed upon the surface ofhuman teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and humanembryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells.This antibody recognizes antigens that are associated with a pericellular matrixproteoglycan. Localization: Cell Membrane.

Application Details

Application Notes: Suggested working dilutions *Western Blot, flow cytometry / FACS analysis, immunohistochemistry,immunoprecipitation, immunofluorescence * Investigator should determine optimal working dilutions. This antibody may be useful for FACS, immunofluoresence, Western blot,immunoprecipitation, and immunohistochemistry. Other applications have not beentested.*The investigator should determine the optimal working dilution for a specific application. * Other applications have not been tested.

Concentration: 5.7 mg/ml

Buffer: Preservative: 0.1% sodium azide.

Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Restrictions: For Research Use only

Images

Western Blot: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Western blot analysis of ABIN151343 expression in NTERA-2 cl.D1 whole cell lysate.

Flow Cytometry: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Indirect FCM analysis of NTERA-2 cl.D1 cells stained with ABIN151343, followed by PE-conjugated goat anti-mouse IgM.

Immunofluorescence: TRA-1-81 Antibody (TRA-1-81) [ABIN151343] - Immunofluorescence staining of methanol-fixed NTERA-2 cl.D1 cells showing membrane localization.

Publications

Badcock, Pigott, Goepel et al.: "The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan." in: Cancer research, Vol. 59, Issue 18, pp. 4715-9, 1999 (PubMed).

Henderson, Draper, Baillie et al.: "Preimplantation human embryos and embryonic stem cells show comparable expression of stage-specific embryonic antigens." in: Stem cells (Dayton, Ohio), Vol. 20, Issue 4, pp. 329-37, 2002 (PubMed).