Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (C-Term), (AA 500-542) antibody

Details for Product No. ABIN151381
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Antigen
Synonyms
AF001784, CG3747, Dglt-1, Dglut-1, Dmel\\CG3747, EAAT, EAAT1, dEAAT, dEAAT1, dEaat1, slc1a3, wu:fb98e05, wu:fd50b05, zgc:56661, zgc:77768, SLC1A3, GLAST, GluT-1, EA6, GLAST1, AI504299, B430115D02Rik,  ... show more
AF001784, CG3747, Dglt-1, Dglut-1, Dmel\\CG3747, EAAT, EAAT1, dEAAT, dEAAT1, dEaat1, slc1a3, wu:fb98e05, wu:fd50b05, zgc:56661, zgc:77768, SLC1A3, GLAST, GluT-1, EA6, GLAST1, AI504299, B430115D02Rik, Eaat1, GLAST-1, GLU-T, Gmt1, MGluT1 show less
Epitope
C-Term, AA 500-542
(32), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Rat (Rattus)
(47), (36), (22)
Host
Rabbit
(51), (9), (4), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(48), (23), (14), (10), (9), (7), (6), (4), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
Shipping to United States (Change)
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Catalog No. ABIN151381
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Immunogen A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542)
Cross-Reactivity (Details) Based upon 100% immunogen sequence similarity, this antibody is predicted to cross-react with Bovine also.
Purification affinity purified
Alternative Name SLC1A3 / EAAT1
Background Human excitatory amino acid transporters (EAATs) are members of a family of highaffinity sodium-dependent transporter molecules that regulate neurotransmitterconcentrations at the excitatory glutamatergic synapses of the mammalian centralnervous system. It is believed that these proteins reduce extracellular glutamateconcentration, thereby modulating synaptic signaling. In addition, EAATs may also beimportant for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressedin the frontal cortex, hippocampus and basal ganglia and is also reported to be found inheart, placenta, lung and striated muscle. EAAT1 shares some pharmacologicalsimilarities with EAAT3 and both are potent antagonists that appear to specifically blocktransport mediated by EAAT2.EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport byco-transporting sodium. EAAT1 is essential for terminating the postsynaptic action ofglutamate, by rapidly removing released glutamate from the synaptic cleft. Alternate Names: anti-GLAST antibody, anti-EA6 antibody,anti-EAAT1 antibody, anti-Excitatory amino acid transporter 1 antibody, anti-FLJ25094 antibody, anti-GLAST antibody,anti-GLAST1 antibody, anti-Glial high affinity glutamate transporter antibody,anti-High affinity neuronal glutamate transporter antibody, anti-Slc1a3 antibody,anti-Sodium dependent glutamate/aspartate transporter antibody.
Gene Symbol: SLC1A3
Gene ID 6507
UniProt P24942
Application Notes Recommended dilutions: ELISA 1:100-1:2000, Flow Cytometry 1:10-1:200, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry-Paraffin 1:50-1:500, Western Blot 2 µg/mL
Protocol Western blot Protocol for SLC1A3 antibody
Western Blot Protocol:
1. Perform SDS-PAGE on protein samples to be analyzed, loading 10-40 µg of total protein per lane.
. Electro-blot the proteins to a suitable membrane (PVDF or Nitrocellulose) according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain the membrane with Ponceau S (or a similar product) to assess transfer success. Mark molecular weight standards where appropriate.
. Thoroughly rinse the membrane of stain with TBST.
. Incubate the membrane in blocking buffer (5 % non-fat milk in TBST or 5 % BSA in TBST) as appropriate, for 60 minutes.
. Dilute the SLC1A3 primary antibody as appropriate in blocking buffer and incubate for 60 minute at room temperature to overnight at 4 degrees C with gently shaking.
. Wash the membrane in TBST three times for 10 minutes each.
. Incubate the membrane in the appropriate secondary antibody prepared in blocking buffer (as per manufacturer's instructions) and incubate for 60 minutes at room temperature.
. Wash the membrane in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
. Incubate the membrane in the appropriate detection reagent in accordance with the manufacturer's instructions and image the blot.Note: Tween-20 can be added to the blocking, wash and antibody dilution buffers to a final concentration of 0.05-0.1 %.Immunohistochemistry-Paraffin protocol for SLC1A3 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 60 minutes at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.
General Rothstein, Dykes-Hoberg, Pardo et al.: "Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate." in: Neuron, Vol. 16, Issue 3, pp. 675-86, 1996 (PubMed).

Schmitt, Asan, Pueschel et al.: "Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 1, pp. 1-10, 1997 (PubMed).

Kobayashi, Millhorn: "Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells." in: Journal of neurochemistry, Vol. 76, Issue 6, pp. 1935-48, 2001 (PubMed).

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