Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) antibody

Antigen: Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3)

Synonyms: EA6, EAAT1, GLAST, GLAST1, FLJ25094, Gmt1, Eaat1, GLU-T, GluT-1, MGluT1, GLAST-1, AI504299, B430115D02Rik, AF001784, CG3747, dEAAT, dEaat1, dEAAT1, Dglt-1, Dglut-1, DmelCG3747, EAAT, MGC140731, zgc:56661, zgc:77768, wu:fb98e05, wu:fd50b05, SLC1A3

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN151381

Additional Information

Alternative name: SLC1A3 / EAAT1

Immunogen: 20 residue C-terminal synthetic peptide (Rat).

Cross-Reactivity: Rat (Rattus)

Format: Serum

Description: Human excitatory amino acid transporters (EAATs) are members of a family of highaffinity sodium-dependent transporter molecules that regulate neurotransmitterconcentrations at the excitatory glutamatergic synapses of the mammalian centralnervous system. It is believed that these proteins reduce extracellular glutamateconcentration, thereby modulating synaptic signaling. In addition, EAATs may also beimportant for the prevention of glutamate excitotoxicity. EAAT1 is prominently expressedin the frontal cortex, hippocampus and basal ganglia and is also reported to be found inheart, placenta, lung and striated muscle. EAAT1 shares some pharmacologicalsimilarities with EAAT3 and both are potent antagonists that appear to specifically blocktransport mediated by EAAT2.EAAT1 transports L-glutamate and also L- and D-aspartate, acting as a symport byco-transporting sodium. EAAT1 is essential for terminating the postsynaptic action ofglutamate, by rapidly removing released glutamate from the synaptic cleft. Alternate Names: anti-GLAST antibody, anti-EA6 antibody,anti-EAAT1 antibody, anti-Excitatory amino acid transporter 1 antibody, anti-FLJ25094 antibody, anti-GLAST antibody,anti-GLAST1 antibody, anti-Glial high affinity glutamate transporter antibody,anti-High affinity neuronal glutamate transporter antibody, anti-Slc1a3 antibody,anti-Sodium dependent glutamate/aspartate transporter antibody.

Specificity: This antibody reacts with Glutamate-aspartate transporter in CNS samples. Species Reactivity: Cross-reacts with Rat. The % of homology for the sequence used during thedevelopment of antibodies is 85% (between GLAST from rat and GLAST from human).

Synonyms: EA6, EAAT1, GLAST, GLAST1, FLJ25094, Gmt1, Eaat1, GLU-T, GluT-1, MGluT1, GLAST-1, AI504299, B430115D02Rik, AF001784, CG3747, dEAAT, dEaat1, dEAAT1, Dglt-1, Dglut-1, DmelCG3747, EAAT, MGC140731, zgc:56661, zgc:77768, wu:fb98e05, wu:fd50b05, SLC1A3

Application Details

Application Notes: Suggested working dilutions: flow cytometry / FACS analysis 1:10-1:200,immunohistochemistry 1:50-1:500,Western Blot 1:200-1:5000,Immunocytochemistry

Purification: Serum

Buffer: Preservative: No preservative.

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Restrictions: For Research Use only

Publications

Rothstein, Dykes-Hoberg, Pardo et al.: "Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate." in: Neuron, Vol. 16, Issue 3, pp. 675-86, 1996 (PubMed).

Schmitt, Asan, Pueschel et al.: "Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 1, pp. 1-10, 1997 (PubMed).

Kobayashi, Millhorn: "Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells." in: Journal of neurochemistry, Vol. 76, Issue 6, pp. 1935-48, 2001 (PubMed).