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Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody

Details for Product No. ABIN151381, Supplier: Log in to see
Antigen
  • AF001784
  • AI504299
  • B430115D02Rik
  • CG3747
  • dEAAT
  • dEaat1
  • dEAAT1
  • Dglt-1
  • Dglut-1
  • Dmel\\CG3747
  • EA6
  • EAAT
  • Eaat1
  • EAAT1
  • GLAST
  • GLAST-1
  • GLAST1
  • GLU-T
  • GluT-1
  • Gmt1
  • MGluT1
  • slc1a3
  • SLC1A3
  • wu:fb98e05
  • wu:fd50b05
  • zgc:56661
  • zgc:77768
Epitope
C-Term, AA 500-542
38
14
10
8
7
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
75
45
30
14
Host
Rabbit
64
20
9
1
Clonality
Polyclonal
Conjugate
Un-conjugated
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
71
36
17
14
12
8
5
3
3
2
1
1
Supplier
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Immunogen A synthetic peptide made to a C-terminal portion of the rat SLC1A3 protein (between residues 500-542) [UniProt P24942]
Cross-Reactivity (Details) Based upon 100% immunogen sequence similarity, this antibody is predicted to cross-react with Bovine also.
Purification Immunogen affinity purified
Alternative Name SLC1A3 / EAAT1 (SLC1A3 Antibody Abstract)
Background Gene Symbol: SLC1A3
Molecular Weight Theoretical MW: 60 kDa
UniProt P24942
Application Notes Western Blot 2 μg/mL, Flow Cytometry 1:200-1:500, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:50-1:500, Immunohistochemistry-Frozen 1:10-1:500, Flow (Intracellular) 1:500
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Western blot Protocol for SLC1A3 antibody
Western Blot Protocol:
1. Perform SDS-PAGE on protein samples to be analyzed, loading 10-40 µg of total protein per lane.
. Electro-blot the proteins to a suitable membrane (PVDF or Nitrocellulose) according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain the membrane with Ponceau S (or a similar product) to assess transfer success. Mark molecular weight standards where appropriate.
. Thoroughly rinse the membrane of stain with TBST.
. Incubate the membrane in blocking buffer (5 % non-fat milk in TBST or 5 % BSA in TBST) as appropriate, for 60 minutes.
. Dilute the SLC1A3 primary antibody as appropriate in blocking buffer and incubate for 60 minute at room temperature to overnight at 4 degrees C with gently shaking.
. Wash the membrane in TBST three times for 10 minutes each.
. Incubate the membrane in the appropriate secondary antibody prepared in blocking buffer (as per manufacturer's instructions) and incubate for 60 minutes at room temperature.
. Wash the membrane in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
. Incubate the membrane in the appropriate detection reagent in accordance with the manufacturer's instructions and image the blot.Note: Tween-20 can be added to the blocking, wash and antibody dilution buffers to a final concentration of 0.05-0.1 %.Immunohistochemistry-Paraffin protocol for SLC1A3 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 60 minutes at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 degrees C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
Restrictions For Research Use only
Format Liquid
Concentration 0.32 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Supplier Images
Flow Cytometry (FACS) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody
Immunohistochemistry (IHC) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (Image 2)
Western Blotting (WB) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (Image 3)
 image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Intracellular stai...
 image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Staining of HEK293...
 image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Flow (Intracellular): EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Analysis using the...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Immunohistochemistry-Paraffin: EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - IHC-P ana...
Western Blotting (WB) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Western Blot: EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - Western blot analysis of S...
Immunofluorescence (IF) image for anti-Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3) (AA 500-542), (C-Term) antibody (ABIN151381) Immunocytochemistry/Immunofluorescence: EAAT1/GLAST-1/SLC1A3 Antibody [ABIN151381] - ...
Product cited in: Arnold, Salvatore: "Exercise-Mediated Increase in Nigral Tyrosine Hydroxylase Is Accompanied by Increased Nigral GFR-α1 and EAAC1 Expression in Aging Rats." in: ACS chemical neuroscience, Vol. 7, Issue 2, pp. 227-39, 2016 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Pinner, Tucholski, Haroutunian, McCullumsmith, Meador-Woodruff: "Decreased protein S-palmitoylation in dorsolateral prefrontal cortex in schizophrenia." in: Schizophrenia research, Vol. 177, Issue 1-3, pp. 78-87, 2016 (PubMed). (Sample species: Human). Further details: Western Blotting

Reyes-Aguirre, Lamas: "Oct4 Methylation-Mediated Silencing As an Epigenetic Barrier Preventing Müller Glia Dedifferentiation in a Murine Model of Retinal Injury." in: Frontiers in neuroscience, Vol. 10, pp. 523, 2016 (PubMed). (Sample species: Human).

Chotibut, Davis, Arnold, Frenchek, Gurwara, Bondada, Geddes, Salvatore: "Ceftriaxone increases glutamate uptake and reduces striatal tyrosine hydroxylase loss in 6-OHDA Parkinson's model." in: Molecular neurobiology, Vol. 49, Issue 3, pp. 1282-92, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Vollbrecht, Simmler, Blakely, Deutch: "Dopamine denervation of the prefrontal cortex increases expression of the astrocytic glutamate transporter GLT-1." in: Journal of neurochemistry, Vol. 130, Issue 1, pp. 109-14, 2014 (PubMed). (Sample species: Rat (Rattus)). Further details: Western Blotting

Hu, Takano, Xiang, Gilkes, Luo, Semenza: "Hypoxia-inducible factors enhance glutamate signaling in cancer cells." in: Oncotarget, Vol. 5, Issue 19, pp. 8853-68, 2014 (PubMed). (Sample species: Human). Further details: Western Blotting

Salvatore, Davis, Arnold, Chotibut: "Transient striatal GLT-1 blockade increases EAAC1 expression, glutamate reuptake, and decreases tyrosine hydroxylase phosphorylation at ser(19)." in: Experimental neurology, Vol. 234, Issue 2, pp. 428-36, 2012 (PubMed).

Kobayashi, Millhorn: "Hypoxia regulates glutamate metabolism and membrane transport in rat PC12 cells." in: Journal of neurochemistry, Vol. 76, Issue 6, pp. 1935-48, 2001 (PubMed).

Background publications Schmitt, Asan, Pueschel, Kugler: "Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats: nonradioactive in situ hybridization and comparative immunocytochemistry." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 17, Issue 1, pp. 1-10, 1997 (PubMed).

Rothstein, Dykes-Hoberg, Pardo, Bristol, Jin, Kuncl, Kanai, Hediger, Wang, Schielke, Welty: "Knockout of glutamate transporters reveals a major role for astroglial transport in excitotoxicity and clearance of glutamate." in: Neuron, Vol. 16, Issue 3, pp. 675-86, 1996 (PubMed).

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