Drebrin 1 (DBN1) antibody

Antigen: Drebrin 1 (DBN1)

Synonyms: D0S117E, DKFZp434D064, DBN1, MGC138067, LOC733967

Clonality: Monoclonal (M2F6)

Host: Mouse

Reactivity: Chicken

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Catalog no.: ABIN151450

Additional Information

Alternative name: Drebrin

Gene ID: 1627, 56320, 81653

Immunogen: Chicken Drebrin E protein.

Cross-Reactivity: Chicken, Cat (Feline), Guinea Pig, Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG

Clone: M2F6

Description: Drebrins are actin-binding proteins, that are developmentally regulated in the process ofneuronal growth. Three isoforms, E1 and E2 (embryonic) and A (adult) are generated byalternative RNA splicing from a single debrin gene in the chicken brain. The expressionpattern of each isoform is regulated spatially and temporally in the developing brain. Ithas been suggested that debrin is one of the intracellular regulators of the neuronalmorphogenesis (4). Debrin has also been detected in other kinds of non-neuronalhuman and bovine tissue but its exact role is unknown. Alternate Names: anti-D0S117E antibody, anti-DBN 1 antibody, anti-DBN1 antibody, anti-Developmentallyregulated brain protein antibody, anti-DKFZp434D064 antibody, anti-Drebrin 1 antibody, anti-Drebrin E antibody, anti-Drebrin1 antibody, DrebrinE antibody.

Specificity: This antibody detects Drebrin A and E in samples from human, mouse, rat, guinea pig,and cat origins and Drebrin E only in chicken samples. Species Reactivity: Cross-reacts with Human, Mouse, Rat, Cat, Chicken and Guinea pig. Not yet tested inother species. Localization: Cytoplasmic.

Application Details

Application Notes: Suggested working dilutions: immunohistochemistry 10 ug/ml,Western Blot 10 ug/ml. Positive Controls: Mouse Brain Tissue Extract.

Concentration: 1 mg/ml

Purification: Purified

Buffer: PBS with 10mg/ml Sucrose [pH 7.2]. Preservative: No preservative

Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Research Area: Cancer, Cytoskeleton, Microfilaments, Alzheimer's Disease

Restrictions: For Research Use only

Images

Western Blot: Drebrin Antibody (M2F6) [ABIN151450] - Western blot analysis of mouse brain tissue extract probed with ABIN151450

Publications

Kojima, Kato, Shirao et al.: "Nucleotide sequences of two embryonic drebrins, developmentally regulated brain proteins, and developmental change in their mRNAs." in: Brain research, Vol. 464, Issue 3, pp. 207-15, 1989 (PubMed).

Shirao: "The roles of microfilament-associated proteins, drebrins, in brain morphogenesis: a review." in: Journal of biochemistry, Vol. 117, Issue 2, pp. 231-6, 1995 (PubMed).

Peitsch, Grund, Kuhn et al.: "Drebrin is a widespread actin-associating protein enriched at junctional plaques, defining a specific microfilament anchorage system in polar epithelial cells." in: European journal of cell biology, Vol. 78, Issue 11, pp. 767-78, 2000 (PubMed).

Maezawa, Nivison, Montine et al.: "Neurotoxicity from innate immune response is greatest with targeted replacement of E4 allele of apolipoprotein E gene and is mediated by microglial p38MAPK." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 20, Issue 6, pp. 797-9, 2006 (PubMed).