Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody

Details for Product No. ABIN151451
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Antigen
Synonyms AI196048, CD107a, Lamp-1, LAMPA, LGP120, LEP100
Reactivity
Hamster
(146), (69), (58), (7), (7), (3), (3), (2)
Host
Mouse
(96), (83), (31), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(26), (17), (14), (10), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
(116), (94), (48), (44), (41), (41), (31), (26), (19), (10), (1), (1)
Pubmed 5 references available
Quantity 0.05 mg
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Catalog No. ABIN151451
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Immunogen Rat liver lysosomal membrane preparations (NP_036989.1).
Clone LY1C6
Isotype IgG1
Specificity Detects an approx.120kDa protein corresponding to the molecular mass of LAMP1 on SDS-PAGE immunoblots.
Purification Protein G purified
Alternative Name CD107a / LAMP1
Background Lysosome-associated membrane proteins (LAMP1), also known as lgp120 or lgpA, is atype 1 integral membrane protein that is transported from trans-Golgi network toendosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasmamembrane is dependent on a carboxyl-terminal tyrosine based motif(YXXI). Perturbation in the spacing between the tyrosine based motif relative to themembrane abolishes lysosome localization of LAMP1. This mutant protein then cyclesbetween the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2have beenshown to promote adhesion of human peripheral blood mononuclear cells (PBMC) tovascular endothelium, therefore they are possiblyinvolved in the adhesion of PBMC to the site of inflammation. Alternate Names: anti-CD107a antibody, anti-CD107a antigen antibody, anti-LAMP1 antibody,anti-LAMPA antibody, anti-LGP120 antibody, anti-lgpA antibody, anti-Lysosomalmembrane glycoprotein 120KD antibody, anti-Lysosomal Associated Membrane Protein1 antibody.
Gene Symbol: LAMP1
Gene ID 3916
NCBI Accession NP_036989
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Recommended dilutions: Electron Microscopy 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500, Western Blot 1 µg/mL
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS [pH 7.2] with 50 % glycerol, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
General Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Kannan, Stewart, Bounds et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996 (PubMed).

Jones, Jiang, Yamamoto et al.: "Tuberin is a component of lipid rafts and mediates caveolin-1 localization: role of TSC2 in post-Golgi transport." in: Experimental cell research, Vol. 295, Issue 2, pp. 512-24, 2004 (PubMed).

Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

Liu, Zhang, Shi et al.: "Rab11a and HSP90 regulate recycling of extracellular alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 5, pp. 1480-5, 2009 (PubMed).

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