Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody

Antigen: Lysosomal-Associated Membrane Protein 1 (LAMP1)

Synonyms: LAMPA, CD107a, LGP120

Clonality: Monoclonal (LY1C6)

Host: Mouse

Reactivity: Hamster

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Catalog no.: ABIN151451

Additional Information

Alternative name: CD107a / LAMP1

Immunogen: Rat liver lysosomal membrane preparations.

Cross-Reactivity: Hamster, Rat (Rattus)

Isotype: IgG1

Clone: LY1C6

Description: Lysosome-associated membrane proteins (LAMP1), also known as lgp120 or lgpA, is atype 1 integral membrane protein that is transported from trans-Golgi network toendosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasmamembrane is dependent on a carboxyl-terminal tyrosine based motif(YXXI). Perturbation in the spacing between the tyrosine based motif relative to themembrane abolishes lysosome localization of LAMP1. This mutant protein then cyclesbetween the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2have beenshown to promote adhesion of human peripheral blood mononuclear cells (PBMC) tovascular endothelium, therefore they are possiblyinvolved in the adhesion of PBMC to the site of inflammation. Alternate Names: anti-CD107a antibody, anti-CD107a antigen antibody, anti-LAMP1 antibody,anti-LAMPA antibody, anti-LGP120 antibody, anti-lgpA antibody, anti-Lysosomalmembrane glycoprotein 120KD antibody, anti-Lysosomal Associated Membrane Protein1 antibody.

Specificity: Detects an approx. 120kDa proteincorresponding to the molecular mass of LAMP1 on SDS-PAGE immunoblots. Species Reactivity: Cross reacts with Rat and Hamster. Localization: Type I membrane protein. Lysosomal. This protein shuttles between lysosomes,endosomes, and the plasma membrane.

Application Details

Application Notes: Suggested working dilutions: immunoprecipitation 5ug/ml,Western Blot 1 ug/ml,Immunocytochemistry 5-10 ug/ml. Positive Controls: Highly purified rat lysosomal membrane preparations.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS [pH7.2] with 50% glycerol

Storage: Aliquot and store at -20C or -80C. Avoid freeze thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Kannan, Stewart, Bounds et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996 (PubMed).

Jones, Jiang, Yamamoto et al.: "Tuberin is a component of lipid rafts and mediates caveolin-1 localization: role of TSC2 in post-Golgi transport." in: Experimental cell research, Vol. 295, Issue 2, pp. 512-24, 2004 (PubMed).

Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

Liu, Zhang, Shi et al.: "Rab11a and HSP90 regulate recycling of extracellular alpha-synuclein." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 5, pp. 1480-5, 2009 (PubMed).