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CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (AA 9-26) antibody

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Synonyms CTCFL, BORIS, CT27, CTCF-T, HMGB1L1, dJ579F20.2, Boris, OTTMUSG00000016680
AA 9-26
(7), (5), (1), (1), (1), (1)
(45), (26), (24), (2)
(49), (2), (1), (1)
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), ELISA
(39), (11), (10), (10), (8), (3), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Quantity 0.1 mg
Shipping to United States ( )
Immunogen Synthetic peptide (Human) - amino acids 9-26 of human BORIS
Specificity BORIS
Cross-Reactivity (Details) Expected to cross react with chimpanzee. May react with mouse, dog,rat,sources based on sequence identity. Not yet tested in other species.
Purification affinity purified
Alternative Name CTCFL / BORIS (CTCFL Antibody Abstract)
Background BORIS (Brother of the Regulator of Imprinted Sites) also known as CCTC-bindingfactor-like protein, is normally only expressed in the testis and expressed in a mutuallyexclusive manner with CTCF during male germ cell development. However, previousstudies have shown that BORIS is abnormally activated in a wide range of humancancers. Expression of BORIS in normally BORIS-negative cells promotes cell growththat may lead to transformation. BORIS maps to the cancer-associated amplificationregion thought to contain an oncogene or dominant-immortalizing gene. BORIS is acandidate protein for the epigenetic reprogramming factor acting in the male germ line.BORIS is found in both the nucleus and cytoplasm. Alternate Names: anti-Brother Of The Regulator Of Imprinted Sites antibody.
Gene Symbol: CTCFL
Gene ID 140690
Application Notes This affinity purified antibody has been tested for use in ELISA and by western blot. Expect a predominant band approximately 75 kDa in size corresponding - BORIS by western blotting in the appropriate cell lysate or extract.
Recommended dilutions: ELISA 1:2000-1:10000, Western Blot 1:200-1:2000
Restrictions For Research Use only
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium chloride pH 7.2, Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Aliquot and store at -20 °C or -80 °C.
Supplier Images
Western Blotting (WB) image for anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (AA 9-26) antibody (ABIN151496) anti-CCCTC-Binding Factor (Zinc Finger Protein)-Like (CTCFL) (AA 9-26) antibody
Product cited in: Monk, Hitchins, Hawes: "Differential expression of the embryo/cancer gene ECSA(DPPA2), the cancer/testis gene BORIS and the pluripotency structural gene OCT4, in human preimplantation development." in: Molecular human reproduction, Vol. 14, Issue 6, pp. 347-55, 2008 (PubMed).

Klenova, Morse, Ohlsson et al.: "The novel BORIS + CTCF gene family is uniquely involved in the epigenetics of normal biology and cancer." in: Seminars in cancer biology, Vol. 12, Issue 5, pp. 399-414, 2002 (PubMed).

Catalog No. ABIN151496
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